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Bioaktive Glas-Scaffolds beschichtet mit Zein, einem pflanzlichen Protein: Entwicklung und Eigenschaften

  • Forschung & Technik
  • Published:
Keramische Zeitschrift

Kurzfassung

Die vorliegende Studie beschreibt die Beschichtung poröser Gerüststrukturen, sogenannter Scaffolds (auf Basis von 45S5 bioaktivem Glas), mit Zein, einem Speicherprotein gewonnen aus Mais. Da bioaktive Scaffolds durch ihren Herstellungsprozess Mikrorisse an der Oberfläche aufzeigen, sind ihre mechanischen Festigkeiten extrem schwach. Durch Auftragen einer Polymerschicht können diese Risse gefüllt und überbrückt werden, was zur Erhöhung der Bruchfestigkeit und -arbeit führt. Dazu werden gesinterte Glas-Scaffolds in eine Lösung aus Zein getaucht. Nach dem Abdampfen des Lösemittels bleibt ein dünner Polymerfilm von ~10 μm auf der Oberfläche zurück. Die Mikrostruktur und Morphologie wurde anschließend mit Rasterelektronenmikroskopie (REM) untersucht. Des Weiteren konnte durch Auslagerung in simulierte Körperflüssigkeit (SBF) der Einfluss der Beschichtung auf das bioaktive Verhalten der Scaffolds getestet werden. Der Nachweis für die Bildung von Hydroxylapatit wurde durch REM und Fourier-Transform-Infrarotspektrometrie (FTIR) erbracht. Auch konnte die Druckfestigkeit durch das Aufbringen einer Zeinschicht um den Faktor 5 erhöht werden.

Abstract

Bioactive Glass-based Scaffolds Coated with Zein, a Plant-derived Protein: Development and Characteristics

The present study describes the coating of porous scaffolds (based on 45S5 bioactive glass and produced by the foam replica method) with zein, a storage protein derived from corn. As bioactive scaffolds show microcracks on the surface, the mechanical performance is very weak. By applying a polymer layer, these cracks can be filled and bridged, which leads to enhanced fracture strength and toughness. Therefore, bioactive glass-based scaffolds are immersed in a zein solution. After evaporation of the solvent, a thin polymer film of ~10 μm remains on the surface. The microstructure and morphology were investigated by scanning electron microscopy (SEM). Furthermore, the influence of the coating on the bioactive behavior of the scaffolds was tested by immersion in simulated body fluid (SBF). The formation of hydroxyapatite was analyzed by SEM and Fourier Transform Infrared Spectrometry (FTIR). By applying a zein coating, the compressive strength was increased by factor 5.

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Authors and Affiliations

  1. Lehrstuhl Biomaterialien, Department Werkstoffwissenschaften, Universität Erlangen-Nürnberg, Cauerstraße 6, D-91058, Erlangen, Deutschland

    J. Hum, J. Potschka & A.R. Boccaccini

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Hum, J., Potschka, J. & Boccaccini, A. Bioaktive Glas-Scaffolds beschichtet mit Zein, einem pflanzlichen Protein: Entwicklung und Eigenschaften. Keram. Z. 69, 34–41 (2017). https://doi.org/10.1007/BF03400290

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