Abstract
Mixtures of micro-organisms may lead to more effective control of plant pathogens than biocontrol with single species, but little data exists on the efficacy of microbial combinations against powdery mildews. In a field experiment in northern California with high disease pressure, we found, at best, only modest disease control with individual use of three species of commercially-available bacterial antagonists. Application of all species as a mixture or in rotation did not improve disease control more than the best monoculture, suggesting a lack of synergism among mixture components.
Zusammenfassung
Die kombinierte Anwendung verschiedener antagonistischer Mikroorganismen kann höhere Wirkungsgrade gegenüber Pflanzenpathogenen erreichen als Einzelpräparate. Bisher sind nur wenige Daten zur Wirkung biologischer Kombinationspräparate gegenüber Echten Mehltaupilzen verfügbar. Ein Feldversuch an einem nordkalifornischen Standort mit hohem Infektionspotential ergab nur mäßige Bekämpfungserfolge mit drei kommerziell vertriebenen mikrobiellen Präparaten gegenüber dem Echten Gurkenmehltau an Kürbis. Die kombinierte oder aufeinander folgende Anwendung der drei Präparate zeigte keine höheren Wirkungsgrade als die ertragreichste Monokulturvariante, was auf das Fehlen einer synergistischen Wirkung der mikrobiellen Mischungspartner hindeutet.
References
Andrews, J.H., 1992: Biological control in the phyllosphere. Annu. Rev. Phytopathol. 30, 603–635.
Elad, Y., B. Kirshner, N. Yehuda, A. Sztejnberg, 1998: Management of powdery mildew and grey mold of cucumber by Trichoderma harzianum T39 and Ampelomyces quisqualis AQ10. Biocontrol 43, 241–251.
Gilardi, G., D.C. Manker, A. Garibaldi, M.L. Gullino, 2008: Efficacy of the biocontrol agents Bacillus subtilis and Ampelomyces quisqualis applied in combination with fungicides against powdery mildew of zucchini. J. Plant Dis. Protect. 115, 208–213.
Guetsky, R., D. Shteinberg, Y. Elad, A. Dinoor, 2001: Combining biocontrol agents to reduce the variability of biological control. Phytopathology 91, 621–627.
Haggag, W.M., M.A. Nofal, 2006: Improving the biological control of Botryodiplodia disease on some Annona cultivars using single or multi-bioagents in Egypt. Biol. Control 38, 341–349.
Jahn, M., H.M. Munger, J.D. Mccreight, 2002: Breeding cucurbit crops for powdery mildew resistance. In: R.R. Bélanger, W.R. Bushnell, A.J. Dik, T.L.W. Carter (eds.): The Powdery Mildews. A Comprehensive Treatise, pp. 239–248. APS Press, St. Paul, MN, USA.
Jetiyanon, K., J.W. Kloepper, 2002: Mixtures of plant growth-promoting rhizobacteria for induction of systemic resistance against multiple plant diseases. Biol. Control 24, 285–291.
Joshi, R., B.B. Mcspadden Gardener, 2006: Identification and characterization of novel genetic markers associated with biological control activities in Bacillus subtilis. Phytopathology 96, 145–154.
Kiss, L., 2003: A review of fungal antagonists of powdery mildews and their potential as biocontrol agents. Pest Manage. Sci. 59, 475–483.
Koch, E., 1999: Evaluation of commercial products for microbial control of soil-borne plant diseases. Crop Prot. 18, 199–125.
Lemanceau, P., P.A.H.M. Bakker, W.J. de Kogel, C. Alabouvette, Schippers, 1992: Effect of pseudobactin 358 production by Pseudomonas putida WCS358 on suppression of fusarium wilt of carnations by nonpathogenic Fusarium oxysporum Fo47. Appl. Environ. Microbiol. 58, 2978–2982.
Ojiambo, P.S., H. Scherm, 2006: Biological and application-oriented factors influencing plant disease suppression by biological control: a meta-analytical review. Phytopathology 96, 1168–1174.
Roberts, D.P., S.M. Lohrke, S.L.F. Meyer, J.S. Buyer, J.H. Bowers, C.J. Baker, W. Li, J.T. de Souza, J.A. Lewis, S. Chung, 2005: Biocontrol agents applied individually and in combination for suppression of soilborne diseases of cucumber. Crop Prot. 24, 141–155.
Thrane, C., D.F. Jensen, A. Tronsmo, 2000: Substrate colonization, strain competition, enzyme production in vitro, and biocontrol of Pythium ultimum by Trichoderma spp. isolates P1 and P3. Eur. J. Plant Pathol. 106, 215–225.
Tosi, L., A. Zazzerini, 1994: Evaluation of some fungi and bacteria for potential control of safflower rust. J. Phytopathol. 142, 131–140.
Vanneste, J.L., 1996: Honey bees and epiphytic bacteria to control fire blight, a bacterial disease of apple and pear. Biocontrol News Inf. 17, 67N–78N.
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Janousek, C.N., Lorber, J.D. & Gubler, W.D. Combination and rotation of bacterial antagonists to control powdery mildew on pumpkin. J Plant Dis Prot 116, 260–262 (2009). https://doi.org/10.1007/BF03356320
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DOI: https://doi.org/10.1007/BF03356320