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Fungal cell wall degrading enzyme-producing bacterium enhances the biocontrol efficacy of antibiotic-producing bacterium against cabbage yellows

Ein Zellwand zersetzendes Bakterium steigert die antagonistische Wirkung eines Antibiotika bildenden Bakteriums gegenüber der Fusarium-Kohlwelke

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Abstract

The antagonistic rhizobacterium, Pseudomonas fluorescens strain LRB3W1, controls cabbage Fusarium yellows caused by Fusarium oxysporum f. sp. conglutinans and inhibits its growth in vitro. Conversely, Serratia marcescens strain B2, known as a biocontrol bacterium, did not inhibit growth of F. oxysporum f. sp. conglutinans and the suppressive effect of strain B2 against cabbage yellows was less than that of strain LRB3W1. However, combined inoculation of strain LRB3W1 with strain B2 was more effective than treatment with strain LRB3W1 alone. Colonization of each bacterium in the cabbage rhizosphere was not influenced by the presence of the other bacterium. When bud-cells of the pathogen were treated with 2,4-diacetylphloroglucinol from strain LRB3W1 and fungal cell wall degrading enzyme from strain B2 simultaneously, an additive inhibitory effect was observed. According to the results the fungal cell wall degrading enzyme-producing bacterium enhanced the biocontrol effect of the antibiotic-producing bacterium against cabbage Fusarium yellows.

Zusammenfassung

Der Stamm LRB3W1 des antagonistischen Rhizobacteriums Pseudomonas fluorescens kann zur Bekämpfung der durch Fusarium oxysporum f. sp. conglutinans verursachten Kohlwelke verwendet werden; er beeinträchtigt das Wachstum des Erregers in vitro. Im Gegensatz dazu vermindert das zur biologischen Bekämpfung bodenbürtiger Erreger eingesetzte Bakteri-um Serratia marcescens, Stamm B2, das Wachstum von Fusa-rium oxysporum f. sp. conglutinans nicht und seine suppressive Wirkung gegenüber der Fusarium-Kohlwelke ist geringer als der des P. fluorescens-Stammes LRB3W1. Eine gemeinsame Inokulation beider Stämme wiederum übertraf die Wirkung von P. fluorescens LRB3W1. Beide Stämme beeinträchtigten nicht die Kolonisierung der Rhizosphäre von Kohl durch den jeweils anderen. Eine kombinierte Behandlung von Keimschlauchzellen des Erregers der Fusarium-Kohlwelke mit 2,4-Diacetylphloroglucinol aus dem Stamm LWB3W1 und einem Zellwand abbauendem Enzym des Stammes B2 wirkte additiv antagonistisch. Die Ergebnisse zeigen, dass der Zellwände des Erregers der Fusarium-Kohlwelke abbauende Bakterienstamm die antagonistische Wirkung des Antibiotika bildenden Stammes auf dieses Pathogen erhöht.

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Author notes

  1. K. Tsuchiya

    Present address: National Agricultural Research Center for Western Region, Sen-yu, Zentsuji, Kagawa, 765-8508, Japan

  2. H. Sawada

    Present address: National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, Ibaraki, 305-8602, Japan

Authors and Affiliations

  1. National Institute for Agro-Environmental Sciences, Kannondai, Tsukuba, Ibaraki, 305-8604, Japan

    N. Someya, K. Tsuchiya, T. Yoshida, M. T. Noguchi & H. Sawada

  2. School of Agriculture, Ibaraki University, Ami-machi, Ibaraki, Japan

    K. Akutsu

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  1. N. Someya
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  2. K. Tsuchiya
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  3. T. Yoshida
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  5. K. Akutsu
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Correspondence to N. Someya.

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Someya, N., Tsuchiya, K., Yoshida, T. et al. Fungal cell wall degrading enzyme-producing bacterium enhances the biocontrol efficacy of antibiotic-producing bacterium against cabbage yellows. J Plant Dis Prot 114, 108–112 (2007). https://doi.org/10.1007/BF03356716

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  • DOI: https://doi.org/10.1007/BF03356716

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