Abstract
Sixty-three isolates of Didymella rabiei collected from southern Alberta were analyzed for pathogenic variability using six differential chickpea genotypes. Although all isolates were pathogenic on all of the differentials, they varied in virulence. The reaction of host genotypes was classified as resistant or susceptible based on the severity of disease symptoms, and isolates were grouped into 25 pathotypes, suggesting a very high level of diversity in the population of D. rabiei. The predominant pathotypes were XV and XVI, each comprising 14% of the total number of isolates tested. Pathotypes VII and XXV each represented 8% of the isolates tested, while patho-types III, IV and XIX each represented 6%. The rest of the pathotypes each represented 2–3% of the total isolates. Cluster analysis generated two main groups, at an average distance of 1.0 between the clusters. Fourteen subgroups were identified among the isolates at an average distance between isolates and within subgroups of 0.5. No associations were found between geographical origins, pathotypes, cluster groupings, parts of the plants or the host varieties from which the isolates were obtained.
Zusammenfassung
Die Pathogenitätsunterschiede von 63 Isolaten von Didymella rabiei aus dem Süden der kanadischen Provinz Alberta wurden an sechs Differentialgenotypen der Kichererbse untersucht. Obwohl alle Isolate an allen Genotypen pathogen waren, unterschieden sie sich in der Virulenz voneinander. Die Reaktion der Genotypen wurde auf Grundlage der ermittelten Befallsstärke als resistent oder anfällig klassifiziert. Die Pilzisolate wurden in 25 Pathotypen gruppiert, was auf eine sehr hohe genetische Diversität von D. rabiei hindeutet. Die vorherrschenden Pathotypen waren die Isolatgruppen XV und XVI mit jeweils 14% der untersuchten Isolate. Die Pathotypen VII und XXV umfassten je 8% aller Versuchsisolate, während die Pathotypen III, IV und XIX jeweils 6% der Isolate repräsentierten. Die verbleibenden Pathotypen umfassten jeweils zwischen 2 und 3% aller untersuchten Pilzisolate. Zwei Hauptgruppen mit einer mittleren Distanz von 1,0 voneinander wurden durch eine Clusteranalyse ermittelt. Die Isolate wurden in 14 Untergruppen mit einer mittleren Distanz von 0,5 zwischen ihnen und innerhalb der Untergruppen eingeteilt. Zwischen der geografischen Herkunft, den Pathotypen, Clustergruppen, Pflanzenteilen oder Wirtsgenotypen der Isolate konnte keine Beziehung ermittelt werden.
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Ahmed, H.U., Chang, K.F., Hwang, S.F. et al. Pathogenic diversity of Didymella rabiei isolates from southern Alberta, Canada. J Plant Dis Prot 114, 189–195 (2007). https://doi.org/10.1007/BF03356218
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DOI: https://doi.org/10.1007/BF03356218