Journal of Endocrinological Investigation

, Volume 12, Issue 1, pp 25–29 | Cite as

Calcitonin suppresses growth hormone (GH) response to growth hormone — releasing hormone (GHRH) in man

  • Ana-Maria J. Lengyel
  • A. L. G. Toledo
  • M. A. Czepielewski
  • J. G. H. Vieira
  • A. R. Chacra


Calcitonin (CT) receptors have been found in the hypothalamus, suggesting a neuroendocrine role for this peptide. We have recently shown that, in the rat, central administration of salmon calcitonin (sCT) suppresses basal and GHRH-stimulated GH secretion. To further investigate how sCT alters GH secretion, we studied the effects of sCT (100U MRC, im) or placebo on basal and GHRH (50μg, iv)-stimulated GH secretion in 6 normal men. GHRH was administered 1 h after sCT injection. Basal GH levels were not altered by sCT administration. However, GH response to GHRH was markedly suppressed by sCT (area under the curve — sCT: 574.6 ± 69.7 vs placebo: 1057.2 ± 284.8 μg. min/L; p < 0.02). Cortisol levels were higher in sCT-treated subjects compared to controls, from 45 to 105 min after sCT injection (p < 0.05). However, no correlation was found between GH response to GHRH and Cortisol levels. No changes in glucose, calcium and PTH levels were seen. These results demonstrate that sCT inhibits GHRH-induced GH secretion in man by a mechanism apparently independent of changes in peripheral Cortisol, glucose, calcium and PTH levels.


