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Cellular and Molecular Life Sciences CMLS

, Volume 53, Issue 1, pp 73–77 | Cite as

Fluorescence replication banding of frog chromosomes

  • I. Miura
  • H. Ohtani
  • M. Nakamura
  • K. Saitoh

Abstract.

To identify individual chromosomes of a frog karyotype by their fluorescence banding patterns, chromosomes were stained with actinomycin D and 4,6-diamidino-2-phenylindole (DAPI) after incorporation of BrdU during the late S-phase. The chromosomes of three Rana species which were selected for this study (R. ridibunda, R. lessonae and R. japonica) showed well-defined late replication bands. The fluorescence patterns obtained were the reverse of those produced by a 4Na-EDTA Giemsa-staining technique. Fluorescence patterns of the two water frog species (R. ridibunda and R. lessonae) were similar to each other, except for the different fluorescence of the centromeric heterochromatin, which gave extremely bright signals in R. ridibunda but no signal in R. lessonae. Experiments also showed differences between the fluorescence patterns of R. lessonae chromosome 13 in the Italian and Luxembourgian populations. These results sho w that the fluorescence replication banding using actinomycin D and DAPI is very effective in identifying individual frog chromosomes and detecting their structural changes.

Key words. Late replication banding; DAPI; actinomycin D; frogs. 

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Copyright information

© Birkhäuser Verlag Basel, 1997

Authors and Affiliations

  • I. Miura
    • 1
  • H. Ohtani
    • 1
  • M. Nakamura
    • 1
  • K. Saitoh
    • 2
  1. 1.Laboratory for Amphibian Biology, Faculty of Science, Hiroshima University, Higashihiroshima 739 (Japan), Fax +81 824 24 0739, e-mail: imiura@ue.ipc.hiroshima-u.ac.jpJP
  2. 2.Department of Bioscience and Biotechnology, Faculty of Engineering, Aomori University, Aomori 030 (Japan)JP

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