Journal of Endocrinological Investigation

, Volume 12, Issue 4, pp 279–293 | Cite as

Somatomedins: Chemical and functional characteristics of the different molecular forms

  • A. Barreca
  • F. Minuto
Review Article


Insulin-like growth factors somatomedins chemical structure molecular forms binding proteins receptors 


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  1. 1.
    Murphy W.R., Daughaday W.H., Hartnett C. The effect of hypophysectomy and growth hormone in the incorporation of labelled sulphate into tibial epiphyseal and nasal cartilage of the rat. J. Lab. Clin. Med. 47: 715, 1956.PubMedGoogle Scholar
  2. 2.
    Salmon W.D. Jr., Daughaday W.H. A hormonaliy controlled serum factor which stimulates sulfate incorporation by cartilage in vitro, J. Lab. Clin. Med. 49: 825, 1957.PubMedGoogle Scholar
  3. 3.
    Daughaday W.H., Hall K., Raben M.S., Salmon W.D. Jr., Van Den Brande J.L., Van Wyk J.J. Somatomedin: Proposed designation for sulfation factor. Nature 235: 107, 1972.PubMedGoogle Scholar
  4. 4.
    Hall K. Human somatomedin: Determination, occurrence, biological activity and purification. Acta Endocrinol. Suppl. 10: 1, 1972.Google Scholar
  5. 5.
    Uthne K. Human somatomedins: Purification and studies on their biological actions. Acta Endocrinol. 73(suppl. 175): 1, 1973.Google Scholar
  6. 6.
    Van Wyk J.J., Underwood L.E., Hintz R.L., Clemmons D.R., Voina S.J., Weaver R.P. The somatomedins: A family of insulinlike hormones under growth hormone control. Rec. Progr. Horm. Res. 30: 259, 1974.PubMedGoogle Scholar
  7. 7.
    Fryklund L., Skottner A., Sievertsson H., Hall K. Somatomedins A and B: Isolation, chemistry and in vivo effects. In: Pecile A., Müller E.E. (Eds.), Growth hormone and related peptieds. Excerpta Medica, Amsterdam, 1976, p 156.Google Scholar
  8. 8.
    Heldin C.H. Somatomedin-B: Mitogenic activity derived from contaminant epidermal growth factor. Science 213: 422, 1981.Google Scholar
  9. 9.
    Froesch E.R., Bürgi H., Ramseier E.B., Bally P., Labhart A. Antibody suppressible and nonsuppressible insulin-like activities in human serum and their physiological significance. An insulin assay with adipose tissue of increased precision and specificity. J. Clin. Invest. 47: 1816, 1963.Google Scholar
  10. 10.
    Rinderknecht E., Humbel R.E. The amino acid sequence of insulin-like growth factor I and its structural homology with proinsulin. J. Biol. Chem. 253: 2769, 1978.PubMedGoogle Scholar
  11. 11.
    Rinderknecht E., Humbel R.E. Primary structure of human insulin-like growth factor-II. FEBS Lett. 89: 283, 1978.PubMedGoogle Scholar
  12. 12.
    Pierson R.W., Temin H.M. The partial purification from calf serum of a fraction with multiplication stimulating activity for chicken fibroblasts in cell culture and with non-suppressible insulin-like activity. J. Cell. Physiol. 79: 319, 1972.PubMedGoogle Scholar
  13. 13.
    Dulak N., Temin H.N. A partially purified polypeptide fraction from rat liver cell conditioned medium with multiplication -stimulating activity for embryo fibroblasts. J. Cell. Physiol. 81: 153, 1973.PubMedGoogle Scholar
  14. 14.
    Marquardt H., Todaro G.J., Henderson L.E., Oroszlan S. Purification and primary structure of a polypeptide with multiplicating-stimulating activity from rat liver cell cultures. J. Biol. Chem., 256: 6859, 1981.PubMedGoogle Scholar
  15. 15.
    Nissley S.P., Adams S.O., Acquaviva A.M., Yang Y.W.H., Bruni B., Angust G.P., White R.M., Foley T.F.Jr., Moses A.C., Cohen K.L., Rechler M.M. Multiplication stimulating activity for cells in culture. In: De Gruyter W. (Ed.), Insulin-like growth factors/- somatomedins: basic chemistry; biology; clinical importance. Spencer E.M., Berlin/NY, 1983, p. 31Google Scholar
  16. 16.
    Van Wyk J.J., Underwood LE. The somatomedins and their actions. In: Litwack G. (Ed.), Biochemical actions of hormones. Academic Press Inc., New York, 1978, vol. 5, p. 101.Google Scholar
  17. 17.
    Buergi H., Mueller W.A., Humbel R.E., Labhart A., Froesch E.R. Non-suppressible insulin-like activity of human serum. I. Physiochemical properties, extraction and partial purification. Biochim. Biophys. Acta 121: 349, 1966.Google Scholar
  18. 18.
    Van Wyk J.J., Hall K., Weaver R.P. Partial purification of sulphation factor and thymidine factor from plasma. Biochim. Biophys. Acta 192: 560, 1969.PubMedGoogle Scholar
  19. 19.
    Klapper D.G., Svoboda M.E., Van Wyk J.J. Sequence of somatomedin-C. Confirmation of identity with insulin-like growth factor-I. Endocrinology 112: 2215, 1983.PubMedGoogle Scholar
  20. 20.
    Spencer E.M., Ross M., Smith B. The identity of human insulin-like growth factors I and II with somatomedins C and A and omology with rat IGF-I and II. In: De Gruyter W. (Ed.), Insulin-like growth factors/- somatomedins: Basic chemistry, biology, clinical importance. Spencer E.M., Berlin/N.Y, 1983, p. 81.Google Scholar
  21. 21.
