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Journal of Plant Diseases and Protection

, Volume 117, Issue 3, pp 97–101 | Cite as

Effect of the disodium salt of cyclopentene β,β′-triketone on the development of infections induced by TMV in the leaves of hypersensitive and susceptible tobacco plants

  • A. Reunov
  • L. Lapshina
  • V. Nagorskaya
  • O. Shestak
  • V. Novikov
Article
  • 5 Downloads

Abstract

We show here that the disodium salt of 2-acetyl-5-chloro-4- hydroxycarbonylmethylthiocyclopent-4-ene-1,3-dione (salt 2) inhibits tobacco mosaic virus (TMV)-induced infections in detached leaves of TMV-resistant (Xanthi-nc) and -susceptible (Samsun) varieties of Nicotiana tabacum L. Inoculation of Xanthi-nc leaves with TMV mixed with salt 2 (2 mg/ml) resulted in a significant decrease (78%) in the number of local lesions as compared to leaves inoculated with TMV alone. By applying the salt immediately before or after infection, the inhibitory effect on the number of lesions decreased. The infectivity and content of viral antigen in Samsun leaves inoculated with a mixture of TMV and salt 2 were considerably less early in infection compared to leaves inoculated with TMV only. As the infection progressed, the inhibitory action of salt 2 decreased. Applying the salt 24 h before infection enhanced the number of both local and heat-induced lesions in Xanthi-nc and Samsun leaves, respectively, that is increased the number of “infectious centers” produced on the leaf surface, which probably promoted virus penetration into the host cells. At the same time, pretreatment decreased the infectivity in Xanthi-nc leaf lesions and infectivity and viral antigen accumulation in Samsun leaves. Therefore, the antiviral effects of salt 2 are not because of diminished penetration of the virus into cells, but seem to be caused by salt 2-mediated inhibition of virus replication.

Key words

antiviral activity Nicotiana tabacum disodium salt of 2-acetyl-5-chloro-4-hydroxycarbonylmethylthiocyclopent-4-ene-1,3-dione tobacco mosaic virus 

Einfluss des Dinatrium-Salzes von Cyclopenten-ß-ß′-Triketon auf die Infektion hypersensitiver und anfälliger Tabakblätter mit TMV

Zusammenfassung

Wir können in dieser Untersuchung zeigen, dass das Dinatrium- Salz von 2-Acetyl-5-chlor-4-hydroxycarbonylmethylthiocyclopent- 4-en-1,3-dion (Salz 2) die Infektion abgeschnittener Blätter Tabakmosaikvirus (TMV)-resistenter (Xanthi-nc) und -anfälliger (Samsun) Sorten von Nicotiana tabacum L. durch das TMV hemmt. Die Inokulation von Xanthi-nc-Blättern mit einer Mischung von TMV und dem Salz 2 (2 mg ml–1) resultierte in einer signifikanten Verminderung (78%) lokaler Blattläsionen im Vergleich zu nur mit dem Virus inokulierten Blättern. Bei Anwendung des Salzes unmittelbar vor oder nach einer Infektion verminderte sich die hemmende Wirkung auf die Anzahl der Läsionen wieder. Die Infektiösität und der Gehalt an viralen Antigenen in mit einer Mischung von TMV und dem Salz 2 inokulierten Blättern der anfälligen Sorte Samsun waren im Vergleich mit nur mit dem Virus inokulierte Blättern deutlich reduziert. Im Verlauf einer fortschreitenden Infektion nahm die Hemmwirkung des Salzes 2 ab. Die Anwendung des Salzes 24 h vor einer TMV-Infektion erhöhte die Anzahl lokaler und hitzeinduzierter Blattläsionen in Xanthi-nc und Samsun. Die Anzahl dieser auf der Blattoberfläche gebildeten „Infektionszentren” förderte wohl das Eindringen der Viren in die Pflanzenzellen. Zum selben Zeitpunkt verminderte eine Vorbehandlung die Infektiosität in Xanthi-nc-Blattläsionen und sowohl die Infektiosität als auch die Akkumulation viraler Antigene in Samsun-Blättern. Die antiviralen Effekte des Salzes 2 resultieren daher nicht aus einem verminderten Eindringen des Virus in die Zellen, sondern scheinbar aus einer Salz-2-beeinflussten Störung der Virusreplikation.

Stichwörter

2-Acetyl-5-chlor-4-hydroxycarbonylmethylthiocyclopent-4-en-1,3-dion-Dinatrium-Salz antivirale Aktivität Nicotiana tabacum Tabakmosaikvirus 

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Copyright information

© Deutsche Phythomedizinische Gesellschaft 2010

Authors and Affiliations

  • A. Reunov
    • 1
  • L. Lapshina
    • 1
  • V. Nagorskaya
    • 1
  • O. Shestak
    • 1
  • V. Novikov
    • 1
  1. 1.Pacific Institute of Bioorganic ChemistryFar East Branch of Russian Academy of SciencesVladivostokRussia

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