Journal of Endocrinological Investigation

, Volume 3, Issue 4, pp 365–370 | Cite as

Regulation of 86Rb+ outflow from pancreatic islets III. Possible significance of ATP

  • A. R. Carpinelli
  • W. J. Malaisse


Antimycin A, oligomycin or anoxia augmented 86Rb fractional outflow rate (FOR) from prelabelled pancreatic islets, whether in the absence or presence of glucose. This effect could be due to activation of a Ca2+-sensitive modality of K+ extrusion, being little affected by tetraethylammonium and almost completely suppressed by quinine. In the presence of the latter drug, antimycin A still provoked a modest increase in 86Rb FOR, the magnitude of which was positively related to the extracellular glucose concentration and which could correspond to a discharge of mitochondrial 86Rb. It is proposed that the decrease in 86Rb FOR evoked by insulinotropic nutrients (e.g. glucose) may depend, to a limited extent, on an increase in ATP generation rate.


Isolated islets 86Rb+ ATP antimycin A oligomycin anoxia tetraethylammonium quinine glucose 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Malaisse W.J., Herchuelz A., Devis G., Somers G., Boschero A.C., Hutton J.C., Kawazu S., Sener A., Atwater I.J., Duncan G., Ribalet B., Rojas E. Regulation of calcium fluxes and their regulatory roles in pancreatic islets. Ann. NY Acad. Sci. 307: 562, 1978.PubMedCrossRefGoogle Scholar
  2. 2.
    Sehlin J., Täljedal I.-B. Glucose-induced decrease in Rb+ permeability in pancreatic β-cell. Nature 253: 634, 1975.CrossRefGoogle Scholar
  3. 3.
    Boschero A.C., Kawazu S., Duncan G., Malaisse W.J. Effect of glucose on K+ handling by pancreatic islets. FEBS Lett. 83: 151, 1977.Google Scholar
  4. 4.
    Malaisse W.J., Boschero A.C., Kawazu S., Hutton J.C. The stimulus-secretion coupling of glucose-induced insulin release. XXVII. Effect of glucose on K+ fluxes in isolated islets. Pflügers Arch. 373: 237, 1978.PubMedCrossRefGoogle Scholar
  5. 5.
    Henquin J.C. D-glucose inhibits potassium efflux from pancreatic islets. Nature 271: 271, 1978.PubMedCrossRefGoogle Scholar
  6. 6.
    Boschero A.C., Malaisse W.J. Stimulus-secretion coupling of glucose-induced insulin release. XXIX. Regulation of 86Rb+ efflux from perifused islets. Am. J. Physiol. 236: E139, 1979.PubMedGoogle Scholar
  7. 7.
    Henquin J.C. Opposite effects of intracellular Ca2+ and glucose on K+ permeability of pancreatic islet cells. Nature 280: 66, 1979.PubMedCrossRefGoogle Scholar
  8. 8.
    Henquin J.C. The potassium permeability of pancreatic islet cells: mechanisms of control and influence on insulin release. In: Malaisse W.J., Tälgedal I.-B. (Eds.), Biochemistry and biophysics of the pancreatic β-cell. Horm. Metab. Res. (Supp. 10) Georg Thieme Verlag, Stuttgart, 1980, p. 66.Google Scholar
  9. 9.
    Malaisse W.J., Hutton J.C, Kawazu S., Herchuelz A., Valverde I., Sener A. The stimulus-secretion coupling of glucose-induced insulin release. XXXV. The links between metabolic and cationic events. Diabetologia 16: 331, 1979.PubMedCrossRefGoogle Scholar
  10. 10.
    Carpinelli A.R., Malaisse W.J. Regulation of 86Rb outflow from pancreatic islets. II. Effect of changes in extracellular and intracellular pH. Diabete. Metab. 6: 193, 1980.PubMedGoogle Scholar
  11. 11.
