Skip to main content
SpringerLink
Log in

Impact of arbuscular mycorrhiza on the St. John’s wort (Hypericum perforatum) wilt disease induced by Colletotrichum cf. gloeosporioides

  • Published:
Journal of Plant Diseases and Protection Aims and scope Submit manuscript

Abstract

The importance of herbal plants is evident in the prevalent use as flavoring ingredients in food. However, meeting the growing demand for organic grown spices and ‘medicinal plants’ of regional origin is often hampered by technical difficulties during cultivation. Arbuscular mycorrhizal fungi (AMF) can support their host, by helping them to adapt to prevailing local conditions and thus increase the health of the plants.

The aim of the present work was to evaluate the effects of arbuscular mycorrhiza (AMF) on the plant’s health, using St. John’s wort (Hypericum perforatum) - Colletotrichum cf. gloeosporioides (Cfg) as the model plant pathosystem.

Following inoculation with AMF, the attack of St. John’s wort with Cfg led to a clear reduction in wilting of the two St. John’s wort cultivars. Furthermore, the yield of mycor-rhizal plants increased compared to non-mycorrhizal plants, irrespective of whether they were pathogen-infected or not. Compared to non-mycorrhizal plants, in mycorrhizal plants levels of ascorbic acid were elevated and activity of antioxidant enzymes increased after inoculation with Cfg. Furthermore, in mycorrhizal plants the progress in lipid peroxidation following pathogen attack was reduced, suggesting that the reduction of lipid peroxidation and the induction of antioxidants may play a crucial role in the plant’s defense against Cfg.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Abdel Latef AA, 2011. Influence of arbuscular mycorrhizal fungi and copper on growth, accumulation of osmolyte, mineral nutrition and antioxidant enzyme activity of pepper (Capsicum annuum L.). Mycorrhiza (ahead of print). Aebi H, 1984. Catalase in vitro. Methods Enzymol 105, 121–126.

    Google Scholar 

  • Asada K, 1992. Ascorbate peroxidase — a hydrogen peroxidase-scavenging enzyme in plants. Physiol Plantarum 85, 235–241.

    Article  CAS  Google Scholar 

  • Azcón-Aguilar C, Jaizme-Vega MC & Calvet C, 2002. The contribution of arbuscular mycorrhizal fungi to the control of soil-borne plant pathogens. In: Gianinazzi S, Schüepp H, Barea JM, Haselwandter K (eds.) Mycorrhizal technology in agriculture. Birkhäuser-Verlag, Switzerland, pp 187–197.

    Chapter  Google Scholar 

  • Baltruschat H, Fodor J, Harrach BD, Niemczyk E, Barna B, Gullner G, Janeczko A, Kogel KH, Schäfer P, Schwarczinger I, Zuccaro A & Skoczowski A, 2008. Salt tolerance of barley induced by the root endophyte Piriformospora indica is associated with a strong increase in antioxidants. New Phytol 180, 501–510.

    Article  CAS  PubMed  Google Scholar 

  • Blilou I, Bueno P, Ocampo JA & Garcia-Garrido JM, 2000. Induction of catalase and ascorbate peroxidase activities in tabacco roots inoculated with the arbuscular mycorrhizal Glomus mossae. Mycol Res 104, 722–725.

    Article  CAS  Google Scholar 

  • Bødker L, Kjøller R, Kristensen K & Rosendahl S, 2002. Interactions between indigenous arbuscular mycorrhizal fungi and Aphanomyces euteiches in field-grown pea. Mycorrhiza 12, 7–12.

    Article  PubMed  Google Scholar 

  • Buchanan BB, Gruissem W & Jones RL, 2000. Biochemistry & Molecular Biology of Plants. American Society of Plant Physiologists, Rockville, USA.

    Google Scholar 

  • Campagnac E, Lounès-Hadj Sahraoui A, Debiane D, Fontaine J, Laruelle F, Garçon G, Verdin A, Durand R, Shirali P & Grandmougin-Ferjani A, 2010. Arbuscular mycorrhiza partially protect chicory roots against oxidative stress induced by two fungicides, fenpropimorph and fenhexamid. Mycorrhiza 20, 167–178.

    Article  CAS  PubMed  Google Scholar 

  • Chaudhary V, Kapoor R & Bhatnagar AK, 2008. Effectiveness of two arbuscular mycorrhizal fungi on concentrations of essential oil and artemisinin in three accessions of Artemisia annua L. Appl Soil Ecol 40, 174–181.

