Abstract
This paper describes the identification of circulating autoantibodies to thyroid hormones in 5 patients with Graves’ disease (n = 2), Hashimoto’s thyroiditis (n = 1), primary myxedema (n = 1) and Sjögren syndrome (n = 1) out of 351 individuals. The 351 patients suffered from different autoimmune thyroid disorders, thyroid cancer or lymphoreticular system disorders as well as autoimmune non thyroid illnesses. Immunoglobulin G (IgG) binding of one or both tracer thyroid hormones and in one case also of reverse triiodothyronine, was demonstrated by radioimmunoprecipitation experiments and by reverse flow zone electrophoresis or cellulose acetate electrophoresis and autoradiography. In all cases the binding of radiolabeled thyroid hormones to IgG was displaced by an excess of the respective non radioactive thyroid hormone. Thyroglobulin displaced the binding in 2 cases but preadsorption with human thyroglobulin was ineffective in other 2 cases. Radioimmunoelectrophoresis and autoradiography of the Immunoelectrophoresis plates indicated that in 2 cases the light chains involved in the binding were k chains of the G3 subclass and to a lesser extent of the G1 subclass, in another case they were λ chains and therefore of restricted heterogeneity. In a case of myxedema coma IgG were polyclonal (k and λ chains of the G1 > G2 > G3 subclasses). The thyroxine (T4) or triiodothyronine (T3) antibodies interfere with the hormone measurement in unextracted serum by falsely lowering their concentrations when non specific separation methods are used and raising their concentrations when double antibody radioimmunoassays are used.In one serum containing anti-T4 antibodies, the association constant (Ka) was 0.63 × 106 l/M and absolute binding (Ab0) was1.54 x10-7 l/M. In another serum with anti-T3 IgG, Ka was 4.44 × 107 l/M and Abo 6.22 × 10-9 l/M. In the case of myxedema coma Ka ranged between 3.1 and 4.3 × 108 l/M.
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References
Robbins J., Rall J.E. An unusual instance of thyroxine binding by human serum gammaglobulin. J. Clin. Endocrinol. Metab. 16: 573, 1956.
Premachandra B.N., Blumenthal H.T. Abnormal binding of thyroid hormones in sera from patients with Hashimoto’s disease. J. Clin. Endocrinol. Metab. 27: 931, 1967.
Ochi Y., Shiomi K., Hachiva T., Yoshimura M., Miyazaky T. Immunological analysis of abnormal binding of thyroid hormone in the gamma-globulin. J. Clin. Endocrinol. Metab. 35: 743, 1972.
Sterling K., Milch P.O., Holub D.A., Berger A. Evidence that triiodothyronine (T3) is not the only active form of thyroid hormone. Program of the 49th Annual Meeting of the American Thyroid Association, 1973, p. T115 (Abstract).
Schussler G.C., Howard S.F. T3 determination and T4 to T3 conversion in T3 immune rabbits: a model for the man without T3. Program of the 56th Annual Meeting of the’Endocrine Society, 1974, p. A92 (Abstract).
Staeheli V., Valloton M.B., Burger A. Detection of human antithyroxine and antitriiodothyronine antibodies in different thyroid conditions. J. Clin. Endocrinol. Metab. 41: 669, 1975.
Wu S.Y., Green W.L. Triiodothyronine (T3)- binding immunoglobulin in a euthyroid woman: effects on T3(RIA) and on T3 turnover. J. Clin. Endocrinol. Metab. 42: 642, 1976.
Herrmann J., Rudorff K.H., Kroner H., Premachandra B.N. Antibody binding of thyroid hormone in juvenile goitrous hypothyroidism. Horm. Metab. Res. 9: 394, 1977.
Hehrman R., Höffken B., von zur Mühlen A., Creutzig H., Thiele J., Hesch R.D. Antithyroid hormone autoantibodies under experimental and clinical conditions. Horm. Metab. Res. 9: 326, 1977.
Karlsson F.A., Wibell L., Wide L. Hypothyroidism due to thyroid hormone binding autoantibodies. N.Engl. J. Med. 296:1146, 1977.
Blanc M., Despont A., Burger A. Thyroid hormone binding antibodies. N.Engl. J. Med. 296:1068, 1977.
Ginsberg J., Segal D., Erlich R.M., Walfish P.G. Inappropriate triiodothyronine (T3) and thyroxine (T4) radioimmunoassay levels secondary to circulating thyroid hormone autoantibodies. Clin. Endocrinol. (Oxf.) 8:133, 1978.
Walfish P.G., Ginsberg J., Premachandra B.N. Circulating autoantibodies to reverse T3 (rT3), thyroxine (T4) and triiodothyronine (T3): effects of exogenous thyroid hormones. Ann. Endocrinol. (Paris) 40: 30A, 1979 (Abstract).
