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Journal of Plant Diseases and Protection

, Volume 113, Issue 4, pp 168–173 | Cite as

Activity of four entomopathogenic nematode species against young adults of Sitophilus granarius (Coleoptera: Curculionidae) and Oryzaephilus surinamensis (Coleoptera: Silvanidae) under laboratory conditions

  • S. TrdanEmail author
  • M. Vidrih
  • N. Valic
Article

Abstract

Four entomopathogenic nematode species (Steinernema fel-tiae, Steinernema carpocapsae, Heterorhabditis bacteriophora, and Heterorhabditis megidis) were tested in a laboratory bioassay with the aim of studying their efficacy in control of the adults of two stored grain pests, Sitophilus granarius and Oryzaephilus surinamensis. Activity of the biological agents studied was determined at three different concentrations (500, 1000, and 2000 infective juveniles [IJs] per adult) and temperatures (15, 20, and 25°C). The granary weevil mortality rate was higher than the mortality rate of the saw-toothed grain beetle. Heterorhabditis megidis proved to be the least efficient in control of both pests, while no significant differences were recorded between any of the other three nematode species. The experiment demonstrated that the entomopathogenic nematodes were most efficient in the control of S. granarius at 20°C (LC50 after 7-day exposure 803–1195 IJs/adult) and 25°C (LC50 505–1175 IJs/adult). A satisfactory level in control of the pest O. surinamensis was reached at 20°C (LC50 921–1335 IJs/adult). The concentration of the suspension used in our experiment was shown to be a less important factor affecting the biological activity of nematodes against the adults of both stored grain pests. Though the use of entomopathogenic nematodes for control of the tested pests is not possible at the present time, it may be possible to combine this approach with some other (biotechnical) methods in the future.

Keywords

biological control efficacy entomopathogenic nematodes Oryzaephilus surinamensis Sitophilus granarius 

Wirkung von vier entomopathogenen Nematodenarten gegenüber Sitophilus granarius (Coleoptera: Curculionidae) und Oryzaephilus surinamensis (Coleoptera: Silvanidae) unter Laborbedingungen

Zusammenfassung

Vier entmopathogene Nematodenarten (Steinernema feltiae, Steinernema carpocapsae, Heterorhabditis bacteriophora und Heterorhabditis megidis) wurden in einem Labor-Biotest hinsichtlich ihrer Wirksamkeit bei der Bekämpfung zweier Vorratsschädlinge des Getreides, Sitophilus granarius und Oryzaephilus surinamensis, untersucht. Ihre Wirksamkeit wurde in drei verschiedenen Konzentrationen (500, 1000 und 2000 Infektionslarven [IL] pro Adultem) und Temperaturen (15, 20 und 25°C) ermittelt. Die Mortalität des Kornkäfers S. granarius übertraf dabei die des Getreideplattkäfers O. surinamensis. Heterorhabditis megidis war die ineffektivste Nematodenart gegenüber beiden Vorratsschädlingen, während zwischen den drei anderen Nematodenarten keine signifikanten Unterschiede auftraten. Das Experiment zeigt, dass die entomopa-thogenen Nematoden den Kornkäfer am effektivsten bei Temperaturen von 20°C (LC50 nach 7 Tagen 803–1195 IL/Adultem) und 25°C (LC50 505–1175 IL/Adultem) kontrollieren. Ein befriedigender Bekämpfungserfolg gegenüber dem Getreideplattkäfer wurde bei 20°C (LC50 921–1335 IL/Adul-tem) erreicht. Die Konzentration der verwendeten Nemato-densuspension beeinflusste den Bekämpfungserfolg gegenüber beiden Schädlingsarten dagegen weniger. Obwohl die Verwendung entomopathogener Nematoden zur Kontrolle der beiden untersuchten Vorratsschädlinge des Getreides zur Zeit noch nicht praktikabel ist, könte dieser Ansatz zukünftig in Kombination mit anderen (biotechnischen) Verfahren zum Erfolg führen.

Stichwörter

biologische Bekämpfung Effizienz entomo-pathogen Nematoden Oryzaephilus surinamensis Sitophilus granarius 

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Copyright information

© Deutsche Phythomedizinische Gesellschaft 2006

Authors and Affiliations

  1. 1.Biotechnical Faculty, Dept. of Agronomy, Chair of Entomology and PhytopathologyUniversity of LjubljanaLjubljanaSlovenia
  2. 2.University of Ljubljana, Biotechnical Faculty, Dept. of Agronomy, Chair of Fodder Production and Pasture ManagementLjubljanaSlovenia

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