Skip to main content
SpringerLink
Log in

Influence of Fusarium wilt resistant tomato cultivars on root colonization of the mutualistic endophyte Fusarium oxysporum strain 162 and its biological control efficacy toward the root-knot nematode Meloidogyne incognita

Einfluss von gegenüber der Fusariumwelke resistenten Tomatensorten auf die Wurzelbesiedlung durch den mutualistischen Endophyten Fusarium oxysporum 162 und dessen biologische Kontrolle des Wurzelgallennematoden Meloidogyne incognita

  • Published:
Journal of Plant Diseases and Protection Aims and scope Submit manuscript

Abstract

The ability of the nematode antagonistic, mutualistic endo-phyte Fusarium oxysporum strain 162, to colonize the endorhiza of different Fusarium wilt resistant and susceptible cultivars of tomato (Solanum lycopersicon L.) was investigated. The fungus colonized the endorhiza of both wilt resistant and susceptible cultivars of tomato. Colonization after 3 weeks ranged from 40% to 100% in trial 1 and from 32% to 75% in trial 2. Percent colonization declined 6 weeks after fungal inoculation in resistant as well as susceptible varieties. Biocontrol presented by nematode penetration in trial 1 and number of galls in trial 2 reached 82% and 55%, respectively. Surprisingly, the level of nematode control in Fusarium wilt resistant cultivars was found similar or even higher in some cases when compared to that in wilt susceptible cultivars. Although endophyte colonization decreased over time, the antagonistic fungus reduced Meloidogyne incognita infestation significantly in the majority of the cultivars tested.

Zusammenfassung

Ziel der Untersuchung war es festzustellen ob der gegen Nematoden wirkenden mutualistischen Endophyten Fusa-rium oxysporum 162 fähig ist, verschiedene gegenüber Fusariumwelke resistenter bzw. anfälige Tomatensorten (Solanum lycopersicon) zu besiedeln. Der Pilz besiedelte die Endorhiza sowohl der resistenten wie auch die der anfälligen Tomatensorten. Nach drei Wochen die Besiedlungsrate zwischen 40% und 100% in Versuch 1 bzw. zwischen 32% und 75% in Versuch 2. Der Anteil besiedelter Pflanzen nahm sechs Wochen nach der Pilzinokulation sowohl bei resistenten als auch anfäligen Sorten ab. Die biologische Bekämpfung bewirkte in den beiden Experimenten eine Reduzierung der Eindringung um 82% bzw. der Gallenbildung um 55%. überraschenderweise war in einigen Fällen die Nematoden-bekämpfung bei den resistenten Sorten vergleichbar oder sogar höher als bei den anfälligen Sorten. Obwohl die Besiedlung durch den Endophyten im Verlauf der Zeit abnahm, reduzierte der antagonistische Pilz signifikant den Befall durch M. incognita bei der Mehrzahl der getesteten Tomatensorten.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Literature

  • Alabouvette, C., P. Lemanceau, C. Steinberg, 1993: Recent advances in the biological control of Fusarium wilt. Pestic. Sci. 37, 365–373.

    Article  Google Scholar 

  • Alabouvette, C., V. Edel, P. Lemanceau, C. Olivain, G. Recorbet, C. Steinberg, 2001: Diversity and interactions among strains of Fusarium oxysporum: Application and biological control. In: M.J. Jeger, N.J. Spence (eds.): Biotic Interactions in Plant-pathogen Associations, pp 131–157. CAB International, Wallingford, United Kingdom.

    Chapter  Google Scholar 

  • Armstrong, G.M., J.K. Armstrong, 1981: Formae speciales and races of Fusarium oxysporum causing wilt diseases. In: P.E. Nelson, T.A. Toussoun, R.J. Cook (eds.) Fusarium: Diseases, Bbiology, and Taxonomy, pp. 391–399. Pennsylvania State University Press, University Park and London.

    Google Scholar 

  • Dababat, A.A., R.A. Sikora, 2007a: Induced resistance by the mutualistic endophyte, Fusarium oxysporum 162, toward Meloidogyne incognita on tomato. Biocontrol Sci. Techn. 17, 969–975.

