Journal of Endocrinological Investigation

, Volume 4, Issue 1, pp 75–80 | Cite as

Lipid transport in the hypothyroid rat as reflected by the serum concentrations of free fatty acids, lipoproteins, lecithin-cholesterol acyltransferase and lipoprotein lipase activity in adipose tissue

  • Urban Rosenqvist
  • R. Mahler
  • L. A. Carlson


An experimental model was explored for the study of the effect of thyroid hormone on lipid transport and metabolism in the rat. The development of the hypothyroid state was accompanied by an early decrease in free fatty acids (FFA) followed by an increase in the level of cholesterol and a decrease in triglyceride concentration. Simultaneously, the adipose tissue lipoprotein lipase activity increased while the lecithin-cholesterol acyltransferase was unchanged. The hypothyroid state was also accompanied by a decrease in the concentrations of tryglycerides and cholesterol in very low density lipoproteins (VLDL) while the 2 lipids increased in the low density lipoprotein (LDL) fraction. Administration of triodothyronine markedly reduced the amount of cholesterol in both the LDL and high density lipoprotein (HDL) fractions. The animal model has been shown to have similarities with findings in human subjects.


Triiodothyronine hypothyroidism rat free fatty acids lipoprotein cholesterol triglyceride lecithin-cholesterol acyltransferase lipoprotein lipase 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Malmros H., Swahn B. Lipid metabolism in myxedema. Acta Med. Scand. 145: 361, 1953.PubMedCrossRefGoogle Scholar
  2. 2.
    Nikkilä A., Kekki M. Plasma triglyceride metabolism in thyroid disease. J. Clin. Invest. 51: 2103, 1972.PubMedCentralPubMedCrossRefGoogle Scholar
  3. 3.
    O’Hara D.D., Porte Jr. D., Williams R.H. The effect of diet and thyroxin on plasma lipids in myxoedema. Metabolism 15: 123, 1966.PubMedCrossRefGoogle Scholar
  4. 4.
    Tulloch B.R., Lewis Bl., Russel Fraser T. Triglyceride metabolism in thyroid disease. Lancet 1: 391, 1973.PubMedCrossRefGoogle Scholar
  5. 5.
    Rössner S., Rosenqvist U. Serum lipoproteins and the intravenous fat tolerance test in hypothyroid patients before and during substitution therapy. Atherosclerosis 20: 365, 1974.PubMedCrossRefGoogle Scholar
  6. 6.
    Sandhofer F., Sailer S., Braunsteriner H. Plasmalipide bei Störungen der Schilddrüsenfunktion des Menschen. Klin. Wochenschr. 44: 433, 1966.PubMedCrossRefGoogle Scholar
  7. 7.
    Walton K.W., Scott P.J., Dykes P.W., Davies J.W.L. The significance of alterations in serum lipids in thyroid dysfunction. II. Alterations of the metabolism and turnover of 131I-low density lipoproteins in hypothyroidism and thyrotoxicosis. Clin. Sci. Mol. Med. 29: 217, 1965.Google Scholar
  8. 8.
    Burstein M., Samaille J. Sur un dosage rapid du cholesterol lie aux α-et β-lipo-proteins du serum. Clin. Chirn. Acta 5: 609, 1960.CrossRefGoogle Scholar
  9. 9.
    Carlson K. Lipoprotein fractionation J. Clin. Pathol. (Suppl.) 5: 32, 1973.CrossRefGoogle Scholar
  10. 10.
    Rush R.L., Leon L., Turrell J. Automated simultaneous cholesterol and triglyceride determination on the Auto Analyzer II instrument. Advances in automated analyses Thurman, Miami, 1971, vol. I, p. 503.Google Scholar
  11. 11.
    Kessler G., Lederer H. Fluorimetric measurements of triglycerides. In: Skeggs L.T. (Ed.), Automation in analytical chemistry. Mediad, New York, 1965, vol. 2, p. 341.Google Scholar
  12. 12.
    Noble R.P. Electrophoretic separation of plasma lipoproteins in agarose gel. J. Lipid Res. 9: 693, 1968.PubMedGoogle Scholar
  13. 13.
    Carlson K., Carlson L.A. Comparison of the behaviour of very low density lipoproteins of type III hyperliproteinaemia on electrophoresis on paper and on agarose gel with a note on late (slow) pre-β VLDL lipoprotein. Scand. J. Clin. Lab. Invest. 35: 655, 1975.PubMedCrossRefGoogle Scholar
  14. 14.
    Ho R.J. Radiochemical assay of long-chain fatty acids using 63Ni as tracer. Anal. Biochem. 36: 105, 1970.PubMedCrossRefGoogle Scholar
  15. 15.
    Stokke K.T., Norum K.R. Determination of lecithin: cholesterol acyltransfer in human blood plasma. Scand. J. Clin. Lab. Invest. 27: 21, 1971.PubMedCrossRefGoogle Scholar
  16. 16.
    Nilsson-Ehle P., Törnqvist H., Beifrage P. Rapid determination of lipoprotein lipase activity in human adipose tissue. Clin. Chim. Acta 42: 383, 1972.CrossRefGoogle Scholar
  17. 17.
    Heaf D.J., Kaijser L., Eklund B., Carlson L.A. Differences in heparin-released lipolytic activity in the superficial and deep veins of the human forearm. Eur. J. Clin. Invest. 7: 193, 1977.CrossRefGoogle Scholar
  18. 18.
    Asano Y., Liberman U.A., Edelman I.S. Relationship between Na+-dependent respiration and Na+ + K+-denosine triphosphatase activity in the rat skeletal muscle. J. Clin. Invest. 57: 368, 1976.PubMedCentralPubMedCrossRefGoogle Scholar
  19. 19.
    Rosenqvist U. Effect of thyroidectomy on intracellular amount and distribution of exchanglable Ca++ in isolated rat liver cells. Mol. Cell. Endocrinol. 12: 1978.Google Scholar
  20. 20.
    Shafrir E., Biale Y. Lipoprotein lipase activity in some rat tissues as influenced by various metabolic situations and by nicotine acid. In: Gey K.F., Carlson L.A. (Eds.), Metabolic effects of nicotine acid and its derivatives. Hans Huber, Bern, 1971, p. 515.Google Scholar
  21. 21.
    Jackson R.L, Morrisett J.D., Gotto A.M. Lipoprotein structure and metabolism. Physiol. Rev. 56: 259, 1976.PubMedGoogle Scholar
  22. 22.
    Davies P., Cryer A., Robinson D.S. Hormonal control of adipose tissue clearing factor lipase activity. FEBS Lett. 45: 271, 1974.PubMedCrossRefGoogle Scholar
  23. 23.
    Pykälistö O., Goldberg A.P., Brunzell J.D. Reversal of decreased human adipose tissue lipoprotein lipase and hypertriglyceridemia after treatment of hypo-thyroidism. J. Clin. Endocrinol. Metab. 43: 591, 1976.PubMedCrossRefGoogle Scholar
  24. 24.
    Adlkofer F., Armbrecht U., Schleusener H. Lecithin-cholesterol-acetyltransferase (LCAT) activity in hyper- and hypothyroidism. Acta Endocrinol. (Kbh.) (Suppl.) 173: 143, 1973.Google Scholar

Copyright information

© Italian Society of Endocrinology (SIE) 1981

Authors and Affiliations

  • Urban Rosenqvist
    • 1
  • R. Mahler
    • 1
  • L. A. Carlson
    • 1
  1. 1.King Gustaf V Research Institute and the Department of MedicineKarolinska HospitalStockholm 60Sweden

Personalised recommendations