Abstract
Fusarium semitectum, which occurs frequently among the fungal microflora associated with seedling disease of cotton, was examined using traditional mycological characteristics and molecular methods. The 29 Fusarium semitectum isolates from different cotton-producing areas and different host genotypes in Egypt could be divided undoubtedly into five groups based on cultural characteristics or Dna fingerprinting methods. The isolates showed a high level of variability in aerial mycelia growth, mycelia colour and radial mycelia growth (colony diameter) on potato dextrose agar. The intra-specific genomic relatedness of F. semitectum isolates was detected by Aflp and Rapd-Pcr fingerprinting methods and no particular difference was found between the results with the two fingerprint techniques.
Five genomic groups were obtained with both Rapd-Pcr and Aflp techniques. A good correlation of the five genomics groups and cultural characteristics were found, but no correlation between the results of the genetic analysis with the host genotype and geographic origin could be observed. The results indicate the utility of Rapd-Pcr and Aflp for identifying and monitoring intra-specific genetic variability for F. semitectum isolates.
Zusammenfassung
Fusarium semitectum, ein Pathogen, das häufig mit anderen Sämlingskrankheiten assoziiert ist, wurde mit traditionellen mykologischen und molekularen Techniken untersucht. Die 29 Isolate, die aus unterschiedlichen Anbauregionen der Baumwolle und Wirtsgenotypen isoliert wurden, konnten aufgrund ihrer morphologischen Charakteristika und der Ergebnisse aus den Fingerprints eindeutig in fünf Gruppen aufgeteilt werden. Die Isolate zeigten eine hohe Variabilität im Luftmycelwachstum, in der Pigmentierung und im radialem Myzelwachstum auf Pd-Agar.
Die intraspezifische, genomische Verwandtschaft der Isolate konnte mit der Aflp- und Rapd-Pcr Fingerprint-Methode nachgewiesen werden, wobei keine wesentlichen Unterschiede in den Ergebnissen der beiden Fingerprint-Techniken entdeckt wurden. Es konnten ebenfalls fünf genomische Gruppen abgeleitet werden. Allerdings konnte keine Korrelation zwischen den Ergebnissen der Fingerprint-Analyse und dem Wirtsgenotyp bzw. der Herkunft der Isolate hergestellt werden.
Die Ergebnisse zeigen, dass mit der Rapd-Pcr und Aflp-Technik eine Klassifizierung von F. semitectum möglich ist und die intraspezifische, genetische Variabilität bestimmt werden kann.
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Abd-Elsalam, K.A., Schnieder, F., Asran-Amal, A. et al. Intra-species genomic groups in Fusarium semitectum and their correlation with origin and cultural characteristics. J Plant Dis Prot 110, 409–418 (2003). https://doi.org/10.1007/BF03356118
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DOI: https://doi.org/10.1007/BF03356118