    Article  Google Scholar 

  • Dababat, A.A., R.A. Sikora, 2007b: Influence of the mutualistic endophyte Fusarium oxysporum 162 on Meloidogyne incognita attraction and invasion. Nematology 9, 771–776.

    Article  Google Scholar 

  • Dababat, A.A., R.A. Sikora, 2007c: Importance of application time and inoculum density of the non-pathogenic endophytic fungus, Fusarium oxysporum 162, for the biological control of the root-knot nematode Meloidogyne incognita on tomato. Nematropica 2, 267–276.

    Google Scholar 

  • Duijff, B.J., D. Pouhair, C. Olivain, C. Alabouvette, P. Lemanceau, 1998: Implication of systemic induced resistance in the suppression of Fusarium wilt of tomato by Pseudomo-nas flourescens Wcs417r and by non-pathogenic Fusarium oxysporum Fo47. Eur. J. Plant Pathol. 104, 903–910.

    Article  Google Scholar 

  • Eparvier, A., C. Alabouvette, 1994: Use of ELISA and GUS-transformed strains to study competition between pathogenic and non-pathogenic Fusarium oxysporum for root colonization. Biocontrol Sci. Techn. 4, 35–47.

    Article  Google Scholar 

  • Gordon, T.R., D. Okamoto, 1992: Population structure and the relationship between pathogenic and non-pathogenic strains of Fusarium oxysporum. Phytopathology 82, 73–77.

    Article  Google Scholar 

  • Hallmann, J., R.A. Sikora, 1994a: Occurrence of plant parasitic nematodes and nonpathogenic species of Fusarium in tomato plants in Kenya and their role as mutualistic syner-gists for biological control of root knot nematodes. Int. J. Pest Manage. 40, 321–325.

    Article  Google Scholar 

  • Hallmann, J., R.A. Sikora, 1994b: Influence of Fusarium oxysporum, a mutualistic fungal endophyte, on Meloidogyne incognita of tomato. J. Plant Dis. Protect. 101, 475–481.

    Google Scholar 

  • Handelsman, J., E.V. Stabb, 1996: Biocontrol of soilborne plant pathogens. Plant Cell 8, 1855–1869.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Hussey, R.S., K.R. Barker, 1973: A comparison of methods of collecting inocula of Meloidogyne spp., including a new technique. Plant Dis. Rep. 57, 1025–1028.

    Google Scholar 

  • Kimenju, J.W., S.W. Wando, A.W. Mwang’ombe, R.A. Sikora, R.P. Schurter, 1998: Distribution of lesion nematodes associated with maize in Kenya and susceptibility of maize cultivars to Pratylenchus zeae. Afr. Crop Sci. J. 6, 367–375.

    Google Scholar 

  • Larkin, R.P., D.L. Hopkins, F.N. Martin, 1996: Suppression of Fusarium wilt of watermelon by non-pathogenic Fusarium oxysporum and other microorganisms recovered from disease suppressive soil. Phytopathology 86, 812–819.

    Google Scholar 

  • Mandeel, Q., R. Baker, 1991: Mechanisms involved in biological control of Fusarium wilt of cucumber with strains of non-pathogenic Fusarium oxysporum. Phytopathology 81, 462–469.

    Article  Google Scholar 

  • Niere, B.I., P.R. Speijer, C.S. Gold, R.A. Sikora, 1998: Fungal endophytes from banana for the biocontrol of Radopholus similis. In: E.A. Frison, C.S. Gold, E.B. Karamura, R.A. Sikora (eds.): Mobilizing IPM for Sustainable Production in Africa. Proceedings of a workshop on banana IpM held in Nelspruit, South Africa, 23–28 Nov. 1998: INIBAP, pp. 313–318. Montpellier, France.

    Google Scholar 

  • Niere, B.I., 2001: Significance of non-pathogenic isolates of Fusarium oxysporum Schlecht: Fries for the biological control of the burrowing nematode Radopholus similis (Cobb) Thorne on tissue cultured banana. PhD thesis, University of Bonn.

    Google Scholar 

  • Niere, B.I., R.A. Sikora, P.R. Speijer, 2001: Mutualistic endo-phytic fungirole in biocontrol and safety of application. In: R.A. Sikora (ed): Integrated Control of Soil Pests. IOBC/WPRS Bull. 24, 117–120.