    Enberg G., Carlquist M., Jornvall H., Hall K. The characterization of somatomedin A, isolated by microcomputer-controlled chromatography, reveals an apparent identity to insulin-like growth factor I. Eur. J. Biochem. 143: 117, 1984.PubMedGoogle Scholar
  22. 22.
    Cockerill P.N., Cornell H.Y., Herington A.C. Partial purification of low molecular weight non-suppressible insulin-like activity from human plasma: Demonstration of the presence of multiple forms. J. Endocrinol. 85: 267, 1980.Google Scholar
  23. 23.
    Van Wyk J.J., Hall K., Van Den Brande J.L., Weaver R.P. Further purification and characterization of sulfation factor and thymidine factor from acromegalic plasma. J. Clin. Endocrinol. Metab. 32: 389, 1971.PubMedGoogle Scholar
  24. 24.
    Posner B.J., Guyda H.J., Corvol T., Rappaport R., Harley C., Goldstein S. Partial purification, characterization and assay of a slightly acidic insulin-like peptide (ILAs) from human plasma. J. Clin. Endocrinol. Metab. 47: 1240, 1978.PubMedGoogle Scholar
  25. 25.
    Herington A.C., Kuffer A.D. Evidence for proteolytic conversion of insulin-like growth factors to a biologically active acidic form. In: De Gruyter W. (Ed.), “Insulin-like growth factors/- somatomedins: Basic chemistry, biology, clinical importance”. Spencer E.M., Berlin/NY, 1983, p. 113.Google Scholar
  26. 26.
    Bala R.M., Blakeley E.D., Smith G.R. Size heterogeneity of human serum somatomedin. J. Clin. Endocrinol. Metab. 43: 1110, 1976.PubMedGoogle Scholar
  27. 27.
    Van Den Brande J.L., Hoogerbrugge C. Plasma fractions containing somatomedin activity. Part. I. Extraction and partial characterization. In: Pecile A., Müller E.E. (Eds.), Growth hormone and other biologically active peptides. Excerpta Medica, Amsterdam, 1980, p. 81.Google Scholar
  28. 28.
    Svoboda M.E., Van Wyk J.J., Klapper D.G., Fellows R.E., Grissom F.E., Schlueter R.J. Purification of somatomedin-C from human plasma: Chemical and biological properties, partial sequence analysis and relationship to other somatomedins. Biochemistry 19: 790, 1980.PubMedGoogle Scholar
  29. 29.
    Minuto F., Barreca A., Del Monte P., Giordano G. Partial purification and characterization of a neutral insulin-like growth factor. Biochemical and biological properties. Biochem. International 13: 253, 1986.Google Scholar
  30. 30.
    Jansen M., van Schaik F.M.A., van Tol H., Van den Brande J.L., Sussenbach J.S. Nucleotide sequence analysis of cDNAs encoding precursors of human insulin-like growth factors II (IGF-II)and an IGF-II variant. FEBSLett. 179: 243, 1985.Google Scholar
  31. 31.
    Zumstein P.P., Luethi C., Humbel R.E. Amino acid sequence of a variant proform of insulin-like growth factor II. Proc. Natl. Acad. Sci. USA 82: 3169, 1985.PubMedCentralPubMedGoogle Scholar
  32. 32.
    Haselbacher G., Humbel R.E. Evidence for two species of insulin-like growth factor II (IGF-II and “big” IGF-II) in human spinal fluid. Endocrinology 110: 1822, 1982.PubMedGoogle Scholar
  33. 33.
    Blundell T.L., Cutfield J.F., Cutfield S.M., Dodson E.J., Dodson G.G., Hodgkin D.C., Mercola D.A., Vijayan M. Atomic positions in thromboedral 2-zync insulin crystals. Nature 231: 506, 1971.PubMedGoogle Scholar
  34. 34.
    Blundell T.L., Bedakar S., Humbel R.E. Tertiary structures, receptor binding, and antigenicity of insulin-like growth factors. Fed. Proc. Fed. Am. Soc. Exp. Biol. 42: 2592, 1983.Google Scholar
  35. 35.
    Blundell T. Chemistry, structure and function of insulin and related hormones. FEBS Lett. 109: 167, 1980.PubMedGoogle Scholar
  36. 36.
    Bradshaw R.A., Niall H.D. Insulin-related growth factors. TIBS 3: 274, 1978.Google Scholar
  37. 37.
    Blundell T.L., Humbel R.E. Hormone families: Pancreatic hormones and homologous growth factors. Nature 287: 781, 1980.PubMedGoogle Scholar
  38. 38.
    Shapiro B., Pimstone B.L. A phylogenetic study on sulphation factor activity in 26 species. J. Endocrinol. 74: 129, 1977.PubMedGoogle Scholar
  39. 39.
    Dayhoff M.O. Atlas of protein sequence and structure. National Biochemical Research Foundation, 1978.Google Scholar
  40. 40.
    Daughaday W.H., Kapadia M., Yanow C.E., Fabrick K., Maritz I.K. Insulin-like growth factors I and II of non-mammalian sera. Gen. Comp. Endocrinol. 59: 316, 1985.PubMedGoogle Scholar
  41. 41.
    James R., Niall H., Kwok S., Bryant-Greenwood G. Primary structure of porcine refaxin: Homology with insulin and related growth factors. Nature 276: 544, 1977.Google Scholar
  42. 42.
    Bedakar S., Turnell W.G., Blundell T.L., Schwabe C. Relaxin as conformational homology with insulin. Nature 270: 449, 1977.Google Scholar
  43. 43.
    Schwabe C., McDonlad J.K., Steinetz B.G. Primary structure of the B-chain of porcine relaxin. Biochim. Biophys. Res. Commun. 73: 503, 1977.Google Scholar
  44. 44.
