Journal of Plant Diseases and Protection

, Volume 114, Issue 3, pp 101–107 | Cite as

Screening banana plants for banana bunchy top virus with primers specific to Indian isolates

  • J. Anandhi
  • C. Vijila
  • G. S. Viswanath
  • T. S. Lokeswari


The banana bunchy top disease is a serious threat to banana production. Caused by a DNA virus, its detection using specific primers is the only sensitive method to certify mother plants for tissue culture. Newer strains and variants of this virus were reported based on severity of symptoms and difference in the sizes of amplicons in a PCR assay. A search for newer strains or variants of this virus was therefore attempted in this study. We designed primers unique to the stem-loop region of DNA 2 from clones of this virus occurring in Indian Musa spp. These and other primers obtained from the literature were used to screen plants in the field and tissue cultured propagules. The results presented here report the occurrence of at least two distinct strains showing variation in sequences of DNA 2. The likely origin of this variant sequence is discussed here.

Key words

BBTV DNA 2 major common region Musa spp. PCR assay stem-loop region 


BBTV DNA 2 Major Common Region Musa spp. PCR-Analyse Stem-Loop-Region 

Screening von Bananenpflanzen auf das Banana bunchy top virus mit für indische Isolate spezifischen Primern


Das Banana bunchy top virus stellt eine ernsthafte Bedrohung für den Bananenanbau dar. Ein sicherer Nachweis dieses DNA-Virus im Rahmen der Zertifizierung von Mutterpflanzen für die Gewebekultur gelingt nur mit Hilfe spezifischer Primer. Neuere Virusstämme und -varianten wurden anhand der Ausprägung von Schadsymptomen und der Größe von PCR-Amplicons beschrieben. Unsere Untersuchung hatte die Suche nach neuen Virusstämmen und -varianten zum Ziel. Zu diesem Zweck konstruierten wir Primer aus Virusklonen indischer Musa-Arten mit einer Spezifität für die Stem-Loop-Region der DNA 2. Diese und andere in der Literatur beschriebene Primer wurden zum Screening von Pflanzen im Feld und aus Gewebekulturen verwendet. Mindestens zwei verschiedene Virusstämme mit abweichenden DNA-2-Sequenzen konnten so nachgewiesen werden. Die Herkunft dieser abweichenden Sequenzen wird diskutiert.


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Copyright information

© Deutsche Phythomedizinische Gesellschaft 2007

Authors and Affiliations

  • J. Anandhi
    • 1
  • C. Vijila
    • 1
  • G. S. Viswanath
    • 1
  • T. S. Lokeswari
    • 1
  1. 1.Sri Ramachandra College of Biomedical SciencesTechnology and Research, Sri Ramachandra UniversityChennaiIndia

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