Abstract
Lanzhou lily (Lilium davidii var. unicolor) with symptoms of severe fasciation or dwarfing was observed in the central region of Gansu province, and the disease caused a serious loss in production of the bulb edible crop. In order to determine possible virus infections involved in the described disease, a set of enzyme-linked immunosorbent assays and immunoelectron microscopy had been performed to test the naturally infected plants. A mixed infection which includes Lily symptomless virus (LSV: Flexiviridae, Carlavirus), Cucumber mosaic virus (CMV: Bromoviridae, Cucumovirus) and Lily mottle virus (LMoV: Potyviridae, Potyvirus) has been confirmed by serologic and morphologic evidences. The subsequent thin sectioning observation with a transmission electron microscope on different tissues from infected samples showed that cytopathologic damages happened. Total RNA extracted from different tissues of infected plants was used in the Northern-blotting assays with probes of coat protein gene of each virus, then hybridization data showed a symptomatic infection of related virus. Thus, the study gives reliable serologic, morphologic, cytopathologic and molecular evidences for the diagnosis of the systemic infections. This study is hoped to be useful in prevention of certain viral diseases and to increase the quality and production of this crop in the future.
Zusammenfassung
Lanzhou-Lilien (Lilium davidii var. unicolor) mit deutlichen Verbänderungs- und Verzwergungssymptomen wurden im Herzen der nordwestchinesischen Provinz Gansu beobachtet. Die Erkrankung verursachte schwere Ertragsverluste beim Anbau der essbaren Lilienzwiebeln. Zur Diagnose möglicher Virusinfektionen wurden verschiedene enzymgekoppelte Immunadsorptionstests (ELISA) und Immunelektronenmikroskopie an natürlich infizierten Pflanzen durchgeführt. Eine Mischinfektion mit dem Symptomlosen Lilienvirus (Lily symptomless virus, LSV: Flexiviridae, Carlavirus), Gurkenmosaikvirus (Cucumber mosaic virus, CMV: Bromoviridae, Cucumovirus) und dem Lilienfleckenvirus (Lily mottle virus, LMoV: Potyviridae, Potyvirus) wurde serologisch und morphologisch bestätigt. Die transmissionselektronenmikroskopische Untersuchung von Gewebe-Dünnschnitten zeigte cytopathologische Veränderungen. Die Gesamt-RNA verschiedener Gewebe infizierter Pflanzen konnte im Northern-Blot mit den Hüllproteingenen der drei Viren hybridisiert werden, was die Präsenz der Viren in den entsprechenden Pflanzengeweben bestätigte. Die Untersuchung ergab daher zuverlässige serologische, morphologische, cytopathologische und molekulare Bestätigungen für die Diagnose systemischer Virusinfektionen. Die Untersuchung könnte hilfreich bei der Entwicklung von Bekämpfungsverfahren gegen diese Pflanzenviren sein und den qualitativen und quantitativen Ertrag der Lanzhou-Lilien zukünftig steigern.
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Wang, R., Wang, G., Zhao, Q. et al. Molecular and cytopathologic evidences for a mixed infection of multiple viruses on Lanzhou lily (Lilium davidii var. unicolor) in Northwestern China. J Plant Dis Prot 117, 145–149 (2010). https://doi.org/10.1007/BF03356351
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DOI: https://doi.org/10.1007/BF03356351