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Screening banana plants for banana bunchy top virus with primers specific to Indian isolates

Screening von Bananenpflanzen auf das Banana bunchy top virus mit für indische Isolate spezifischen Primern

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Abstract

The banana bunchy top disease is a serious threat to banana production. Caused by a DNA virus, its detection using specific primers is the only sensitive method to certify mother plants for tissue culture. Newer strains and variants of this virus were reported based on severity of symptoms and difference in the sizes of amplicons in a PCR assay. A search for newer strains or variants of this virus was therefore attempted in this study. We designed primers unique to the stem-loop region of DNA 2 from clones of this virus occurring in Indian Musa spp. These and other primers obtained from the literature were used to screen plants in the field and tissue cultured propagules. The results presented here report the occurrence of at least two distinct strains showing variation in sequences of DNA 2. The likely origin of this variant sequence is discussed here.

Zusammenfassung

Das Banana bunchy top virus stellt eine ernsthafte Bedrohung für den Bananenanbau dar. Ein sicherer Nachweis dieses DNA-Virus im Rahmen der Zertifizierung von Mutterpflanzen für die Gewebekultur gelingt nur mit Hilfe spezifischer Primer. Neuere Virusstämme und -varianten wurden anhand der Ausprägung von Schadsymptomen und der Größe von PCR-Amplicons beschrieben. Unsere Untersuchung hatte die Suche nach neuen Virusstämmen und -varianten zum Ziel. Zu diesem Zweck konstruierten wir Primer aus Virusklonen indischer Musa-Arten mit einer Spezifität für die Stem-Loop-Region der DNA 2. Diese und andere in der Literatur beschriebene Primer wurden zum Screening von Pflanzen im Feld und aus Gewebekulturen verwendet. Mindestens zwei verschiedene Virusstämme mit abweichenden DNA-2-Sequenzen konnten so nachgewiesen werden. Die Herkunft dieser abweichenden Sequenzen wird diskutiert.

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Literature

  • Anandhi, J., 2006: Detection and molecular characterization of Indian isolates of banana bunchy top virus. PhD thesis, University of Madras.

    Google Scholar 

  • Austin, H.L., 2004: Birth and death evolution of protein coding regions and concerted evolution of non-coding regions in the multicomponent genomes of nanoviruses. Mol. Phylogenet. Evol. 30, 287–294.

    Article  Google Scholar 

  • Beetham, P.R., R.M. Harding, J.L. Dale, 1999: Banana bunchy top virus DNA 2–6 are monocistronic. Arch. Virol. 144, 89–105.

    Article  CAS  PubMed  Google Scholar 

  • Hafner, G.J., R.M. Harding, J.L. Dale, 1997: A DNA primer associated with banana bunchy top virus. J. Gen. Virol. 78, 479–486.

    Article  CAS  PubMed  Google Scholar 

  • Hu, J., H. Fu, C. Lin, H. Su, H.H. Yeh, 2007: Reassortment and concerted evolution in banana bunchy top virus genomes. J. Virol. 81, 1746–1761.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Karan, M., R.M. Harding, J.L. Dale, 1997: Association of Banana bunchy top virus DNA components 2 to 6 with bunchy top disease. Plant Pathology Online, http://www.bspp.org.uk/mppo/1997/0624karan.

    Google Scholar 

  • Magee, C.J.P., 1953: Some aspects of the bunchy top disease of banana and other Musa spp. J. Proc. Royal Soc. N. S. W. 87, 1–18.

    Google Scholar 

  • Mansoor, S., J. Qazi, I. Amin, A. Khatri, I.A. Khan, S. Raza, Y. Zafar, R.W. Briddon, 2005: A PCR-based method, with internal control, for the detection of banana bunchy top virus in banana. J. Gen. Virol. 86, 1851–1860.

    Article  Google Scholar 

  • Sambrook, J., D.W. Russell, 2001: Molecular Cloning: A Laboratory Manual. Third edition. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA.

    Google Scholar 

  • Su, H.J., L.Y. Tsao, M.L. Wu, T.H. Hung, 2003: Biological and molecular categorization of strains of banana bunchy top virus. J. Phytopathol. 151, 290–296.

    Article  CAS  Google Scholar 

  • Thomas, J.E., 1991: Virus indexing procedures for banana in Australia. In: R.V. Valmayor, B.E. Umali, C.P. Bejosano (eds.): Banana Diseases in Asia and the Pacific, pp 144–155. International Network for the Improvement of Banana and Plantain, Montpellier, France.

    Google Scholar 

  • Valmayor, R.V., B.E. Umali, C.P. Bejosana, 1991: Banana Diseases in Asia and the Pacific. International Network for the Improvement of Banana and Plantain, Montpellier, France.

    Google Scholar 

  • Wanitchakorn, R., R.M. Harding, J.L. Dale, 2000: Sequence variability in the coat protein gene of two groups of banana bunchy top isolates. Arch. Virol. 145, 593–602.

    Article  CAS  PubMed  Google Scholar 

  • Wu, R.Y., H.J. Su, 1990: Purification and characterization of banana bunchy top virus. J. Phytopathol. 128, 153–160.

    Article  Google Scholar 

  • Xie, W.S., J.S. Hu, 1995: Molecular cloning, sequence analysis, and detection of banana bunchy top virus in Hawaii. Phytopathology 85, 339–347.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Sri Ramachandra College of Biomedical Sciences, Technology and Research, Sri Ramachandra University, No. 1, Ramachandra Nagar, Porur, Chennai, 600 116, Tamil Nadu, India

    J. Anandhi, C. Vijila, G. S. Viswanath & T. S. Lokeswari

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  1. J. Anandhi
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  2. C. Vijila
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  3. G. S. Viswanath
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  4. T. S. Lokeswari
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Correspondence to T. S. Lokeswari.

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Anandhi, J., Vijila, C., Viswanath, G.S. et al. Screening banana plants for banana bunchy top virus with primers specific to Indian isolates. J Plant Dis Prot 114, 101–107 (2007). https://doi.org/10.1007/BF03356715

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  • DOI: https://doi.org/10.1007/BF03356715

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