Journal of Plant Diseases and Protection

, Volume 113, Issue 6, pp 275–282 | Cite as

Intraspecific Variation in isolates of Bursaphelenchus xylophilus (Nematoda: Aphelenchoididae) revealed by ISSR and RAPD fingerprints

  • K. Metge
  • W. Bürgermeister


The pinewood nematode Bursaphelenchus xylophilus is the causal agent of pine wilt disease. In order to trace the origin of its recently introduced Portuguese population, two PCR-based techniques, random amplified polymorphic DNA (RAPD) and inter-simple sequence repeats (ISSR), were used to determine genetic relationships among 30 B. xylophilus isolates from the Usa, Canada, Japan, China, South Korea and Portugal. Fingerprints obtained with both methods detected a reduced genetic variation of introduced popula- tions as compared to native North American populations. Cluster analyses of genetic distances between isolates were carried out and bootstrap dendrograms were constructed. The results indicated that founders of the Portuguese popula- tions most likely were translocated one or two times to Portugal from their colonized sites in East Asia, but not from their native habitats in North America.

Key words

genetic variation inter simple sequence repeat invasive alien species pathway of introduction pinewood nematode random amplified polymorphic DNA 

Bestimmung der intraspezifischen Variabilität von Isolaten des Kiefernholznematoden Bursaphelenchus xylophilus (Nematoda: Aphelenchoididae) mittels ISSR- und RAPD-Fingerprints


Der Kiefernholznematode Bursaphelenchus xylophilus ist für die sogenannte Kiefernwelke verantwortlich. Um die Herkunft der eingeschleppten portugiesischen Population zu bestimmen, wurden die genetischen Unterschiede von 30 B. xylophilus’-Herkünften aus den USA, Kanada, Japan, China, Südkorea und Portugal mit 2 PCR-basierten Techniken, RAPD (random amplified polymorphic DNA) und ISSR (inter-simple sequence repeats) untersucht. Die erhaltenen Fingerprints der Herkünfte zeigten mit beiden Methoden eine reduzierte Anzahl von potenziellen Markern für Isolate aus Einschleppungsgebieten im Vergleich zu Isolaten aus dem Ursprungsvorkommen in Nordamerika. Clusteranalysen der genetischen Distanzen der Herkünfte wurden ausgeführt und Dendro- gramme konstruiert. Die Ergebnisse weisen darauf hin, dass sich das portugiesische Vorkommen aus ein- bis zwei Einschleppungen von Tieren aus Ostasien etabliert haben kann. Es gründet sich demnach nicht auf Tiere aus Nordamerika.


gebietsfremde invasive Art genetische Variation Herkunftsanalyse ISSR Kiefernholznematode RAPD 


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Copyright information

© Deutsche Phythomedizinische Gesellschaft 2006

Authors and Affiliations

  1. 1.Institute for Plant Virology, Microbiology and BiosafetyFederal Biological Research Centre for Agriculture and Forestry (BBA)BraunschweigGermany

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