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Quantification of Wheat spindle streak mosaic virus and Soil borne cereal mosaic virus in resistance testing of field samples of triticale using real-time RT-PCR

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Abstract

Triticale, a cross between rye and wheat, is a crop important for animal feed and the production of biogas and ethanol. Soil-borne viruses found in wheat and rye, such as Furoviruses and Bymoviruses, also infect triticale. In order to evaluate resistance/tolerance it is necessary to accurately quantify virus content in the plants. We have developed RT-qPCR assays for the quantitative detection of the Bymovirus Wheat spindle streak mosaic Virus (WSSMV) and the Furovirus Soil-borne cereal mosaic virus (SBCMV) in field samples. Both a SYBR Green and a hydrolysis probe approach were tested. The RT-qPCR approach allows the quantitative evaluation and differentiation of triticale resistance to WSSMV and SBCMV. The reproducible large-scale analysis of field samples is feasible.

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Authors and Affiliations

  1. JKI, Institute for Epidemiology and Pathogen Diagnostics, Erwin-Baur-Str. 27, 06484, Quedlinburg, Germany

    Angelika Ziegler, Karin Klingebeil, Viola Papke & Ute Kastirr

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  1. Angelika Ziegler
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  2. Karin Klingebeil
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  3. Viola Papke
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  4. Ute Kastirr
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Correspondence to Angelika Ziegler.

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Ziegler, A., Klingebeil, K., Papke, V. et al. Quantification of Wheat spindle streak mosaic virus and Soil borne cereal mosaic virus in resistance testing of field samples of triticale using real-time RT-PCR. J Plant Dis Prot 121, 149–155 (2014). https://doi.org/10.1007/BF03356503

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