    Article  Google Scholar 

  • Chen KM, Gong HJ, Chen GC, Wang SM & Zhang CL, 2004. Gradual drought under field conditions influences the glutathione metabolism, redox balance and energy supply in spring wheat. J Plant Growth Regul 23, 20–28.

    Article  CAS  Google Scholar 

  • Fakhro A, Andrade-Linares DR, von Bargen S, Bandte M, Büttner C, Grosch R, Schwarz D & Franken P, 2010. Impact of Piriformospora indica on tomato growth and on interaction with fungal and viral pathogens. Mycorrhiza 20, 191–200.

    Article  PubMed  Google Scholar 

  • Gärber U, 2003. Johanniskrautwelke, Colletotrichum cf. gloeosporioides, Ergebnisse mehrjähriger Forschungsarbeiten im Überblick. Drogenreport 16, 23–28.

    Google Scholar 

  • Garmendia I, Goicoechea N & Aguirreolea J, 2004. Effectiveness of three Glomus species in protecting pepper (Capsicum annuum L.) against Verticillium wilt. Biol Control 31, 296–305.

    Article  Google Scholar 

  • Gianinazzi S, Gollotte A, Binet MN, van Tuinen D, Redecker D & Wipf D, 2010. Agroecology: the key role of arbuscular mycorrhizas in ecosystem services. Mycorrhiza 20, 519–30.

    Article  PubMed  Google Scholar 

  • Hossain MA, Nakano Y & Asada K, 1984. Monodehydroascorbate reductase in spinach chloroplasts and its participation in regeneration of ascorbate for scavenging hydrogen peroxide. Plant Cell Physiol 25, 385–395.

    CAS  Google Scholar 

  • Ijdo M, Cranenbrouck S & Declerck S, 2011. Methods for large-scale production of AM fungi: past, present, and future. Mycorrhiza 21, 1–16.

    Article  CAS  PubMed  Google Scholar 

  • Intra B, Mungsuntisuk I, Nihira T, Igarashi Y & Panbangred W, 2011. Identification of actinomycetes from plant rhizo-spheric soils with inhibitory activity against Colletotrichum spp., the causative agent of anthracnose disease. BMC Research Notes 4, 98.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Johansson JF, Paul LR & Finlay RD, 2004. Microbial interactions in the mycorrhizosphere and their significance for sustainable agriculture. FEMS Microbiol Ecol 48, 1–13.

    Article  CAS  PubMed  Google Scholar 

  • Klapheck S, Zimmer I & Cosse H, 1990. Scavenging of hydrogen peroxide in the endosperm of Ricinus communis by ascorbate peroxidase. Plant Cell Physiol 31, 1005–1013.

    CAS  Google Scholar 

  • Kormanik PP & McGraw AC, 1982. Quantification of vesicular-arbuscular mycorrhizae in plant roots. In: Schenck NC, ed. Methods and principles of mycorrhizal research. St Paul, MM, USA: American Phytopathological Society, 37–45.

    Google Scholar 

  • Knörzer OC, Durner J & Böger P, 1996. Alterations in the antioxidative system of suspension-cultured soybean cells (Glycine max) induced by oxidative stress. Physiol Plantarum 97, 388–396.

    Article  Google Scholar 

  • Kühn H & Borchert A, 2002. Regulation of enzymatic lipid peroxidation: The interplay of peroxidizing and peroxide reducing enzymes. Free Radic Biol Med 33, 154–172.

    Article  PubMed  Google Scholar 

  • Kuzniak E, Patykowski J & Urbanek H, 1999. Involvement of the antioxidative system in tomato response to fusaric acid treatment. J Phytopathol 147, 385–390.

    Article  CAS  Google Scholar 

  • Malenčić D, Kiprovski B, Popović M, Prvulović D, Miladinović J & Djordjević V, 2010. Changes in antioxidant systems in soybean as affected by Sclerotinia sclerotiorum (Lib.) de Bary. Plant Physiol Biochem 48, 903–908.

    Article  PubMed  Google Scholar 

  • McGonigle TP, Miller MH, Evans DG, Fairchild GL & Svan JA, 1990. A new method which gives an objective measure of colonization of roots by vesicular-arbuscular mycorrhizal fungi. New Phytol 115, 495–501.