Calcitonin GH GHRH mechanism suppression neuroendocrine 


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  1. 1.
    Leicht E., Biro G., Weinges K.F. Inhibition of releasing-hormone-induced secretion of TSH and LH by calcitonin. Horm. Metab. Res. 6: 410, 1974.PubMedCrossRefGoogle Scholar
  2. 2.
    Cantalamessa L., Catania A., Reschini E., Peracchi M. Inhibitory effect of calcitonin on growth hormone and insulin secretion in man. Metabolism, 27: 987, 1978.PubMedCrossRefGoogle Scholar
  3. 3.
    Petralito A., Lunetta M., Liuzzo A., Mangiafico P.A., Fiore C. Effects of salmon calcitonin on insulin-induced growth hormone release in man. Horm. Metab. Res. 11: 641, 1979.PubMedCrossRefGoogle Scholar
  4. 4.
    Isaac R., Merceron R., Caillens G., Raymond J.-P., Ardaillou R. Effect of calcitonin on basal and thyrotropin-releasing hormone-stimulated prolactin secretion in man. J. Clin. Endocrinol. Metab. 50: 1011, 1980.PubMedCrossRefGoogle Scholar
  5. 5.
    Olgiatti V.R., Guidobono F., Luisetto G., Netti C., Bianchi C., Pecile A. Calcitonin inhibition of physiological and stimulated prolactin secretion in rats. Life Sci. 29: 585, 1981.CrossRefGoogle Scholar
  6. 6.
    Mitsuma T., Nogimori T., Chaya M. Peripheral administration of eel calcitonin inhibits thyrotropin secretion in rats. Eur. J. Pharmacol. 102: 123, 1984.PubMedCrossRefGoogle Scholar
  7. 7.
    Tannenbaum G.S., Goltzman D. Calcitonin gene-related peptide mimics calcitonin action in brain on growth hormone release and feeding. Endocrinology 116: 2685, 1985.PubMedCrossRefGoogle Scholar
  8. 8.
    Minamitami N., Chihara K., Kaji M., Kodama H., Fujita T. Inhibitory effect of [Asu1, 7]-eel calcitonin on growth hormone secretion in conscious, freely moving, male rats. Endocrinology 117: 347, 1985.CrossRefGoogle Scholar
  9. 9.
    Fisher J.A., Tobler P.H., Kaufmann M., Born W., Henke H., Cooper P.E., Sagar S.M., Martin J.B. Calcitonin: regional distribution of the hormone and its binding sites in the human brain and pituitary. Proc. Natl. Acad. Sci. USA 78: 7801, 1981.CrossRefGoogle Scholar
  10. 10.
    Rizzo A.J., Goltzman D. Calcitonin receptors in the central nervous system of the rat. Endocrinology 108: 1672, 1981.PubMedCrossRefGoogle Scholar
  11. 11.
    Nakamuta H., Furakawa S., Koida M., Yajima H., Orlowski R.C., Schieuter R. Specific binding of 125l-salmon calcitonin to rat brain: regional variation and calcitonin specificity. Jpn. J. Pharmacol. 31: 53, 1981.PubMedCrossRefGoogle Scholar
  12. 12.
    Deftos L.J., Burton D., Catherwood B.D., Bone H.G., Parthermore J.G., Guillemin R., Watkins W.B., Moore R.Y. Demonstration by immunoperoxidase histochemistry of calcitonin in the anterior lobe of the rat pituitary. J. Clin. Endocrinol. Metab. 47: 457, 1978.PubMedCrossRefGoogle Scholar
  13. 13.
    Lengyel A.-MJ, Tannenbaum G.S. Mechanisms of calcitonin-induced growth hormone suppression: roles of somatostatin and GH-releasing factor. Endocrinology 120: 1377, 1987.PubMedCrossRefGoogle Scholar
  14. 14.
    Vieira J.G.H., Oliveira M.A.D., Maciel R.M.B., Mesquita C.H., Russo E.M.K. Development of an homologous radioimmunoassay for the synthetic amino terminal (1–34) fragment of human parathyroid hormone using egg yolk — obtained antibodies. J. Immunoassay 7: 57, 1986.PubMedCrossRefGoogle Scholar
  15. 15.
    Vieira J.G.H., Russo E.M.K., Germek O.A., Antunes L.N. Radioimmunoassay of serum Cortisol. Rev. Bras. Patol. Clin. 15: 125, 1979.Google Scholar
  16. 16.
    Ziegler R., Bellwinkel S., Schmidtchen D., Minne H. Effects of hypercalcemia, hypocalcemia and calcitonin on glucose stimulated insulin secretion in man. Horm. Metab. Res. 4: 60, 1972.PubMedCrossRefGoogle Scholar
  17. 17.
    Masuda A., Shibasaki T., Nakahara M., Imaki T., Kiyosawa Y., Jibiki K., Demura H., Shizume K., Ling N. The effects of glucose on growth hormone-releasing hormone-mediated GH secretion in man. J. Clin. Endocrinol. Metab. 60: 523, 1985.PubMedCrossRefGoogle Scholar
  18. 18.
    Press M, Tamborlane W.V., Thorner M.O., Vale W., Rivier J., Gertner J.M., Sherwin R.S. Pituitary response to growth hormone — releasing factor in diabetes. Failure of glucose — mediated suppression. Diabetes 33: 804, 1984.Google Scholar
  19. 19.
    Zofkova I, Hampl. R., Nedvidkova J. Effect of calcitonin on blood levels of glucose, insulin, somatotropin and Cortisol. Horm. Metab. Res. 16: 499, 1984.PubMedCrossRefGoogle Scholar
  20. 20.
    Laurian L., Oberman Z., Graf. E., Hoerer E., Simantov R. Calcitonin induced increase in ACTH, β-endorphin and Cortisol secretion. Horm. Metab. Res. 18: 268, 1986.PubMedCrossRefGoogle Scholar
  21. 21.
    Smals A.E.M., Pieters G.F.F.M., Smals AGH, Benraad Th.J., Kloppenborg P.W.C. Human pancreatic growth hormone releasing hormone fails to stimulate human growth hormone both in Cushing’s disease and in Cushing’s syndrome due to adrenocortical adenoma. Clin. Endocrinol. (Oxf.) 24: 401, 1986.CrossRefGoogle Scholar
  22. 22.
    Nakagawa K., Akikawa K., Matsubara M., Kubo M. Effect of dexamethasone on growth hormone response to growth hormone releasing hormone in acromegaly. J. Clin. Endocrinol. Metab. 60: 306, 1985.PubMedCrossRefGoogle Scholar
  23. 23.
    Minamitami N., Chihara K., Iwasaki J., Matsukura S., Fujita T. Attenuation by hypocalcemia of pulsatile growth hormone secretion in conscious male rats. Neuroendocrinology 35: 405, 1982.CrossRefGoogle Scholar
  24. 24.
    Reel J.R., Pastushok C.A., Vaitkus J.W., Sakowski R., Dermody W.C. Lack of a direct effect of salmon calcitonin on luteinizing hormone and thyrotropin release in anterior pituitary cell cultures. J. Endocrinol. 72: 99, 1977.PubMedCrossRefGoogle Scholar
  25. 25.
    Van Houten M., Rizzo A.J., Goltzman D., Posner B.I. Brain receptors for blood-borne calcitonin in rats: circumventricular localization and vasopressin-resistant deficiency in hereditary diabetes insipidus. Endocrinology 111: 704, 1982.CrossRefGoogle Scholar
  26. 26.
    Douglas W.W., Poisner A.M. Stimulus-secretion coupling in a neurosecretory organ: the role of calcium in the release of vasopressin from neurohypophysis. J. Physiol. (Lond) 172: 1, 1964.Google Scholar
  27. 27.
    Rubin R.P. The role of calcium in the release of neurotransmitter substances and hormones. Pharmacol. Rev. 22: 389, 1970.PubMedGoogle Scholar
  28. 28.
    Rasmussen H. Ionic and hormonal control of calcium homeostasis. Am. J. Med. 50: 567, 1971.PubMedCrossRefGoogle Scholar

Copyright information

© Italian Society of Endocrinology (SIE) 1989

Authors and Affiliations

  • Ana-Maria J. Lengyel
    • 1
  • A. L. G. Toledo
    • 1
  • M. A. Czepielewski
    • 1
  • J. G. H. Vieira
    • 1
  • A. R. Chacra
    • 1
  1. 1.Division of Endocrinology, Department of MedicineEscola Paulista de MedicinaSao PauloBrazil

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