Journal of Endocrinological Investigation

, Volume 1, Issue 3, pp 197–201 | Cite as

Kinetics of sodium in rabbit arterial wall: inability of aldosterone to alter extra to intracellular distribution

  • J. G. Llaurado
  • G. A. Smith


Transport rate constants (kij) describing the kinetics of Na exchanges in isolated rabbit aorta wall were determined by a previously established method involving the use of 22Na as a tracer and digital computer simulation without recourse to ancillary chemical measurements of extracellular space. A three compartment model consisting of (i) extracellular, (ii) intracellular and (iii) subcellular spaces (compartments) was found to describe adequately the kinetics of 22Na. Normative values for intercompartmental kij and extra to intracellular Na ratio were established. It appears that the Na extracellular space in rabbit arterial wall is larger than that in dog or rat arterial wall. Surprisingly, at variance with several tissues of different species (dog, rat, mouse and human tissues), aldosterone did not influence the extra to intracellular distribution of Na. The findings are interpreted in the light of results obtained previously by other workers using entirely unrelated methodologies and suggest that species difference is an important factor to consider when studying effects of aldosterone on tissue electrolyte distribution in the rabbit.


Aldosterone arterial wall compartmental analysis computer simulation continuous outflow extracellular-intracellular rabbit radionuclide tracers SAAM computer program sodium kinetics 