    Isaaks N., James R., Niall H., Bryant-Greenwood G., Dodson G., Evans A., North A.C.T. Relaxin and its structural relationship to insulin. Nature 271: 278, 1978.Google Scholar
  45. 45.
    Angeletti R.H., Bradshaw R.A. Nerve growth factor from mouse salivary gland amino acid sequence. Proc. Natl. Acad. Sci. USA 68: 2417, 1971.PubMedCentralPubMedGoogle Scholar
  46. 46.
    Bradshaw R.A. Nerve growth factor. Ann. Rev. Biochem. 47: 191, 1978.PubMedGoogle Scholar
  47. 47.
    Jansen M., Van Schaik F.M.A., Ricker A.T., Bullock B., Woods D.E., Gabbay K.H., Nussbaum A.L., Sussenbach J.S., Van Den Brande J.L. Sequence of cDNA encoding human insulin-like growth factor I precursor. Nature 306: 609, 1983.PubMedGoogle Scholar
  48. 48.
    Bell G.I., Merryweather J.P., Sanchez-Pescador R., Stempien M.M., Priestley L., Scott J., Rall L.B. Sequence of a cDNA clone encoding human pre-proinsulin-like growth factor II. Nature 310: 775, 1984.PubMedGoogle Scholar
  49. 49.
    Tricoli J.V., Rall L.B., Scott J., Bell G.I., Shows T.B. Localization of insulin-like growth factor genes to human chromosomes 11 and 12. Nature 310: 784, 1984.PubMedGoogle Scholar
  50. 50.
    Jansen M., De Pagter-Holthuizen P., Van den Brande J.L., Sussenbach J.S. Somatomedin gene structure and expression. In: Cristiansen C., Riis B.J. (Eds.), Highlights on Endocrinology”. Ist European Congress of Endocrinology, Copenhagen, June 21–25, 1987, 1988, p. 217.Google Scholar
  51. 51.
    Rotwein P., Pollock K.M., Didier D.K., Krivi G.G. Organization and sequence of the huyman insulin-like growth factor I gene. J. Biol. Chem. 261: 4828, 1986.PubMedGoogle Scholar
  52. 52.
    Zapf J., Schoenle E., Froesch E.R. Insulin-like growth factors I and II: Some biological actions and receptor binding characteristics of two purified constituents of non-suppresssible insulin-like activity of human serum. Eur. J. Biochem. 87: 285, 1978.PubMedGoogle Scholar
  53. 53.
    Clemmons D.R., Van Wyk J.J. Somatomedin-C and platelet-derived growth factor stimulate human fibroblast replication. J. Cell. Physiol. 106: 361, 1981.PubMedGoogle Scholar
  54. 54.
    Vetter U., Zapf J., Heit W., Helbing G., Heinze E., Froesch E.R., Teller W.M. Human fetal and adult chondrocytes. Effect of IGF-I and -II, insulin and GH on clonal growth. J. Clin. Invest. 77: 1903, 1986.Google Scholar
  55. 55.
    Van Buul-Offers S., Van Den Brande J.L. Plasma fractions containing somatomedin activity. Part II: biological effects in Snell dwarf mice. In: Pecile A., Mueller E.E. (Eds.), Growth hormone and other biologically active peptide. Excepta Medica, Amsterdam, 1980, p. 103.Google Scholar
  56. 56.
    Schoenle E., Zapf J., Froesch E.R. Insulin-like growth factor I stimulates growth in hypophysectomized rats. Nature 296: 252, 1982.PubMedGoogle Scholar
  57. 57.
    Schoenle E., Zapf J., Froesch E.R. Insulin-like growth factor I and II stimulate growth of hypophysectomized rats. Diabetologia 23: 199, 1982.Google Scholar
  58. 58.
    Van Buul-Offers S., Veda I., Van Den Brande J.L. Biosynthetic somatomedin-C (SM-C/IGF-I) increases the length ans weight of Snell dwarf mice. Pediatric Res. 20: 825, 1986.Google Scholar
  59. 59.
    Schoenle E., Zapf J., Hauri C., Steiner T., Froesch E.R. Comparison of in vivo effects of insulin-like growth factors I and II and of growth hormone in hypophysectomized rats. Acta Endocrinol. 108: 157, 1985.Google Scholar
  60. 60.
    Guler H.P., Zapf J., Froesch E.R. Short-term metabolic effects of recombinant human insulin-like growth factor I in healthy adults. N. Engl. J. Med. 317: 137, 1987.PubMedGoogle Scholar
  61. 61.
    Clemmons D.R., Klibanski A., Underwood LE., Mac Arthur J.W., Ridgway E.C., Beitins I.Z., Van Wyk J.J. Reduction of plasma immunoreactive somatomedin-C during fasting in humans. J. Clin. Endocrinol. Metab. 53: 1247, 1981.PubMedGoogle Scholar
  62. 62.
    Minuto F., Barreca A., Adami G.F., Fortini P., Del Monte P., Cella F., Scopinaro N., Giordano G. Insulin-like growth factor-I in human malnutrition: Relationship with some body composition and nutritional parameters. JPEN in press, 1989.Google Scholar
  63. 63.
    Daughaday W.H., Hall K., Salmon W.D., Van Den Brande J.L., Van Wyk J.J. Letter to the Editor: On the nomenclature of the somatomedins and insulin-like growth factors. J. Clin. Endocrinol. Metab. 65: 1075, 1987.PubMedGoogle Scholar
  64. 64.
    Zapf J., Walter H., Froesch E.R. Radioimmunological determination of insulin-like growth factors I and II in normal subjects and in patients with growth disorders and extrapancreatic tumor hypoglycemia. J. Clin. Invest. 68: 1321, 1981.PubMedCentralPubMedGoogle Scholar
  65. 65.
