Journal of Endocrinological Investigation

, Volume 1, Issue 2, pp 163–166 | Cite as

A rapid semi-automated method of determining total estrogens in non-pregnancy urine

  • David O. E. Gebhardt
  • Willem Soederhuizen
  • Abraham J. Moolenaar


A rapid routine method of determining estrogens in non-pregnancy urine is described. It involves the following steps: 1) Dilution of each urine sample with an equal volume of saturated ammonium sulfate solution; 2) Addition of Sephadex G-15 beads; 3) Filtration of the mixture; 4) Liberation of the estrogens from the Sephadex by washing the beads on the filter with 0.1N NaOH; 5) Automated fluorimetric analysis of the alkaline filtrate according to the procedure used for the determination of estrogen in pregnancy urine. A correction for non-specific fluorescence remains necessary and is achieved by measuring background fluorescence with a second fluorimeter.


Automated estrogen determination method of estrogen determination non-pregnancy estrogen determination urinary estrogen content estrogen content 


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Copyright information

© Italian Society of Endocrinology (SIE) 1978

Authors and Affiliations

  • David O. E. Gebhardt
    • 1
  • Willem Soederhuizen
    • 1
  • Abraham J. Moolenaar
    • 1
  1. 1.Department of Obstetrics and Gynecology and Department of Pathological ChemistryUniversity HospitalLeidenThe Netherlands

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