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Inflammation Research

, Volume 50, Issue 12, pp 616–624 | Cite as

The effect of allergen-induced airway inflammation on airway remodeling in a murine model of allergic asthma

  • H. Tanaka
  • T. Masuda
  • S. Tokuoka
  • M. Komai
  • K. Nagao
  • Y. Takahashi
  • H. Nagai

Abstract.

Objective and design: We examined the effect of airway inflammation on airway remodeling and bronchial responsiveness in a mouse model of allergic asthma.¶Materials and methods: BALB/c mice were sensitized to ovalbumin (OA), and exposed to aerosolized OA (0.01, 0.1 and 1%). Twenty-four hours after the final antigen challenge, bronchial responsiveness was measured, and bronchoalveolar lavage (BAL) and histological examinations were carried out.¶Results: Repeated antigen exposure induced airway inflammation, IgE/IgG1 responses, epithelial changes, collagen deposition in the lungs, subepithelial fibrosis associated with increases in the amount of transforming growth factor (TGF)-β1 in BAL fluid (BALF), and bronchial hyperresponsiveness to acetylcholine. The number of eosinophils in BALF was significantly correlated with TGF-β1 production in BALF and the amount of hydroxyproline. Furthermore, significant correlations were found between these fibrogenic parameters and the bronchial responsiveness.¶Conclusion: These findings demonstrated that in this murine model airway eosinophilic inflammation is responsible for the development of airway remodeling as well as bronchial hyperresponsiveness in allergic bronchial asthma.

Key words: Airway inflammation - Bronchial hyperresponsiveness - Goblet cells - Subepithelial fibrosis - TGF-β1 

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Copyright information

© Birkhäuser Verlag, 2001

Authors and Affiliations

  • H. Tanaka
    • 1
  • T. Masuda
    • 1
  • S. Tokuoka
    • 1
  • M. Komai
    • 1
  • K. Nagao
    • 1
  • Y. Takahashi
    • 1
  • H. Nagai
    • 1
  1. 1.Department of Pharmacology, Gifu Pharmaceutical University, 5-6-1 Mitahora-higashi, Gifu 502-8585, Japan, Fax: ++ 81 58 237 8584, e-mail: nagai@gifu-pu.ac.jp JP

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