    Schoenle E., Zapf J., Froesch E.R. Long-term in vivo effects of insulin-like growth factors I and II on growth indices: direct evidence in favor of the somatomedin-hypothesis. In: De Gruyter W. (Ed.), Insulin-like growth factors/- somatomedins: basic chemistry; biology; clinical importance. Spencer E.M., Berlin/NY, 1983, p. 51.Google Scholar
  66. 66.
    Zapf J., Schmid C., Froesch E.R. Biological and immunological properties of insulin-like growth factors I and II. J. Clin. Endocrinol. Metab. 13: 3, 1984.Google Scholar
  67. 67.
    Underwood L.E., D’Ercole A.J. Insulin and Insulin-like growth factors/somatomedins in fetal and neonatal development. Clin. Endocrinol. Metab. 13: 69, 1984.PubMedGoogle Scholar
  68. 68.
    Bhaumich B., Bala R.M., Hollemberg M.D. Somatomedin receptor of human placenta: Solubilization, photolabeling, partial purification and comparison with insulin receptor. Proc. Natl. Acad. Sci. USA 78: 4278, 1981.Google Scholar
  69. 69.
    Kasuga M., Van Obbergen E., Nissley S.P., Rechler M.M. Demonstration of two subtypes of insulin-like growth factor receptors by affinity cross-linking. J. Biol. Chem. 256: 5305, 1981.PubMedGoogle Scholar
  70. 70.
    Chernausek S.D., Jacobs S., Van Wyk J.J. Structural similarities between human receptors for somatomedin C and insulin: Analysis by affinity labeling. Biochemistry 20: 7345, 1981.PubMedGoogle Scholar
  71. 71.
    Massague J., Czeck M.P. The subunit structure of two distinct receptors for insulin-like growth factors I and II and their relationship to the insulin receptor. J. Biol. Chem. 257: 5038, 1982.PubMedGoogle Scholar
  72. 72.
    Jacobs S., Kull F.C., Cuatrecasas P. Monensin blocks the maturation of receptors for insulin and somatomedin C: Identification of receptors precursor. Proc. Natl. Acad. Sci. U.S.A. 80: 1228, 1983.PubMedCentralPubMedGoogle Scholar
  73. 73.
    Oppenheimer C.L., Czech M.P. Purification of the type II insulin-like growth factor receptor from rat placenta. J. Biol. Chem. 258: 8539, 1983.PubMedGoogle Scholar
  74. 74.
    August G.P., Nissley S.P., Kasuga M., Lee L., Greenstein L., Rechler M.M. Purification of an insulin-like growth factor II receptor from rat chondrosarcoma cell. J. Biol. Chem. 258: 9033, 1983.PubMedGoogle Scholar
  75. 75.
    Perdue J.F. Chemistry, structure and function of insulin-like growth factors and their receptors: A review. Can. J. Biochem. Cell. Biol. 62: 1237, 1984.PubMedGoogle Scholar
  76. 76.
    Hintz R.L., Clemmons D.R., Underwood L.E., Van Wyk J.J. Competitive binding of somatomedins to the insulin receptors of adipocytes, chondrocytes and liver membranes. Proc. Natl. Acad. Sci. USA 69: 2351, 1972.PubMedCentralPubMedGoogle Scholar
  77. 77.
    Daughaday W.H., Kipnis D.M. Growth promoting and anti insulin actions of somatotropin. Rec. Progr. Horm. Res. 22: 49, 1966.PubMedGoogle Scholar
  78. 78.
    Van Den-Brande J.L., Van Wyk J.J., Weaver R.P., Mayberry H.E. Partial characterization of sulfation and thymidine factors in acromegalic plasma. Acta Endocrinol. 66: 65, 1971.PubMedGoogle Scholar
  79. 79.
    Kapf J., Waldvogel M., Froesch E.R. Binding of non-suppressible insulin-like activity to human serum. Evidence for a carrier protein. Arch. Biochem. Biophys. 168: 638, 1975.Google Scholar
  80. 80.
    Hintz R.L., Liu F. Demonstration of specific plasma protein binding sites for somatomedin. J. Clin. Endocrinol. Metab. 45: 988, 1977.PubMedGoogle Scholar
  81. 81.
    Zapf J., Schoenle E., Jagars G., Sand I., Grunwald J., Froesch E.R. Inhibition of the action of nonsuppressible insulin-like activity on isolated rat fat cells by binding to its carrier protein. J. Clin. Invest. 63: 1077, 1979.PubMedCentralPubMedGoogle Scholar
  82. 82.
    Cohen K.L., Nissley S.P. The serum half-life of somatomedin activity: Evidence for growth hormone dependence. Acta Endocrinol. 83: 243, 1976.PubMedGoogle Scholar
  83. 83.
    Moses A.C., Nissley S.P., Cohen K.L., Rechler M.M. Specific binding of a somatomedin-like polypeptide in rat serum depends on growth hormone. Nature 263: 137, 1976.PubMedGoogle Scholar
  84. 84.
    White R.M., Nissley S.P., Moses A.C., Rechler M.M., Johnsonbaugh R.E. The growth hormone dependence of a serum somatomedin-binding protein in human serum. J. Clin. Endocrinol. Metab. 53: 49, 1981.PubMedGoogle Scholar
  85. 85.
    Furlanetto R.W. The somatomedin C binding protein: Evidence for a heterologous subunit structure. J. Clin. Endocrinol. Metab. 51: 12, 1980.PubMedGoogle Scholar
  86. 86.
    Borsi L., Rosenfeld R.G., Liu F., Hintz R.L. Somatomedin peptide distribution and somatomedin-binding protein content in cord plasma: Comparison to normal and hypopituitary plasma. J. Endocrinol. Metab. 54: 223, 1982.Google Scholar
  87. 87.