    Google Scholar 

  • Olivain, C., C. Alabouvette, 1997: Colonization of tomato root by a non-pathogenic strain of Fusarium oxysporum. New Phytol. 137, 481–494.

    Article  Google Scholar 

  • Olivain, C., C. Alabouvette, 1999: Process of tomato root colonization by a pathogenic strain of Fusarium oxysporum f. sp. lycopersici in comparison with a non-pathogenic strain. New Phytol. 141, 497–510.

    Article  Google Scholar 

  • Olivain, C., S. Trouvelot, M. Binet, C. Cordier, A. Pugin, C. Alabouvette, 2003: Colonization of flax roots and early physiological responses of flax cells inoculated with pathogenic and non-pathogenic strains of Fusarium oxysporum. Appl. Environ. Microbiol. 69, 5453–5462.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Olivain, C., C. Humbert, J. Nahalkova, J. Fatehi, F. L’haridon, C. Alabouvette, 2006: Colonization of tomato by pathogenic and non-pathogenic Fusarium oxysporum strains inoculated together and separately into the soil. Appl. Environ. Microbiol. 72, 1–9.

    Article  Google Scholar 

  • Pocasangre, L.E., R.A. Sikora, V. Vilich, R.-P. Schuster, 2000: Survey of banana endophytic fungi from Central America and screening for biological control of Radopholus similis. In: M. Blanke, J. Pohlan (eds.): Proceedings of the 2nd ISHS Conference on Fruit production in the Tropics and Subtropics, held on 24–26 June 1999, pp. 283–289. Bonn, Germany.

    Google Scholar 

  • Sas Institute Inc., 2005: SaS 9.1 User’s Guide. SAS Institute Inc., Cary, NC, USA.

    Google Scholar 

  • Sikora, R.A., R.-P. Schuster, 2000: Handbuch der Phytonema-tologie. Shaker Verlag, Aachen, Germany.

    Google Scholar 

  • Speijer, P.R., 1993: Interrelationship between Pratylenchus goodeyi Sher & Allen and strains of non-pathogenic Fusarium oxysporum Schl. emd. Snyd. & Hans. in roots of banana cultivars. PhD thesis, University of Bonn.

    Google Scholar 

  • Vu, T.T., 2005: Mode of action of non-pathogenic Fusarium oxysporum endophytes for bio-enhancement of banana toward Radopholus similis. PhD thesis, University of Bonn.

    Google Scholar 

  • Vu, T. T., R. Hauschild, R.A. Sikora, 2006: Fusarium oxysporum endophytes induced systemic resistance against Radopholus similis on banana. Nematology 8, 847–852.

    Article  Google Scholar 

  • zum Felde, A., A.L. Pocasangre, R.A. Sikora, 2005: The potential use of microbial communities inside suppressive banana plants for banana root protection. In: D.W. Turner, F.E. Rosales (eds.): Banana Root System: Towards a Better Understanding for its Productive Management. Proceedings of an international symposium. International Network for the Improvement of Banana and Plantain (Inibap), pp. 169–177. Montpellier, France.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Soil-Ecosystem Phytopathology and Nematology, Institute of Crop Science and Resource Conservation, Department of Plant Health, University of Bonn, Nussallee 9, 53115, Bonn, Germany

    A. A. Dababat, M. E. Selim & R. A. Sikora

  2. Department of Biological Sciences, Faculty of Science and Technology, Al-Quds University, Abu Deis, P.O. Box. 20002, Jerusalem

    A. A. Saleh

Authors
  1. A. A. Dababat
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. E. Selim
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. A. Saleh
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. R. A. Sikora
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to R. A. Sikora.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Dababat, A.A., Selim, M.E., Saleh, A.A. et al. Influence of Fusarium wilt resistant tomato cultivars on root colonization of the mutualistic endophyte Fusarium oxysporum strain 162 and its biological control efficacy toward the root-knot nematode Meloidogyne incognita. J Plant Dis Prot 115, 273–278 (2008). https://doi.org/10.1007/BF03356276

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF03356276

Key words

Stichwörter

Search

Navigation