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Detection of Leptosphaeria maculans from imported Canola seeds

Nachweis von Leptosphaeria maculans in importiertem Rapssaatgut

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Abstract

Leptosphaeria maculans, the causal agent of blackleg on oilseeds in many regions around the world, has not been reported in China. In this study, L. maculans was detected from imported canola seeds by polymerase chain reaction (PCR) using a species-specific primer pair. The isolated L. maculans exhibited morphology identical to the known L. maculans strain on PDA culture plates and produced typical blackleg symptom when inoculated on oilseed leaves. It is the first report on the detection and identification of L. maculans from Canadian oilseed in China.

Zusammenfassung

Leptosphaeria maculans, der Erreger der Wurzelhals- oder Stängelfäule an Raps in vielen Regionen der Erde, wurde bisher in China nicht diagnostiziert. In unserer Untersuchung konnte L. maculans mit Hilfe der Polymerase-Kettenreaktion und einem artspezifischen Primerpaar in importierten Rapssamen nachgewiesen werden. Das L.-maculans-Isolat war auf Kartoffel-Glucose-Agar morphologisch mit einem bekannten L.-maculans-Isolat identisch und entwickelte nach einer Inokulation von Rapsblättern die typischen symptome der Wurzelhals- oder Stängelfäule. Dies ist der erste Bericht über die Entdeckung und Identifizierung von L. maculans in aus Kanada importiertem Raps in China.

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Authors and Affiliations

  1. Jiangsu Province Key Laboratory for Prevention and Management of Invasive Species, Nanjing Forest University, Nanjing, China

    G. Y. Chen & C. P. Wu

  2. Plant Quarantine Laboratory, Jiangsu Entry-Exit Inspection and Quarantine Bureau, Nanjing, China

    B. Li, H. Su, S. Z. Zhen & Y. L. An

Authors
  1. G. Y. Chen
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  2. C. P. Wu
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  3. B. Li
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  4. H. Su
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  5. S. Z. Zhen
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  6. Y. L. An
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Correspondence to C. P. Wu.

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Chen, G.Y., Wu, C.P., Li, B. et al. Detection of Leptosphaeria maculans from imported Canola seeds. J Plant Dis Prot 117, 173–176 (2010). https://doi.org/10.1007/BF03356356

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  • DOI: https://doi.org/10.1007/BF03356356

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