    Rieu M., Girard F., Bricaire M., Binoux M. The importance of insulin-like growth factor (somatomedin) measurements in the diagnosis and surveillance of acromegaly. J. Clin. Endocrinol. Metab. 55: 147, 1982.PubMedGoogle Scholar
  88. 88.
    Binoux M., Hardouin S., Lassarre C., Hossenlopp P. Evidence for production by the liver of two IGF binding proteins with similar molecular weights but different affinities for IGF-I and IGF-II. Their relations with serum and cerebrospinal fluid IGF binding proteins. J. Clin. Endocrinol. Metab. 55: 600, 1982.Google Scholar
  89. 89.
    Hintz R.L., Liu F. Human somatomedin plasma binding proteins. In: Giordano G., Van Wyk J.J., Minuto F. (Eds.), Somatomedins and growth. Serono Symposia n. 23. Academic Press. Inc., London, 1979, p. 143.Google Scholar
  90. 90.
    Froesch E.R., Zapf J., Walter H., Schoenle E., Rinderknecht E., Humbel R.E. Serum NSILA and IGF: News and riddles. In: Dumont J., Nunez J. (Eds.), Hormones and cell regulation. Elsevier/North Holland, Amsterdam, 1979, vol. 3, p. 149.Google Scholar
  91. 91.
    Barreca A., Hoogerbrugge C.M., van Buul-Offers S.C., Minuto F., Van Den Brande J.L. Somatomedin activity and binding characteristics of the 150 and 60 kDa complexes. Involvement of serum and cartilage proteases (in preparation).Google Scholar
  92. 92.
    Chatelain P.G., Van Wyk J.J., Copeland K.C., Blethen S.L., Underwood LE. Effect of “in vitro” action of serum proteases or exposure to acid on measurable immunoreactive somatomedin-C in serum. J. Clin. Endocrinol. Metab. 56: 376, 1983.PubMedGoogle Scholar
  93. 93.
    Clemmons D.R., Underwood L.E., Chatelain P.G., Van Wyk J.J. Liberation of immunoreactive somatomedin-C from its binding proteins by proteolytic enzymes and heparin. J. Clin. Endocrinol. Metab. 56: 384, 1983.PubMedGoogle Scholar
  94. 94.
    Hall K., Brandt J., Engberg G., Fryklund L. Immunoreactive somatomedin A in human serum. J. Clin. Endocrinol. Metab. 48: 271, 1979.PubMedGoogle Scholar
  95. 95.
    Wilkins J.R., D’Ercole A.J. Affinity labelled plasma somatomedin-C/insulin like growth factor I binding proteins. Evidence of growth hormone dependence and subunits structure. J. Clin. Invest. 75: 1350, 1985.Google Scholar
  96. 96.
    Binoux M., Hossenlop P., Hardouin S., Seurin D., Lassarre C., Gourmelen M. Somatomedin (Insulin-like Growth Factors) — binding proteins. Molecular forms and regulation. Horm. Res. 24: 141, 1986.Google Scholar
  97. 97.
    Grant M.B., Russel B., Harwood H.J., Merimee T.J. Separation of the insulin-like growth factor-binding proteins in plasma and purification of the large molecular weight species. J. Clin. Endocrinol. Metab. 64: 1060, 1987.PubMedGoogle Scholar
  98. 98.
    Barreca A., Del Monte P., Fortini P., Cariola G., Cella F., Minuto F. Sm-C/IGF-I binding proteins: Role of the acid labile component of the 150 K complex. J. Endocrinol. Invest. 10(Suppl. 1): 53, 1987.Google Scholar
  99. 99.
    Martin L.J., Baxter R.C. Antibody against acid-stable insulin-like growth factor binding protein detects 150, 000 mol wt growth hormone dependent complex in human plasma. J. Clin. Endocrinol. Metab. 61: 799, 1985.PubMedGoogle Scholar
  100. 100.
    Baxter R.C. Characterization of the acid-labile subunit of the growth hormone dependent insulin-like growth factor binding protein complex. J. Clin. Endocrinol. Metab. 67: 265, 1988.PubMedGoogle Scholar
  101. 101.
    Salmon W.D. Jr., Duvall M.R. A serum fraction with “sulfation factor activity” stimulates in vitro incorporation of leucine and sulfate into protein-polysaccaride complexes, uridine into RNA and thymidine into DNA of costal cartilage from hypophysectomized rats. Endocrinology 86: 721, 1970.PubMedGoogle Scholar
  102. 102.
    Daughaday W.H., Mariz I.K. Conversion of prolyne U-C14 to labeled hydroxyproline by rat cartilage in vitro: Effects of hypophysectomy, growth hormone and cortisol. J. Lab. Clin. Med. 59: 741, 1962.PubMedGoogle Scholar
  103. 103.
    Garland J.T., Lottes M.E., Kozak S., Daughaday W.H. Stimulation of DNA synthesis in isolated chondrocyted by sulfation factor. Endocrinology 90: 1086, 1972.PubMedGoogle Scholar
  104. 104.
    Stiles C.D., Capone G.T., Scher C.D., Antoniades H.N., Van Wyk J.J., Pledger W.J. Dual control of cell growth by somatomedins and platelet derived growth factor. Proc. Natl. Acad. Sci. USA 76: 1279, 1979.PubMedCentralPubMedGoogle Scholar
  105. 105.
    Moses A.C., Nissley S.P., Short P.A., Rechler M.M., Podskanly J.M. Purification and characterization of multiplication stimulating activity. Eur. J. Biochem. 103: 387, 1980.PubMedGoogle Scholar
  106. 106.
    Florini J.R., Nicholson M.L., Dulak N.C. Effects of peptide anabolic hormones on growth of myoblasts in culture. Endocrinology 101: 32, 1977.PubMedGoogle Scholar
  107. 107.
