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Journal of Plant Diseases and Protection

, Volume 110, Issue 5, pp 449–460 | Cite as

Development of UV-induced Carbendazim-resistant mutants of Trichoderma harzianum for integrated control of damping-off disease of cotton caused by Rhizoctonia solani

  • J. Jayaraj
  • N. V. Radhakrishnan
Article

Abstract

The treatment of seeds with Trichoderma harzianum has been used successfully to control damping-off of cotton and other crops. However, T. harzianum is very sensitive to carbendazim which is often used as a seed-dressing fungicide for the control of internally seed-borne fungal pathogens. Hence in the present study, carbendazim-resistant mutants of T. harzianum were developed through UV-irradiation. These mutants grew well in PDA medium containing the carbendazim even at 100 μg/ml. Sustained production of cellulase and chitinase by these mutants in the presence of carbendazim in the growth medium was observed. These mutants established well in the rhizosphere of cotton plants. Seed treatment with carbendazim followed by application of carbendazim-resistant mutants of T. harzianum resulted in better plant stand, plant biomass and less damping-off disease caused by R. solani in both greenhouse and field conditions.

Key words

Carbendazim cotton damping-off integrated control Rhizoctonia solani Trichoderma harzianum mutants 

Entwicklung von UV-induzierten Carbendazim-resistenten Mutanten von Trichoderma harzianum zur integrierten Bekämpfung durch die durch Rhizoctonia solani verursachte Umfallkrankheit an Baumwolle

Zusammenfassung

Die Behandlung von Saatgut mit Trichoderma harzianum hat bei der Bekämpfung der Umfallkrankheit an Baumwolle und anderen Kulturpflanzenarten zu guten Erfolgen geführt. T. harzianum ist jedoch sehr empfindlich gegenüber dem Fungizid Carbendazim, welches häufig zur Beizung von Saatgut zur Bekämpfung von samenbürtigen pilzlichen Krankheitserregern eingesetzt wird. In der vorliegenden Arbeit wird die Erzeugung von Carbendazim-resistenten Mutanten von T. harzianum durch UV-Bestrahlung beschrieben. Diese Mutanten wuchsen gut auf PDA, dem 100 μg/ml Carbendazim zugesetzt waren. In diesem Medium wurde auch eine nachhaltige Bildung von Zellulase und Chitinase beobachtet. Diese Mutanten etablierten sich gut in der Rhizosphäre von Baumwollpflanzen. Saatgutbehandlung mit Carbendazim und nachfolgende Applikation von Carbendazim-resistenten Mutanten von T. harzianum führten unter Gewächshaus- und Freilandbedingungen zu einem besseren Pflanzenbestand, zu einer größeren Biomasse sowie zu einem verminderten Befall mit der durch R. solani verursachten Umfallkrankheit.

Stichwörter

Carbendazim Baumwolle Umfallkrankheit integrierte Bekämpfung Rhizoctonia solani Trichoderma harzianum Mutanten 

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Copyright information

© Deutsche Phythomedizinische Gesellschaft 2003

Authors and Affiliations

  1. 1.Department of Plant PathologyAnnamalai UniversityAnnamalai nagarIndia
  2. 2.Department of Plant Pathology, Regional Agricultural Research StationKerala Agricultural UniversityAmbalavayalIndia

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