Abstract
Phenolic compounds of three scab-resistant apple cultivars ‘Topaz’, ‘Goldstar’ and ‘Gold Rush’ and two susceptible ones ‘Golden Delicious Weinsberg’ and ‘Golden Delicious Clone B’ were studied. The leaves were sampled four times in the period from 12 June to 10 September 2001. Flavonols rutin, quercitrin and ‘phenol 173’ (phenol with retention time 173,38 min) were analyzed using the Hplc system. Between the resistant and susceptible cultivars, statistically significant differences were established in the relative content of ‘phenol 173’, which was higher in the resistant cultivars during the growing season. It appears that the content is not affected either by different environmental conditions or by different stressors, but only by the genotype. Differences in the content of rutin and quercitrin were not significant. During the growth period, the content of phenolic compounds changed and was related to the physiological stage of the tissue and cultivar used.
Zusammenfassung
Die phenolischen Verbindungen in den drei gegen Schorf resistenten Apfelsorten ‘Topaz’, ‘Goldstar’ und ‘Gold Rush’ und in den zwei für Schorf anfälligen Sorten ‘Golden Delicious Weinsberg’ und ‘Golden Delicious Clone B’ wurden untersucht. Die Blätter für die Analyse wurden an vier verschiedenen Terminen zwischen dem 12. Juni und 10. September 2001 gesammelt. Die Flavonole Rutin und Quercitrin und ‘Phenole 173’ (Phenole mit der Retentionszeit 173,38 min) wurden mit Hilfe des Hplc bestimmt. Bei ‘Phenole 173’ konnten statistische Unterschiede gefunden werden. Die Gehalte dieser Substanz waren bei den resistenten Sorten während der ganzen Messperiode höher als bei den Standards. Sie wurden weder von verschiedenen Klimaverhältnissen noch von unterschiedlichen Stressfaktoren beeinflusst, sondern nur vom Genotyp. Die Gehaltsunterschiede bei den anderen Flavonolen ließen sich nicht absichern. Die Gehalte der phenol ischen Verbindungen veränderten sich in der Wachstumsperiode und waren vom physiologischen Status des Gewebes und von der Sorte abhängig.
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Literature
Agrios, N. G.: Plant pathology. 4th edition? — Academic Press San Diego, 1997.
Barnes, E. H., E. B. Williams: A biochemical response of apple tissues to fungus infection. — Phytopathology 50, 844–846, 1960.
Bennett, R. N., R. M. Wallsgrove: Secondary metabolites in plant defence mechanisms. — New Phytologist 127, 617–633, 1994.
Mayr, U., R. Vatzdorfer, D. Treutter, W. Feucht: Surfactant-induced changes in phenol content of apple leaves and fruit skins. — Acta Horticulturae 381, 479–87, 1994.
Mayr, U., S. Michalek, D. Treutter, W. Feucht: Phenolic compounds of apple and their relationship to scab resistance. — J. Phytopathol. 145, 69–75, 1997.
Mayr, U., D. Treutter, C. Santos-Buelga, H. Vauer, W. Feucht: Developmental changes in the phenol concentrations of ‘Golden delicious’ apple fruits and leaves. — Phytochemistry 38, 1151–1155, 1995.
Michalek, S., U. Mayr, D. Treutter, A. Lux-Endrich, M. Gutmann, W. Feucht, M. Geibel: Role of flavan-3-ols in resistance of apple trees to Venturia inaequalis. — Acta Horticulturae 484, 535–539, 1999.
Michalek, S., D. Treutter, U. Mayr, A. Lux-Endrich, A. Gutmann, W. Feucht: Role of flavan-3-ols in resistance of apple trees to Venturia inaequalis. In: Vercauteren, J., C. Cheze, M. Dumon, J. Weber (eds.): Polyphenols communications 96, pp. 26–27, 1996.
Niederleitner, S., V. Zinkernagel, D. Treutter, W. Feucht: Accumulation of flavanols in cherry leaves after infection by the fungus Blumeriella jaapii. — Acta Horticulturae 381, 767–770, 1994.
Picinelli, A., E. Dapena, J. J. Mangas: Polyphenolic pattern in apple tree leaves in relation to scab resistance. A preliminary study. — J. Agricult. Food Chem. 43, 2273–2278, 1995.
Rat-Morrise, E., M. J. Amiot, M. Tacchini, Y. Lespinass: Phenolic composition of apple leaves, prior to infestation, associated with the resistance of the apple cv. Florina-Querina to the apple scab Venturia inaequalis and to the rosy apple aphid Dysaphis plantaginea. — In: Vercauteren J., C. Cheze, M. Dumon, J. Weber (eds.): Polyphenols Communications 96, pp. 355–356, 1996.
Rühl, K., W. Hatmann, R. Stösser: Gehalt phenolischer Verbindungen in gesunden und scarka-kranken Blättern von Prunus domestica. — Z. PflKrankh. PflSchutz 99, 569–577, 1992.
Treutter, O.: Separation of naturally occurring mixtures of phenolic compounds from various Prunus tissues by reversed-phase high-performance liqud chromatography. — J. Chromatography 436, 490–496, 1988.
Treutter, D., W. Feucht: The pattern of flavan-3-ols in relation to scab resistance of apple cultivars. — J. Horticult. Sci. 65, 511–517, 1990.
Treutter, D., C. Santos-Buelga, M. Gutmann, H. Kolodziej: Identification of flavan-3-ols and procyanidins by high-performance chromatography and chemical reaction detection. — J. Chromatography 667, 290–297, 1994.
Usenik, V., F. Štampar: Influence of various rootstocks for cherries on p-coumaric acid, genistein and prunin content and their involvement in the incompatibility process. — Gartenbauwissenschaft 65, 245–250, 2000.
Usenik, V., F. Štampar: Influence of scion/rootstock interaction on seasonal changes of phenols. — Phyton (Horn) 42, 279–289, 2002.
Viršček Marn, M., F. Štampar, B. Javornik: Screening for scab resistance by Rapd markers in cultivars of apple (Malus spp). — Pl. Breeding 115, 488–493, 1996.
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Usenik, V., Mikuli-Petkovšek, M., Solar, A. et al. Flavonols of leaves in relation to apple scab resistance. J Plant Dis Prot 111, 137–144 (2004). https://doi.org/10.1007/BF03356139
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DOI: https://doi.org/10.1007/BF03356139