    Rabinovitch A., Quigley C., Russel T., Patel Y., Mintz D.H. Insulin and multiplication-stimulating activity (insulin-like growth factor) stimulates islet beta-cells replication in neonatal rat pancreatic monolayer cultures. Diabetes 31: 160, 1982.PubMedGoogle Scholar
  108. 108.
    Schimpft R.M., Repellin A.M., Salvatori A., Thieriot-Prevost G., Chatelain P. Effect of purified somatomedins on thymidine incorporation into lectin-activated human lymphocytes. Acta Endocrinol. 102: 21, 1983.Google Scholar
  109. 109.
    Reddan J.R., Wilason-Dziedzic D. Insulin-like growth factor and epidermal growth factor trigger mitosis in lenses cultured in a serum-free medium. Invest. Visual Sci. 24: 409, 1983.Google Scholar
  110. 110.
    Lenoir D., Honegger P. Insulin-like growth factor I (IGF-I) stimulates DNA synthesis in fetal rat brain cell cultures. Brain Res. 283: 205, 1983.PubMedGoogle Scholar
  111. 111.
    Borland K., Mita M., Oppenheimer C.L., Blinderman LA., Massague J., Hall P.F., Czeck M.P. The actions of insulin-like growth factor I and II on cultured Sertoli cells. Endocrinology 114: 220, 1984.Google Scholar
  112. 112.
    Florini J.R., Ewton D.Z., Falen S.L., Van Wyk J.J. Biphasic concentration dependency of stimulation of myoblast differentiation by somatomedins. Am. J. Physiol. (Cell. Physiol. 19) 250: c1, 1986.Google Scholar
  113. 113.
    Beebe D.C., Silver M.H., Snellings K.L., Van Wyk J.J., Zelenka P.S. Lentropin, an inducer of lens fiber formation, is an insulin-like growth factor. Proc. Natl. Acad. Sci. USA, 1986.Google Scholar
  114. 114.
    Van Wyk J.J., Russell W.E., Underwood L.E., Svoboda M.E., Gillespie G.Y., Pledger U.J., Adashi E.Y., Balk S.D. Action of somatomedins on cell growth: Effect of selective neutralization of somatomedin-C (insulin-like growth factor I) with a monoclonal antibody. In: Raiti S., Tolman R. (Eds.), Human growth hormone. Plenum Publishing Corp., New York, 1986, p. 585.Google Scholar
  115. 115.
    Green H., Morikawa N., Nixon T. A dual effector theory of growth hormone action. Differentiation 29: 195, 1985.PubMedGoogle Scholar
  116. 116.
    Zezulak K.M., Green H. The generation of insulin-like growth factor I sensitive cells by growth hormone action. Science 233: 551, 1986.PubMedGoogle Scholar
  117. 117.
    Adashi E.Y., Resnik C.E., Svoboda M.E., Van Wyk J.J. Somatomedin-C synergises with follicle stimulating hormone in the acquisition of progestin biosynthetic capacity by cultured rat granulosa cells. Endocrinology 116: 2135, 1985.PubMedGoogle Scholar
  118. 118.
    Adashi E.Y., Resnick C.E., Svoboda M.E., Van Wyk J.J. Somatomedin-C enhances induction of luteinizing hormone receptors by follicle stimulating hormone in cultured rat granulosa cells. Endocrinology 116: 2369, 1985.PubMedGoogle Scholar
  119. 119.
    Adashi E.Y., Resnik C.E., Brodie A.M.H., Svoboda M.E., Van Wyk J.J. Somatomedin-C mediated potentiation of follicle stimulating hormone-induced aromatase activity of cultured rat granulosa cells. Endocrinology 117: 2313, 1985.PubMedGoogle Scholar
  120. 120.
    Adashr E.Y., Resnick C.E., Svoboda M.E., Van Wyk J.J. Somatomedin-C as an amplifier of follicle-stimulating hormone action: Enhanced accumulation of adeno-sine 3′5′-cyclic monophosphate. Endocrinology 118: 149, 1986.Google Scholar
  121. 121.
    Tramontane D., Cushing G.W., Moses A.C., Ingbar S.H. Insulin-like growth factor-I stimulates the growth of rat thyroid cells in culture and synergizes the stimulation of DNA synthesis induced by TSH and Graves’-IgG. Endocrinology 119: 940, 1986.Google Scholar
  122. 122.
    Smith P., Wynford-Thomas D., Stringer M.J., Williams E.D. Growth factor control of rat thyroid follicular cell proliferation. Endocrinology 119: 1439, 1986.PubMedGoogle Scholar
  123. 123.
    Minuto F., Barreca A., Del Monte P., Cariola G., Torre G.C., Giordano G. Immunoreactive insulin-like growth factor I (IGF-I) and IGF-I binding protein content in human thyroid tissue. J. Clin. Endocrinol. Metab. (in press), 1989.Google Scholar
  124. 124.
    Schalch D.S., Heinrich U.E., Draznin B., Johnson C.J., Miller L.L. Role of the liver in regulating somatomedin activity: Hormonal effects on the synthesis and release of insulin-like growth factor and its carrier protein by the isolated perfused rat liver. Endocrinology 104: 1143, 1979.PubMedGoogle Scholar
  125. 125.
    Spencer E.M. Synthesis by cultured hepatocytes of somatomedin and its binding protein. FEBSLett. 99: 157, 1979.Google Scholar
  126. 126.
    Scott C.D., Martin J.L., Baxter R.C. Production of insulin-like growth factor-I and its binding protein by adult rat hepatocytes in primary cultures. Endocrinology 116: 1094, 1985.PubMedGoogle Scholar
  127. 127.
    Atkinson P.R., Weidman E.R., Bhaumick B., Bala R.M. Release of somatomedin-like activity by cultured Wi-38 human fibroblasts. Endocrinology 106: 2006, 1980.Google Scholar
  128. 128.
