Abstract
Human gonads were examined for the presence of ir growth hormone-releasing-hormone. We demonstrated the presence of immu-nostainable cells both in ovarian and in testicular tissue using a non-crossreactive antiGRH antiserum and the immunoperoxidase detection technique. In ovaries from ovulating women, GRH immunoreactivity was localized in the corpórea lutea; granulosa cells, theca cells and cells of primary follicles did not stain. In premenopausal ovaries, staining was detectable in scattered luteinized stromal cells. In testes from post-puberal men GRH immunoreactivity was localized in the Leyding cells; cells of the germinal epithelium did not stain. These results demonstrate the presence of GRH in human gonads and suggest that this peptide may exert regulatory function at the testicular and ovarian levels.
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Sharpe R.M. Intragonadal factors controlling testicular function. Biol. Reprod. 30:29, 1984.
Bardin C.W., Shaha C., Mather J., Salomon Y., Krieger D.T. Identification and possible function of pro-opiomelanocortin-induced peptides in the testis. Ann. NY Acad. Sci. 438: 346, 1984.
Fabbri A., Knox G., Buczlo E., Dufau M.L Beta-endorphin production by the fetal Leydig cell: regulation and implications for paracrine control of Sertoli cell function. Endocrinology 122: 749, 1988.
Yoon D.J., Sklar C., David R. Presence of immunoreactive corticotropin-releasing factor in rat testis. Endocrinology 122: 759, 1988.
Morley J.M., Meyer N., Pekary A.E., Melmed S., Carlton H.E., Briggs J.E., Hershman J.M. A prolactin inhibitory factor with immunocharacteristics similar to thyrotropin releasing factor (TRH) is present in rat pituitary tumors (GH3 and W5), testicular tissue and a plant material, alfalfa. Biochem. Biophys. Res. Comm. 96: 47, 1980.
Bhasin S., Heber D., Peterson M., Swerdloff R. Partial isolation and characterization of testicular GnRH-like factors. Endocrinology 112: 1144, 1983.
Pintar J.E., Schachter B.S., Herman A.B., Durgerian S., Krieger D.T. Characterization and localization of proopiomelanocortin messenger RNA in the adult rat testis. Science 225: 632, 1984.
Thorner M.O., Vance M.L., Evans W.S., Ho K., Rogol A.D., Blizzard R.M., Furlanetto R., Rivier L., Vale W. Growth hormone-releasing factor and somatomedin-C production: extra-hypothalamic localization and possible functional significance. Acta Endocrinol. (Copenh.) 276 (Suppl): 34, 1986.
Bruhn T.O., Mason R.T., Vale W.W. Presence of growth hormone-releasing factor-like immunoreactivity in rat duodenum. Endocrinology 117:1710, 1985.
Baird A., Wehrenberg W.B., Bohlen P., Ling N. Immunoreactive and biologically active growth hormone-releasing factor in the rat placenta. Endocrinology 117:1598, 1985.
Shibasaki T., Kiyosawa Y., Masuda A., Nakahara M., Imaki T., Wakabayashi I., Demura Shizume K., Ling N. Distribution of growth hormone-releasing hormonelike immunoreactivity in human tissue extracts. J. Clin. Endocrinol. Metab. 59: 263, 1984.
Berry S.A., Pescovitz O.H. Identification of a rat GHRH-like substance and its messenger RNA in rat testis. Endocrinology 123: 661, 1988.
Vannelli B.G., Natali A., Barni T., Serio M., Orlando C., Balboni G.C. Insulin-like growth factor-l (IGF-I) and IGF-I receptor in human testis: an immunohistochemical study. Fertil. Steril. 49:666, 1988.
Fraioli F., Fabbri A., Gnessi L., Silvestroni L., Moretti C., Redi F., Isidori A. Beta-endorphin, met-enkephalin and calcitonin in human semen: evidence for a possible role in human sperm motility. Ann. NY Acad. Sci. 438: 364, 1983.
Schulze W., Davidoff M.S., Holstein A.F. Are Leydig cells of neural origin? Substance P-like immunoreactivity in human testicular tissue. Acta Endocrinol. (Copenh) 115: 337, 1987.
Gnessi L., Isidori A., Bolotti M., Altamura S., Ulisse S., Jannini E.A., Fabbri A., Spera G. Identification of immunoreactive gastrin-releasing peptide related substances in adult Leydig cells. Endocrinology 124: 558, 1989.
Baxter R.C., Martin J.L., Handelsman D.J. Identification of human semen insulin-like growth factor l/somatomedin-C immunoreactivity and binding protein. Acta Endocrinol. (Copenh) 106: 420, 1984.
Spera G., Di Benedetto E., Falaschi P., Fabbri A., Lauro G., Bolotti M. Immunohistochemical localization of Beta-endorphin in hyperplastic interstitial tissue of the human testis. J. Androl. 9:78, 1988.
Hsueh A.J.W., Adashi E.Y., Jones P.B.C., Welsh T.H. jr. Hormonal regulation of the differentiation of cultured ovarian granulosa cells. Endocr. Rev. 5:76, 1984.
Moretti C., Fabbri A., Gnessi L., Fomi L., Fraioli F., Frajese G. GHRH stimulates follicular growth and amplifies FSH-induced ovarian folliculogenesis in women with anovulatory infertility. Reproductive Medicine: Medical Therapy In: G. Frajese, E. Steinberg, LJ. Rodriguez-Rigau (Eds.), Proceedings of the Second International Symposium on Reproductive Medicine, Fiuggi, Italy. Elsevier Science Publishers 875:103, 1989.
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Moretti, C., Fabbri, A., Gnessi, L. et al. Immunohistochemical localization of growth hormone-releasing hormone in human gonads. J Endocrinol Invest 13, 301–305 (1990). https://doi.org/10.1007/BF03349567
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DOI: https://doi.org/10.1007/BF03349567