    Hutton J.C, Sener A., Herchuelz A., Valverde I., Boschero A.C, Malaisse W.J. The stimulus-secretion coupling of glucose-induced insulin release. XLII. Effects of extracellular pH on insulin release: their dependency on nutrient concentration. Horm. Metab. Res. 12: 294, 1980.PubMedCrossRefGoogle Scholar
  12. 12.
    Carpinelli A.R., Malaisse W.J. Regulation of 86Rb+ outflow from pancreatic islets. I. Reciprocal changes in the response to glucose, tetraethyl-ammonium and quinine. Mol. Cell. Endocrinol. 17: 103, 1980.PubMedCrossRefGoogle Scholar
  13. 13.
    Ashcroft S.J.H., Weerasinghe L.C.C, Randle P.J. Interrelationship of islet metabolism, adenosine triphosphate content and insulin release. Biochem. J. 132: 223, 1973.PubMedCentralPubMedGoogle Scholar
  14. 14.
    Malaisse W.J:, Sener A., Levy J., Herchuelz A. The stimulus-secretion coupling of glucose-induced insulin release. XXII. Qualitative and quantitative aspects of glycolysis in isolated islets. Acta Diabetol. Lat. 13: 202, 1976.PubMedCrossRefGoogle Scholar
  15. 15.
    Atwater I., Dawson C.M., Ribalet B., Rojas E. Potassium permeability activated by intracellular calcium ion concentration in the pancreatic -cell. J. Physiol. (London) 288: 575, 1979.Google Scholar
  16. 16.
    Hellman B. Calcium and pancreatic β-cell function. 5. Mobilisation of a glucose-stimulated pool of intracellular 45Ca by metabolic inhibitors and the ionophore A-23187. Acta Endocrinol. (Kbh.) 90: 624, 1979.Google Scholar
  17. 17.
    Hille B. lonic channels in nerve membranes. Progr. Biophys. Mol. Biol. 21: 3, 1970.Google Scholar
  18. 18.
    Armando-Hardy M., Ellory J.C., Ferreira M.G., Fleminger S., Lew V.L Inhibition of the calcium-induced increase in potassium permeability of human red cells by quinine. J., Physiol. (London) 250: 32P, 1975.Google Scholar
  19. 19.
    Ravazzola M., Malaisse-Lagae F., Amherdt M., Perrelet A., Malaisse W.J., Orci L. Patterns of calcium localization in pancreatic endocrine cells. J. Cell Sci. 27: 107, 1976.Google Scholar
  20. 20.
    Malaisse W.J., Hutton J.C., Sener A., Levy J., Herchuelz A., Devis G., Somers G. Calcium antagonists and islet function. VII. Effect of calcium deprivation. J. Membr. Biol. 38: 193, 1978.PubMedCrossRefGoogle Scholar
  21. 21.
    Diwan J.J., Tedeschi H. K+ fluxes and the mitochondrial membrane potential. FEBS Lett. 60: 176, 1975.PubMedCrossRefGoogle Scholar
  22. 22.
    Malaisse W.J., Sener A., Boschero A.C., Kawazu S., Devis G., Somers G. The stimulus-secretion coupling of glucose-induced insulin release. XXX. Cationic and secretory effects of menadione in the endocrine pancreas. Eur. J. Biochem. 87: 111, 1978.PubMedCrossRefGoogle Scholar
  23. 23.
    Hellerström C. Effects of carbohydrates on oxygen consumption of isolated pancreatic islets of mice. Endocrinology 81: 105. 1967.PubMedCrossRefGoogle Scholar
  24. 24.
    Hutton J.C., Malaisse W.J. Dynamics of O2 consumption in rat pancreatic islets. Diabetologia, 18: 395, 1980.PubMedGoogle Scholar
  25. 25.
    Carpinelli A.R., Sener A., Herchuelz A., Malaisse W.J. The stimulus-secretion coupling of glucose-induced insulin release. XLI. Effect of intracellular acidification upon calcium efflux from islet cells. Metabolism 29: 540, 1980.PubMedCrossRefGoogle Scholar

Copyright information

© Italian Society of Endocrinology (SIE) 1980

Authors and Affiliations

  • A. R. Carpinelli
    • 1
  • W. J. Malaisse
    • 1
  1. 1.Laboratory of Experimental MedicineBrussels UniversityBrusselsBelgium

Personalised recommendations