Journal of Endocrinological Investigation

, Volume 7, Issue 4, pp 323–326 | Cite as

Granulosa and thecal cells from human ovarian follicles under growth: steroid formation in vitro and responsiveness to human chorionic gonadotropin

  • L. Nilsson
  • B. Dennefors
  • L. Hamberger


In order to characterize the patterns of steroid production and gonadotropin responsiveness in growing human follicles, follicular thecal and granulosa cells were incubated for two hours in the presence or absence of human chorionic gonadotropin (hCG). After incubation, tissue cyclic AMP (cAMP) levels and medium content of progesterone (P), androstenedione (A) and estradiol-17β (E) were determined. A was the dominant steroid formed by the thecal cells, regardless if these were derived from small (diameter: 4–7.5 mm) or from large (diameter: 8–15 mm) follicles. Granulosa cells from small follicles formed minimal amounts of all steroids measured, while granulosa cells from large follicles produced considerable amounts of E in vitro. Thecal cells from both small and large follicles increased their production of cAMP in the presence of hCG. Steroid formation was significantly increased by hCG in thecal cells from large follicles only. Granulosa cells from large follicles responded to hCG in vitro with increased cAMP and steroid formation, while granulosa cells from small follicles appeared insensitive to hCG in vitro.


Human follicle granulosa theca steroids cyclic AMP gonadotropin 


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  1. 1.
    McNatty K.P. Hormonal correlates of follicular development in the human ovary. Aust. J. Biol. Sci. 34: 249, 1981.PubMedGoogle Scholar
  2. 2.
    Richards J. Maturation of ovarian follicles: actions and interactions of pituitary and ovarian hormones on follicular cell differentiation. Physiol. Rev. 60: 51, 1980.PubMedGoogle Scholar
  3. 3.
    Dennefors B.L., Nilsson L., Hamberger L. Steroid and adenosine 3’5’-monophosphate formation in granulosa and thecal cells from human preovulatory follicles in response to human chorionic gonadotrophin. J. Clin. Endocrinol. Metab. 54: 436, 1982.PubMedCrossRefGoogle Scholar
  4. 4.
    Lowry O.H., Rosebrough N.J., Farr A.L., Randall R.J. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193: 265, 1951.PubMedGoogle Scholar
  5. 5.
    Gilman A.G. A protein binding assay for adenosine 3’5’-cyclic monophosphate. Proc. Natl. Acad. Sci. USA 67: 305, 1970.PubMedCentralPubMedCrossRefGoogle Scholar
  6. 6.
    McNatty K.P., Makris A., Osathanondh R., Ryan K.J. The microenvironment of the human antral follicle: interrelationships among the steroid levels in antral fluid, the population of granulosa cells, and the status of the oocyte in vivo and in vitro. J. Clin. Endocrinol. Metab. 49: 851, 1979.PubMedCrossRefGoogle Scholar
  7. 7.
    Rajaniemi H.J., Vanha-Perttula T. Specific receptor for LH in the ovary: evidence by autoradiography and tissue fractionation. Endocrinology 90: 1, 1972.PubMedCrossRefGoogle Scholar
  8. 8.
    Tsang B.K., Moon Y.S., Simpson C.W., Amstrong D.T. Androgen biosynthesis in human ovarian follicles: cellular source, gonadotropic control, and adenosine 3’5’-monophosphate mediation. J. Clin. Endocrinol. Metab. 48: 153, 1979.PubMedCrossRefGoogle Scholar
  9. 9.
    McNatty K.P., Makris A., Osathandondh R., Ryan K.J. Effects of luteinizing hormone on steroidogenesis by thecal tissue from human ovarian follicles in vitro. Steroids 36: 53, 1980.Google Scholar
  10. 10.
    Moon Y.S., Tsang B.K., Simpson C, Armstrong D.T. 17β-estradiol biosynthesis in cultured granulosa and thecal cells of human ovarian follicles: stimulation by follicle stimulating hormone. J. Clin. Endocrinol. Metab. 47: 263, 1978.PubMedCrossRefGoogle Scholar
  11. 11.
    Hillier S.G., van den Boogard A.M.J., Reichert Jr. L.E., van Hall E. Intraovarian sex steroid hormone interactions and the regulation of follicular maturation: aromatization of androgens by human granulosa cells in vitro. J. Clin. Endocrinol. Metab. 50: 640, 1980.PubMedCrossRefGoogle Scholar
  12. 12.
    Erickson G.F., Hsueh A.J.W., Quigley M.E., Rebar M.W., Yen S.S.C. Functional studies of aromatase activity in human granulosa cells from normal and polycystic ovaries. J. Clin. Endocrinol. Metab. 49: 514, 1979.PubMedCrossRefGoogle Scholar
  13. 13.
    Rajaniemi HJ., Rönnberg L, Kaupplila A., Ylöstalo P., Jalkanen M., Saastamoinen J., Seiander K., Pystynen P., Vihko R. Luteinizing hormone receptors in human ovarian follicles and corpora lutea during menstrual cycle and pregnancy. J. Clin. Endocrinol. Metab. 52: 307, 1981.PubMedCrossRefGoogle Scholar
  14. 14.
    McNatty K.P., Makris A., De Grazia C., Osathanondh R., Ryan K.J. The production of progesterone, androgens and estrogens by granulosa cells, thecal tissue, and stromal tissue from human ovaries in vitro. J. Clin. Endocrinol. Metab. 49: 687, 1979.PubMedCrossRefGoogle Scholar
  15. 15.
    Moon Y.S., Duleba A., Leung P.C.S., Gomel V. Metabolism of testosterone by human granulosa cells in culture: influence of follicle-stimulating hormone and luteinizing hormone. Am. J. Obstet. Gynecol. 142: 692, 1982.PubMedGoogle Scholar
  16. 16.
    Richards J.S., Midgley A.R. Protein hormone action: a key to understanding ovarian follicular and luteal cell development. Biol. Reprod. 14: 82, 1976.PubMedCrossRefGoogle Scholar

Copyright information

© Italian Society of Endocrinology (SIE) 1984

Authors and Affiliations

  • L. Nilsson
    • 1
  • B. Dennefors
    • 1
  • L. Hamberger
    • 1
  1. 1.Department of Obstetrics and GynecologyUniversity of GöteborgGöteborgSweden

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