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Parasitology Research

, Volume 84, Issue 6, pp 509–512 | Cite as

Synergy between Entamoeba histolytica and Escherichia coli in the induction of cytokine gene expression in human colon epithelial cells

  • Jung Mogg Kim
  • Hyun Chae Jung
  • Kyung-il Im
  • In Sung Song
  • Chung Yong Kim
Original Paper

Abstract

Entamoeba histolytica induces the expression and secretion of interleukin 8 (IL-8) by cultured colon epithelial cells. We assessed the array of cytokine genes expressed by human colon epithelial cells in response to co-culture with E. histolytica trophozoites and tested the hypothesis that enteric bacteria may alter the E. histolytica-induced expression of such genes. HT-29 colon epithelial cells were co-cultured with E. histolytica trophozoites in the presence or absence of Escherichia coli. Cytokine gene expression was assessed by quantitative reverse-transcription polymerase chain reaction (RT-PCR). IL-8 mRNA in colon epithelial cells was up-regulated following exposure to E. histolytica and this was paralleled by increased IL-8 secretion. Granulocyte-macrophage colony-stimulating factor (GM-CSF) and IL-1α/β mRNAs were also up-regulated in these cells. When HT-29 cells were co-cultured with E. coli DH5α and E. histolytica there was a synergistic increase in the expression of IL-8, IL-1α, and GM-CSF. These results suggest that enteric bacteria may significantly affect early proinflammatory signals produced in host tissues in response to E. histolytica infection.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • Jung Mogg Kim
    • 1
  • Hyun Chae Jung
    • 2
  • Kyung-il Im
    • 3
  • In Sung Song
    • 2
  • Chung Yong Kim
    • 2
  1. 1.Department of MicrobiologyHanyang University College of MedicineSeoulSouth Korea
  2. 2.Department of Internal Medicine and Liver Research InstituteSeoul National University College of MedicineSeoulSouth Korea
  3. 3.Department of Parasitology and Institute of Tropical MedicineYonsei University College of MedicineSeoulSouth Korea

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