    Clemmons D.R., Underwood LE., Van Wyk J.J. Hormonal control of immunoreactive somatomedin production by cultured fibroblasts. J. Clin. Invest. 67: 10, 1981.PubMedCentralPubMedGoogle Scholar
  129. 129.
    Clemmons D.R. Multiple hormones stimulates the production of somatomedin by cultured human fibroblasts. J. Clin. Endocrinol. Metab. 58: 850, 1984.PubMedGoogle Scholar
  130. 130.
    Hall K., Ritzen E.M., Johnsonbaugh R.E., Parvinen M. Pubertal rise of immunoreactive somatomedin and its eventual source. In: Gruyter W. (Ed.), Insulin-like growth factors/- somatomedins. Basic chemistry, biology, clinical importance. Spencer E.M. Ed., Berlin/NY, 1983, p. 611.Google Scholar
  131. 131.
    D’Ercole A.J., Applewhite G.T., Underwood L.E. Evidence that somatomedin is synthesized by multiple tissues in the fetus. Dev.Biol. 75: 315, 1980.PubMedGoogle Scholar
  132. 132.
    D’Ercole A.J., Stiles A.D., Underwood L.E. Tissue concentrations of somatomedin C: Further evidence for multiple sites of synthesis and paracrine or autocrine mechanisms of action. Proc. Natl. Acad. Sci. USA 81: 935, 1984.PubMedCentralPubMedGoogle Scholar
  133. 133.
    Kawai K., Takano K., Hizuka N., Shizume K. Properties and concentrations of somatomedin A in various rat tissues. Endocrinol. Jpn. 26: 559, 1979.PubMedGoogle Scholar
  134. 134.
    Sara V.R., Uvunas-Moberg K., Uvunas B., Hall K., Wetteberg L., Posloncec B., Goiny M. The distribution of somatomedins in the nervous system of the cat and their release following neural stimulation. Acta Physiol. Scand. 115: 467, 1982.PubMedGoogle Scholar
  135. 135.
    Acquaviva A.M., Bruni C.B., Nissley S.P., Rechler M.M. Cell-free synthesis of rat insulin-like growth factor II. Diabetes 31: 656, 1982.PubMedGoogle Scholar
  136. 136.
    Atkinson P.R., Bala R.M. Partial characterization of a mitogenic factor with somatomedin-like activity produced by culture WI-38 human fibroblasts. J. Cell. Physiol. 107: 317, 1981.Google Scholar
  137. 137.
    Clemmons D.R., Shaw D.S. Purification and biologic properties of fibroblast somatomedin. J. Biol. Chem. 261: 10293, 1986.PubMedGoogle Scholar
  138. 138.
    Moses A.C., Nissley S.P., Passamani J., White R.M., Rechler M.M. Further characterization of growth hormone-dependent somatomedin-binding proteins in rat serum and demonstration of somatomedin-binding proteins produced by rat liver cells in culture. Endocrinology 104: 536, 1979.PubMedGoogle Scholar
  139. 139.
    Binoux M., Hossenlop P., Lassarre C., Hardouin M. Production of insulin-like growth factors and their carrier by rat pituitary gland and brain expiants in culture. FEBS Lett. 124: 178, 1981.PubMedGoogle Scholar
  140. 140.
    Romanus J.A., Rabinovitch A., Rechler M.M. Neonatal rat islet cell cultures synthesize insulin-like growth factor I. Diabetes 34: 696, 1985.PubMedGoogle Scholar
  141. 141.
    Hill DJ., Frazer A., Swenne I., Wirdnam P.K., Milner R.D.G. Somatomedin-C in human fetal pancreas. Cellular localization and release during organ culture. Diabetes 36: 465, 1987.Google Scholar
  142. 142.
    Adams S.O., Kapadia M., Mills B., Daughday W.A. Release of insulin-like growth factors and binding protein activity into serum free medium of cultured human fibroblasts. Endocrinology 115: 520, 1984.PubMedGoogle Scholar
  143. 143.
    De Larco J.E., Todaro G.J. A human fibrosarcoma cell line producing multiplication stimulating activity (MSA)-related peptides. Nature 278: 356, 1978.Google Scholar
  144. 144.
    Anderson W.B., Nagarajan L., Jetten A., Rechler M.M., Nissley S.P. Production of insulin-like growth factor by DIFS endoderm cells: Possible early embryonic growth hormone. Cell. Biol. Int. Rep. 7: 563, 1983.Google Scholar
  145. 145.
    Minuto F., Del Monte P., Barreca A., Alama A., Cariola G., Giordano G. Evidence for autocrine mitogenic stimulation by somatomedin-C/insulin-like growth factor I on an established human lung cancer cell line. Cancer Res. 48: 3716, 1988.PubMedGoogle Scholar
  146. 146.
    Minuto F., Del Monte P., Barreca A., Nicolin A., Giordano G. Partial characterization of somatomedin C-like immunoreactivity secreted by breast cancer cells in vitro. Mol. Cell. Endocrinol. 54: 179, 1987.PubMedGoogle Scholar
  147. 147.
    Moses A.C., Pilistine S.J. Insulin-like growth factors. Control Anim. Cell. Prolif. 1: 91, 1985.Google Scholar
  148. 148.
    Morris D.H., Schalch D.S., Monty-Miles B. Identification of a somatomedin binding protein produced by a rat hepatoma cell line. FEBS Lett. 127: 221, 1981.PubMedGoogle Scholar
  149. 149.
    Moses A.C., Freinklen A.J., Knowles B.B., Aden D.P. Demonstration that a human hepatoma cell line produces a specific insulin-like growth factor carrier protein. J. Clin. Endocrinol. Metab. 56: 1003, 1983.PubMedGoogle Scholar
  150. 150.