    Article  Google Scholar 

  • Maiti D, Toppo NN & Variar M, 2011. Integration of crop rotation and arbuscular mycorrhiza (AM) inoculum application for enhancing AM activity to improve phosphorus nutrition and yield of upland rice (Oryza sativa L.) Mycorrhiza, ahead of print.

    Google Scholar 

  • Mauch F & Dudler R, 1993. Differential induction of distinct glutathione-S-transferase of wheat by xenobiotics and by pathogen attack. Plant Physiol 102, 1193–1201.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Mittova V, Guy M, Tal M & Volokita M, 2004. Salinity up-regulates the antioxidative system in root mitochondria and peroxisomes of the wild salt-tolerant tomato species Lycopersicon pennellii. J Exp Bot 55, 1105–1113.

    Article  CAS  PubMed  Google Scholar 

  • Oehl F, Sieverding E, P, Dubois D, Ineichen K, Boller T & Wiemken A, 2004. Impact of long-term conventional and organic farming on the diversity of arbuscular mycorrhizal fungi. Oecologia 138, 574–583.

    Article  PubMed  Google Scholar 

  • Patykowski J & Urbanek H, 2003. Activity of enzymes related to H2O2 generation and metabolism in leaf apoplastic fraction of tomato leaves infected with Botrytis cinerea. J Phytopathol 151, 153–161.

    Article  CAS  Google Scholar 

  • Peever L & Higgins V, 1989. Electrolyte leakage, lipoxygenase and lipid peroxidation induced in tomato leaf tissue by specific and non-specific elicitors of Cladosporoium fulvum. Plant Physiol 90, 867–875.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Phippen WB & Simon JE, 2000: Anthocyanin inheritance and instability in purple basil (Ocimum basilicum L.). J Hered 91, 289–296.

    Article  CAS  PubMed  Google Scholar 

  • Sekman AH, Türkan I & Takio S, 2007. Physiol Plant 131, 399–411.

    Article  Google Scholar 

  • Sheng M, Tang M, Chen H, Yang B, Zhang F & Huang F, 2008. Influence of arbuscular mycorrhizae on photosynthesis and water status of maize plants under salt stress. Mycorrhiza 18, 287–296.

    Article  CAS  PubMed  Google Scholar 

  • Smith S & Smith FA, 2011. Roles of arbuscular mycorrhizas in plant nutrition and growth: new paradigms from cellular to ecosystem scales. Annu Rev Plant Biol 62, 227–250.

    Article  CAS  PubMed  Google Scholar 

  • Toussaint J-P, Smith FA & Smith SE, 2007. Arbuscular mycorrhizal fungi can induce the production of phytochemicals in sweet basil irrespective of phosphorus nutrition. Mycorrhiza 17, 291–297.

    Article  CAS  PubMed  Google Scholar 

  • Toussaint J-P, Kraml M, Neil M, Smith SE, Smith FA, Steinkellner C, Schmiderer H, Vierheilig H & Novak J, 2008. Effect of Glomus mosseae on concentrations of rosmarinic and caffeic acids and essential compounds in basil inoculated with Fusarium oxysporum f.sp. basilica. Plant Pathol 57, 1109–1116.

    Article  Google Scholar 

  • Vanacker H, Carver TLW & Foyer CH, 1998. Pathogen-induced changes in the antioxidant status of the apoplast in barley leaves. Plant Physiol 117, 1103–1114.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Wu YX & Tiedemann von A, 2002. Evidence for oxidative stress involved in physiological leaf spot formation in winter and spring barley. Phytopathology 92, 145–155.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Center of Life Sciences, Institute of Bioanalytical Sciences (IBAS), Anhalt University of Applied Sciences, Strenzfelder Allee 28, 06406, Bernburg, Germany

    Jana Richter, Kathrin Kabrodt & Ingo Schellenberg

  2. Institute for Phytopathology and Applied Zoology (IPAZ), Justus-Liebig-University Gießen, Heinrich-Buff-Ring 22-26, Germany

    Helmut Baltruschat

Authors
  1. Jana Richter
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Helmut Baltruschat
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Kathrin Kabrodt
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ingo Schellenberg
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Helmut Baltruschat.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Richter, J., Baltruschat, H., Kabrodt, K. et al. Impact of arbuscular mycorrhiza on the St. John’s wort (Hypericum perforatum) wilt disease induced by Colletotrichum cf. gloeosporioides. J Plant Dis Prot 118, 109–118 (2011). https://doi.org/10.1007/BF03356390

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF03356390

Keywords

Search

Navigation