Ikekubo K., Junijj K., Endo K., Nakajima K., Okuno T., Kasagi K., Mori T., Nagata I., Torizuka K. Antithyroxine and antitriiodothyronine antibodies in three cases of Hashimoto’s thyroiditis. Acta Endocrinol. (Kbh.) 89: 557, 1978.
Clayton-Hopkins J.A., Schussler G.C., Rule A.M., DeLellis R.A., Lassman M.N. Binding of triiodothyronine (T3) to circulating autoantibodies in a euthyroid woman. Program of the 60th Annual Meeting of the Endocrine Society, Miami FL, USA, 1978, p. 118 (Abstract).
Krüskemper H.L., Herrmann J. Free thyroid hormones and thyroid hormone binding autoantibodies. In: Ekins R., Faglia G., Pennisi F., Pinchera A. (Eds.) Free thyroid hormones. Excerpta Medica, Amsterdam, 1979, p. 240.
Jorgensen P.H., Skovsted L., Jensen K.A. Abnormal gamma globulin binding of thyroid hormones. Acta Med. Scand. 205: 375, 1979.
Faerch T., Hojslet P.E., Mose C., Paby P. Thyroid hormone autoantibodies in a case of Hashimoto’s disease. Scand. J. Clin. Lab. Invest. 40: 203, 1980.
Pearce C.J., Byfield P.G.H., Edmonds C.J., Lalloz M.R A., Himsworth R.L. Autoantibodies to thyroglobulin crossreacting with iodo-thyronines. Clin. Endocrinol. (Oxf.) 15: 1, 1981.
Teuber J., Helmke K., Mäser E., Grebe S.F., Federlin K. Evidence of endogenic antibodies (Ab) against thyroid hormones and their clinical relevance. Acta Endocrinol. (Kbh.) 96 (suppl. 240): 25, 1981.
Geola F.L., Hershman J.M., Reed A.W., Premachandra B.N. Circulating thyroid hormone autoantibodies in a hypothyroid patient: effect on thyroxine metabolic clearance rate. J. Clin. Endocrinol. Metab. 53: 580, 1981.
Beck Peccoz P., Romelli P.B., Cattaneo M.G., Faglia G. Antiiodothyronine antibodies: incidence in thyroid disorders and interference in total thyroid hormone radioimmunoassay. Ann. Endocrinol. (Paris) 42: 46A, 1981 (Abstract).
Merlin P., Mongardi L., Rapetti C., De Filippis V. Autoanticorpi anti-T4,-T3,-rT3 in due pazienti tireopatiche. 2nd Giornate Toscane delta Tiroide Pisa, March 12–13 1982, p. 61 (Abstract).
Trimarchi F., Benvenga S., Fenzi G.F., Mariotti S., Consolo F. Immunoglobulin binding of thyroid hormones in a case of Waldenstrom’s macroglobulinemia. J. Clin. Endocrinol. Metab. 54:1045, 1982.
Filetti S., Galbiati A., Trimarchi F., Vigneri A radioimmunoassay for thyroxine in unextracted serum. J. Nucl. Biol. Med. 79:86, 1975.
Romelli P.B., Pennisi F., Vancheri L. Measurements of free thyroid hormones in serum by column adsorption chromatography and radioimmunoassay. J. Endocrinol. Invest. 2: 25, 1979.
Mori T., KrissJ. P. Measurement by competitive binding radioassay of serum antimicrosomal and antithyroglobulin antibodies in Graves’ disease and other thyroid disorders. J. Clin. Endocrinol. Metab. 33: 688, 1971.
Pinkard R.N., Weir D.M. Equilibrium dialysis and preparation of hapten conjugates. In: Weir D.M. (Ed.) Handbook of experimental immunology, ed. 2. Blackwell, Oxford, 1978, p. 61.
Walfish P.G., Premachandra B.N. Masking of circulating thyroxine autoantibodies in chronic thyroiditis patients on thyroxine therapy: detection by charchoal stripping. Clin. Res. 280: 270A, 1980 (Abstract).
Premachandra B.N., Walfish P.G. Effects and clinical significance of exogenous thyroxine therapy in patients with circulating thyroid hormones autoantibodies. Am. J. Clin. Pathol. 78:63, 1982.
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Trimarchi, F., Benvenga, S., Costante, G. et al. Identification and characterization of circulating thyroid hormone autoantibodies in thyroid diseases, in autoimmune non thyroid illnesses and in lymphoreticular system disorders. J Endocrinol Invest 6, 203–209 (1983). https://doi.org/10.1007/BF03350609
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DOI: https://doi.org/10.1007/BF03350609