    Baxter R.C., Maitland J.E., Raison R.L., Reddel R.R., Sutherland R.L. High-molecular weight somatomedin-C/IGF-I from T47D human mammary carcinoma cells: Immuno-reactivity and bioactivity. In: De Gruyter W. (Ed.), Insulin-like growth factors/- somatomedins, basic chemistry, biology, clinicai importance. Spencer E.M., Beriin/NY, 1983, p. 615.Google Scholar
  151. 151.
    Schwander J.C., Hauri C., Zapf J., Froesch E.R. Synthesis and secretion of insulin-like growth factor and its binding protein by perfused rat liver: Dependence on growth hormone status. Endocrinology 113: 297, 1983.PubMedGoogle Scholar
  152. 152.
    Kaufmann U., Zapf J., Froesch E.R. Growth hormone dependence of nonsuppressible insulin-like activity (NSILA) and of NSILA-carrier protein in rats. Acta Endocrinol. 87: 716, 1978.PubMedGoogle Scholar
  153. 153.
    Binoux M., Lassarre C., Hardouin N. Somatomedin production by rat liver in organ culture: III. Studies on the release of insulin-like growth factor and its carrier protein measured by radioligand assays. Effects of growth hormone, insulin and cortisol. Acta Endocrinol. 99: 422, 1982.Google Scholar
  154. 154.
    Barreca A., Del Monte P., Vargiu I., Cariola G., Minuto F. Characterization of the IGF-I carrier proteins produced by normal and neoplastic cells. J. Endocrinol. Invest. 11(Suppl. 2): 109, 1988.Google Scholar
  155. 155.
    Barreca A., Del Monte P., Giordano G., Minuto F. Generation of the 150 kDa IGF-I-binding protein complex from a fibroblast derived binding component and a serum derived acid labile component (in preparation).Google Scholar
  156. 156.
    Tramontano D., Cushing G.W., Moses A.C., Ingbar S.H. Insulin-like growth factor I stimulates the growth of rat thyroid cells in culture and synergizes the stimulation of DNA synthesis induced by TSH and Graves’-IgG. Endocrinology 119: 940, 1986.PubMedGoogle Scholar
  157. 157.
    Sporn M.B., Todaro G.J. Autocrine secretion and malignant transformation of cells. N. Engl.J. Med. 303: 878, 1980.PubMedGoogle Scholar
  158. 158.
    Todaro G.J., De Larco J.E. Growth factors produced by sarcoma virus-transformed cells. Cancer Res. 38: 4147, 1978.PubMedGoogle Scholar
  159. 159.
    Minuto F., Del Monte P., Barreca A., Fortini P., Cariola G., Catrambone G., Giordano G. Evidence for an increased somatomedin-C/insulin-like growth factor I content in primary human lung tumors. Cancer Res. 46: 985, 1986.PubMedGoogle Scholar
  160. 160.
    Huff K.K., Kaufmann D., Gabbay K.H., Spencer E.M., Lippmann M.E., Dickson R.B. Secretion of an insulin-like growth factor-I-related protein by human breast cancer cells. Cancer Res. 46: 4613, 1986.PubMedGoogle Scholar
  161. 161.
    Chochinov R.H., Mariz I.K., Hajek A.S., Daughday W.H. Characterization of a protein in mid-term human amniotic fluid which reacts in the somatomedin-C radioreceptor assay. J. Clin. Endocrinol. Metab. 44: 902, 1977.PubMedGoogle Scholar
  162. 162.
    Drop S.L.S., Valiquette G., Guyda H.J., Corvol M.T., Posner B.I. Partial purification and characterization of a binding protein for ILAs in human amniotic fluid: A possible inhibitor of insulin-like activity. Acta Endocrinol. 90: 505, 1979.PubMedGoogle Scholar
  163. 163.
    Povoa G., Enberg G., Jornvall H., Hall K. Isolation and characterization of a somatomedin-binding protein from midterm human amniotic fluid. Eur. J. Biochem. 144: 199, 1984.PubMedGoogle Scholar
  164. 164.
    Drop S.L.S., Kortleve D.J., Guyda H.J., Posner B.J. Immunoassay of a somatomedin-binding protein from human amniotic fluid: Levels in fetal, neonatal and adult sera. J. Clin. Endocrinol. Metab. 59: 908, 1984.PubMedGoogle Scholar
  165. 165.
    Povoa G., Roovete A., Hall K. Cross-reaction of serum somatomedin-binding protein in a radioimmunoassay developed for somatomedin-binding protein isolated from human amniotic fluid. Acta Endocrinol. 107: 563, 1984.PubMedGoogle Scholar
  166. 166.
    Koistinen R., Kalkkinen N., Huhtala M.L., Seppala M., Bohn H., Rutanen E.M. Placental protein 12 is a decidual protein that binds somatomedin ad has an identical N-terminal amino acid sequence with somatomedin-binding protein from human amniotic fluid. Endocrinology 118: 1375, 1986.PubMedGoogle Scholar
  167. 167.
    Povoa G., Isaksson M., Jornvall H., Hall K. The somatomedin-binding protein isolated from a human hepatoma cell line is identical to human-amniotic fluid somatomedin-binding protein. Res. Commun. 128: 1071, 1985.Google Scholar
  168. 168.
    Rutaten E.M., Wahlstrom T., Koistinen R., Sipponen P., Jalanko H., Seppala M. Placental protein 12 (PP12) in primary liver cancer and cyrrhosis. Tumor Biol. 5: 95, 1984.Google Scholar

Copyright information

© Italian Society of Endocrinology (SIE) 1989

Authors and Affiliations

  • A. Barreca
    • 1
  • F. Minuto
    • 2
  1. 1.Cattedra di Endocrinologia, ISMIUniversità di GenovaGenovaItaly
  2. 2.Fisiopatologia EndocrinaISMI, University of GenovaItaly

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