Abstract
Changes of malondialdehyde (MDA) content, the activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) in opium poppy, Papaver somniferum were measured at the bud stage treated with different concentrations of paraquat. The results showed that the changing rate of MDA content was the same as that of the cell membrane electrolyte leakage. The MDA content and cell membrane electrolyte leakage decreased in the early stage then increased under 3 ml l–1, 6 ml l–1, 12 ml l–1 treatment. In contrast, the MDA content and relative membrane permeability increased in the early stage then decreased under 24 ml l–1 treatment. Antiox-idant enzyme activities varied under different treatments. SOD activities decreased during 3–24 h and then increased under the 24 ml l–1 treatment. All the peaks of POD activities appeared at the 3 h post treatment. POD activities at the 9 h and the 36 h under 24 ml l–1 treatment were significantly lower than in the control. CAT activities varied under different treatments, increased at 9 h and decreased at 36 h post treatment under 24 ml l–1 treatment. This means that the active oxygen produced in the leaf cells of opium poppy was beyond the recovering capacity of antioxidant enzyme, resulting in the damage of enzyme. These results suggest that a high concentration (24 ml l–1) paraquat treatment caused damages by speeding up the peroxide of the opium poppy plant cells, whereas lower concentrations were less effective.
Zusammenfassung
Die Veränderung des Malondialdehydgehalts (MDA) sowie der Aktivitäten der Superoxiddismutase (SOD), Peroxidase (POD) und Katalase (CAT) wurden im Knospenstadium des Schlaf-Mohns (Papaver somniferum) nach Behandlung mit verschiedenen Paraquatkonzentrationen bestimmt. Die Ergebnisse zeigen, dass die Veränderung des MDA-Gehalts mit dem Elektrolytverlust der Pflanzenzellen korrelierte. Beide Werte sanken zu Anfang, stiegen dann aber wieder nach den Behandlungen mit 3 ml l–1, 6 ml l–1 und 12 ml l–1 Paraquat. Im Gegensatz dazu nahmen der MDA-Gehalt und die relative Membranpermeabilität anfangs zu, sanken dann aber nach einer Behandlung mit 24 ml l–1 Paraquat. Die Aktivitäten antioxidativer Enzyme variierten je nach Behandlung. Die SOD-Aktivität sank nach einer Behandlung mit 24 ml l–1 Paraquat während der ersten 24 h und stieg dann wieder an. Die höchste POD-Aktivität wurde 3 h nach der Behandlung gemessen. Die POD-Aktivität war 9 und 36 h nach der Behandlung mit 24 ml l–1 signifikant geringer als in der Kontrolle. Die CAT-Aktivität stieg bis 9 h nach der Behandlung mit 24 ml l–1 und sank danach bis zum Ende der Messung nach 36 h. Dies bedeutet, dass die Menge der in den Blattzellen des Schlaf-Mohns gebildeten reaktiven Sauerstoffspezies die Schutzwirkung der antioxidativen Enzyme übertraf, woraus Enzym-schädigungen resultierten. Die Ergebnisse deuten darauf hin, dass eine Behandlung mit einer hohen Paraquatkonzentration (24 ml l–1) durch die Erhöhung der Peroxidbildung pflanzenschädigend wirkt, während geringere Konzentrationen weniger wirksam sind.
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Zhao, R., Guo, P.Y., Yuan, X.Y. et al. Effect of paraquat on the antioxidative enzyme activities and lipid peroxidation in opium poppy (Papaver somniferum L.). J Plant Dis Prot 117, 55–59 (2010). https://doi.org/10.1007/BF03356335
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DOI: https://doi.org/10.1007/BF03356335
Key words
- active oxygen
- catalase (CAT)
- malondialdehyde (MDA)
- peroxidase (POD)
- relative membrane permeability
- superoxide dismutase (SOD)