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  1. 1.
    Llaurado J.G. Compartimentai approaches to water and electrolyte distribution. In: Brown J.H.U., Gann D.S. (Eds), Engineering Principles in Physiology. Academic Press, New York, 1973, vol. 2, p. 347.Google Scholar
  2. 2.
    Török J., Nedergaard O.A., Bevan J.A. Distribution of inulin space in the rabbit thoracic aorta. Experientia 27: 55, 1971.PubMedCrossRefGoogle Scholar
  3. 3.
    Harrison R.G., Massaro T.A. Extracellular space of swine aorta measured with [14C] inulin and [14C] sucrose. Amer. J. Physiol. 231: 1806, 1976.PubMedGoogle Scholar
  4. 4.
    Llaurado J.G. Digital computer simulation as an aid to the study of arterial wall Na kinetics. J. Appl. Physiol. 27: 544, 1969.PubMedGoogle Scholar
  5. 5.
    Llaurado J.G. Some effects of aldosterone on sodium transport rate constants in isolated arterial wall: studies with computer simulation and analysis. Endocrinology 87: 517, 1970.PubMedCrossRefGoogle Scholar
  6. 6.
    Smith G.A., Llaurado J.G. Computer modeling of nonsteady state sodium kinetics in liver. IEEE Trans. Biomed. Eng. 21: 433, 1974.Google Scholar
  7. 7.
    Llaurado J.G., Madden J.A. Sodium kinetics in aorta of spontaneously hypertensive rats. J. Appl. Physiol. 39: 868, 1975.PubMedGoogle Scholar
  8. 8.
    Genest J., Lemieux G., Davignon A., Loiw E., Nowaczynski W., Steyermark P. Human arterial hypertension: a state of mild chronic hyperaldosteronism? Science 123: 503, 1956.PubMedCrossRefGoogle Scholar
  9. 9.
    Conn J.W., Cohen E.L., Rovner D.R., Nesbit R.M. Normokalemic primary aldosteronism: a detectable cause of curable “essential hypertension”. J.A.M.A. 193: 200, 1965.PubMedCrossRefGoogle Scholar
  10. 10.
    Conn J.W., Rovner D.R., Cohen E.L, Nesbit R.M. Normokalemic primary aldosteronism: its masquerade as ‘essential hypertension’ J.A.M.A. 195: 21, 1966.CrossRefGoogle Scholar
  11. 11.
    Kumar D., Hall A.E.D., Nakashima R., Gornall A.G. Studies on aldosterone: II. Hypertension as a cumulative effect of aldosterone administration. Can. J. Biochem. Physiol. 35: 113, 1957.PubMedCrossRefGoogle Scholar
  12. 12.
    Masson G.M.C., Mikasa A., Yasuda H. Experimental vascular disease elicited by aldosterone and renin. Endocrinology 71: 505, 1962.PubMedCrossRefGoogle Scholar
  13. 13.
    Hollander W., Kramsch D.M., Chobanian A.V., Melby J.C. Metabolism and distribution of intravenously administered d-aldosterone-1,2-H3 in the arteries, kidneys, and heart of dog. Circ. Res. 18–19: 1–35, 1966.Google Scholar
  14. 14.
    Bevan J.A., Bevan R.D., P.C., Pegram B.L, R.E., Su C. Analysis of changes in reactivity of rabbit arteries and veins two weeks after induction of hypertension by coarctation of the abdominal aorta. Circ. Res. 37: 183, 1975.PubMedCrossRefGoogle Scholar
  15. 15.
    Berman M. Compartmental analysis in kinetics. In: Stacy R.W., Waxman B.D. (Eds.), Computers in Biomedical Research. Academic Press, New York, 1965, vol. 2, p. 173.Google Scholar
  16. 16.
    Llaurado J.G. Relationship between kinetics of inflow and outflow as the basis of a computer simulation for solving compartmental models: example of electrolyte transfers in cardiovascular tissues. In: Dynamic Studies with Radioisotopes in Medicine, International Atomic Energy Agency, Vienna, 1971, pp. 13–26.Google Scholar
  17. 17.
    Gunn R.B., Patlak C.S. The uptake curve in tracer kinetics. Math. Biosci. 6: 19, 1970.CrossRefGoogle Scholar
  18. 18.
    Villamil M.F., Rettori V., Barajas L., Kleeman C.R. Extracellular space and the ionic distribution in the isolated arterial wall. Amer. J. Physiol. 214: 1104, 1968.PubMedGoogle Scholar
  19. 19.
    Friedman S.M., Friedman C.L. Cell permeability, sodium transport, and the hypertensive process in rat. Circ. Res. 39: 433, 1976.PubMedCrossRefGoogle Scholar
  20. 20.
    Altman J., Garay R., Papadimitriou A., Worcel M. Alterations in 22Na fluxes of arterial smooth muscles of spontaneously hypertensive rats. Br. J. Pharmacol. 59: 496P, 1977.PubMedCentralPubMedCrossRefGoogle Scholar
  21. 21.
    Llaurado J.G., Madden J.A, Meade R.C., Smith G.A. Distribution of thallium-201 injected into rats following stress: imaging, organ to plasma uptake ratios, and myocardial kinetics. J. Nucl. Med. 19: 172, 1978.PubMedGoogle Scholar
  22. 22.
    Gabella G. Caveolae intracellulars and sarcoplasmic reticulum in smooth muscle. J. Cell. Sci. 8: 601, 1971.PubMedGoogle Scholar
  23. 23.
    Woodbury D.M., Koch A. Effects of aldosterone and desoxycorticosterone on tissue electrolytes. Proc. Soc. Exp. Biol. Med. 94: 720, 1957.PubMedCrossRefGoogle Scholar
  24. 24.
    French, I.W., Manery J.F. The effect of aldosterone on electrolytes in muscle, kidney cortex, and serum. Can. J. Biochem. 42: 1459, 1964.PubMedCrossRefGoogle Scholar
  25. 25.
    Losert W., Senft C., Senft G. Extrarenale Wirkungen der Aldosterone und der Spirolactone. Arch. Exp. Path. Pharmak. 248: 450, 1964.CrossRefGoogle Scholar
  26. 26.
    Richards P., Smith K., Metcalfe-Gibson A., Wrong O. Action of d-aldosterone on the electrolyte composition of human cells grown in vitro. Lancet 2: 1099, 1966.PubMedCrossRefGoogle Scholar
  27. 27.
    Gross F., Schmidt H. Aldosterone overdosage in the rabbit. Acta Endocrinol. (Kbh) 28: 467, 1958.Google Scholar
  28. 28.
    Dawborn J.K., Ross E.J. The effect of prolonged administration of aldosterone on sodium and potassium turnover in the rabbit. Clin. Sci. 32: 559, 1967.PubMedGoogle Scholar
  29. 29.
    Robb C.A., Davis J.O., Johnston C.I., Hartroft P.M. Effects of cortisone on renal sodium excretion in rabbits. Endocrinology 82: 1200, 1968.PubMedCrossRefGoogle Scholar
  30. 30.
    Braverman B., Davis J.O. Adrenal steroid secretion in the rabbit: sodium depletion, angiotensin II, and ACTH. Amer. J. Physiol. 225: 1306, 1973.PubMedGoogle Scholar
  31. 31.
    Williams R.J.P., Wacker W.E.C. Cation balance in biological systems. J.A.M.A. 201: 18, 1967.CrossRefGoogle Scholar

Copyright information

© Italian Society of Endocrinology (SIE) 1978

Authors and Affiliations

  • J. G. Llaurado
    • 1
  • G. A. Smith
    • 1
  1. 1.Nuclear Medicine Service and Biomedical Engineering Group, Wood Veterans Administration CenterMarquette University and Medical College of WisconsinMilwaukeeUSA

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