Advertisement

Journal of Endocrinological Investigation

, Volume 5, Issue 5, pp 287–292 | Cite as

Binding and degradation of 125l-insulin in human erythrocytes. Comparative studies with hemolysate and membranes.

  • G. Bellomo
  • R. Bellazzi
  • P. L. Nicotera
  • R. Francesconi
  • P. Fratino
Article

Abstract

I nsulin degradation in human erythrocytes obtained from normal subjects is studied. Under the experimental conditions used, insulin binding and insulin degradation are strictly correlated, and no degrading activity is released in incubation medium. Comparative analysis of hemolysate and membranes shows that hemolysate possesses a definite insulin-degrading activity which is not linked to the hemoglobin molecule; the km of this enzymatic activity is of the same order of magnitude as that reported in other cells. At the concentration and in the condition used in this study, membranes degrade insulin at a lower rate than hemolysate.

Key-words

Insulin receptor insulin binding insulin degradation human erythro cyte 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Roth J. Receptors for insulin, NSILA-s and growth hormone. Applications to disease states in man. Recent Prog. Horm. Res. 31: 95, 1975.PubMedGoogle Scholar
  2. 2.
    Gorden P., Carpentier J.R., Freychet P., LeCam A., Orci L. Intracellular translocation of 125l-labelled insulin: direct demonstration in isolated hepatocytes. Science 200: 782, 1978.PubMedCrossRefGoogle Scholar
  3. 3.
    Carpentier J.L., Gorden P., Freychet P., LeCam A., Orci L. Lysosomial association of internalized 125l-insulin in isolated rat hepatocytes. J. Clin. Invest. 63: 1249, 1979.PubMedCentralPubMedCrossRefGoogle Scholar
  4. 4.
    Suzuky K., Kono T. Internalization and degradation of fat cell bound insulin. J. Biol. Chem. 254: 9786, 1979.Google Scholar
  5. 5.
    Freychet P., Kahn C.R., Roth J., Neville D.M. Jr. Insulin interaction with liver plasma membranes. Independence of binding of the hormone and its degradation. J. Biol. Chem. 247: 3953, 1972.PubMedGoogle Scholar
  6. 6.
    Kahn C.R., Freychet P., Neville D.M., Jr. Roth J. Quantitative aspects of the insulin-receptor interaction in liver plasma membranes. J. Biol. Chem. 249: 2249, 1974.PubMedGoogle Scholar
  7. 7.
    Hammon J.M., Jarrett L. Insulin degradation by isolated fat cells and their subcellular fractions. Diabetes 24: 1011, 1975.CrossRefGoogle Scholar
  8. 8.
    Lang U., Kahn C.R., Chrambach A. Characterization of the insulin receptor and insulin-degrading activity from human lymphocytes by quantitative Polyacrylamide gel electrophoresis. Endocrinology 106: 40, 1980.PubMedCrossRefGoogle Scholar
  9. 9.
    Gliemann J., Sonne O. Binding and receptor-mediated degradation of insulin in adipocytes. J. Biol. Chem. 253: 7857, 1978.PubMedGoogle Scholar
  10. 10.
    Gammeltoft S., Gliemann J. Binding and degradation of 125l-labelled insulin by isolated rat fat cells. Biochim. Biophys. Acta 320: 16, 1973.PubMedCrossRefGoogle Scholar
  11. 11.
    Terris S., Steiner D.F. Binding and degradation of 125l-insulin by rat hepatocytes. J.Biol. Chem. 250: 8389, 1975.PubMedGoogle Scholar
  12. 12.
    Baldwin D., Terris S., Steiner D.F. Insulin receptor antibody inhibits insulin degradation. Diabetes 28: (Suppl. 1): 392, 1979.Google Scholar
  13. 13.
    Marshall S., Olefsky J.M. Effect of lisosomotropic agents on insulin interaction with adipocytes. J. Biol. Chem. 254: 10153, 1979.PubMedGoogle Scholar
  14. 14.
    Gambhir K.K., Archer J.A., Bradley C.J. Characteristics of human erythrocyte insulin receptors. Diabetes 27: 701, 1978.PubMedCrossRefGoogle Scholar
  15. 15.
    Boyoum A. Separation of lymphocytes from blood and bone marrow. Scand. J. Clin. Lab. Invest. 21 (Suppl. 97): 77, 1968.Google Scholar
  16. 16.
    Hennessey M.A., Waltersdorph A.M., Huennekens F.M., Gabrio B.W. Erythrocyte metabolism. VI. Separation of erythrocyte enzymes from hemoglobin. J.Clin. Invest. 41: 1257, 1962.PubMedCentralPubMedCrossRefGoogle Scholar
  17. 17.
    Lowry H.O., Rosebrough N.J., Farr A.L., Randall R.J. Protein measurement with the Folin phenol reagent. J.Biol. Chem. 53: 265, 1951.Google Scholar
  18. 18.
    Misbin R.I., Nehl T.D. Insulin receptor binding and degradation in IM-9 cultured human lymphocytes. Importance of extracellular degradation. J. Endocrinol. Invest. 3: 77, 1980.PubMedCrossRefGoogle Scholar
  19. 19.
    Misbin R.I., Davis J.G., Offord R.E., Halban P.A., Nehl T.D. Binding and degradation of semisynthetic tritiated insulin by IM-9 cultured human lymphocytes. Diabetes 29: 730, 1980.PubMedCrossRefGoogle Scholar
  20. 20.
    Sonne O., Gliemann J. Insulin receptors of cultured human lymphocytes (IM-9). J. Biol. Chem. 255: 7449, 1980.PubMedGoogle Scholar
  21. 21.
    Olefsky J.M., Johnson J., Liu F., Edwards P., Baur S. Comparison of 125l-insulin binding and degradation in isolated rat hepatocytes and liver membranes. Diabetes 24: 801, 1978.CrossRefGoogle Scholar
  22. 22.
    LeCam A., Freychet P., Lenoir P. Degradation of insulin by isolated rat liver cells. Diabetes 24: 866, 1978.Google Scholar
  23. 23.
    Duckworth W.C. Insulin and glucagone degradation by isolated kidney cell membranes. Endocrinology 102: 1766, 1978.PubMedCrossRefGoogle Scholar
  24. 24.
    Yokono K., Imamura Y., Sakay H., Baba S. Insulin degrading activity of plasma membranes from rat skeletal muscle. Diabetes 28: 810, 1979.PubMedCrossRefGoogle Scholar
  25. 25.
    Gambhir K.K., Nerurkar S.G., Das P.D., Archer J.A., Henry L. Insulin binding and degradation by human erythrocytes at physiological temperature. Endocrinology 109: 1797, 1981.CrossRefGoogle Scholar

Copyright information

© Italian Society of Endocrinology (SIE) 1982

Authors and Affiliations

  • G. Bellomo
    • 1
  • R. Bellazzi
    • 1
  • P. L. Nicotera
    • 1
  • R. Francesconi
    • 1
  • P. Fratino
    • 1
  1. 1.Clinica Medica IIUniversità di Pavia, Policlinico San MatteoPaviaItaly

Personalised recommendations