Abstract
Leukocyte adherence inhibition (LAI) may be a test for cell-mediated immunity or cy-tophilic antibodies. LAI tests were carried out on patients with Graves’ ophthalmopathy, using normal human lacrimal gland, orbital fat, and eye muscle extracts as antigens. The results were expressed as % LAI. The percentage leukocyte adherence inhibition in tests from patients and normal subjects were not significantly different for any preparation (Mann-Whitney tests). Taking the upper limit of normal as the mean % LAI +2SD for normal subjects, tests were positive, to eye muscle extract, in only one patient whilst borderline inhibition, in response to eye muscle and fat extracts respectively, was demonstrated in two patients. All three had active disease. LAI tests may be positive only during the acute stage of Graves’ ophthalmopathy. The significance of these findings in respect to the pathogenesis of Graves’ ophthalmopathy is discussed.
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Doniach D., Florin-Christensen A. Autoimmunity in the pathogenesis of endocrine exophthalmos. Clin. Endocrinol. Metab. 4: 341, 1975.
Volpé R. The role of autoimmunity in hypoendocrine and hyperen-docrine function: with special emphasis on autoimmune thyroid disease. Ann. Intern. Med. 87: 86, 1977.
Wall J.R., Strakosch C.R., Fang S.L., Joyner D., Ingbar S.H., Braverman L.E. Thyroid binding antibodies and other immunological abnormalities in patients with Graves’ ophthalmopathy: effect of treatment with cyclophosphamide. Clin. Endocrinol. (Oxf.) 10: 79, 1979.
Mahieu P., Winand R. Demonstration of delayed hypersensitivity to retrobulbar and thyroid tissues in human exophthalmos. J. Clin. Endocrinol. Metab. 34: 1090, 1972.
Munro R.E., Lamki L, Row V.V., Volpé R. Cell-mediated immunity in the exophthalmos of Graves’ disease as demonstrated by the migration inhibition fac tor (MIF) test. J. Clin. Endocrinol. Metab. 37: 286, 1973.
Winand R., Mahieu P. Prevention of malignant exophthalmos after treatment of thyrotoxicosis. Lancet 1: 1196, 1973.
Mullin B.R.. Levmson R.E., Friedman A., Henson D.E., Winand R.J., Kohn L.D. Delayed hypersensitivity in Graves’ disease and exophthalmos: identification of thyroglobulin in normal human orbital muscle. Endocrinology 100: 351, 1977.
Wall J.R., Odgers R., Hetzel B.S. Immunological studies of the eye changes of thyrotoxicosis. Aust. N.Z.J. Med. 3: 162, 1973.
Day R. Clinical and pathological manifestations of eye changes, the thyroid: a functional and clinical test. In: Werner S.C., Ingbar S.H. (Eds.), The thyroid. Harper & Row, New York. 1971. p. 539.
Halliday W.J., Miller S. Leukocyte adherence inhibition: a simple test for cell-mediated tumor immunity and serum blocking factors. Int. J. Cancer. 9: 477, 1972.
Halliday W.J., Maluish A.E., Isbister W.H. Detection of anti-tumor cell-mediated immunity and serum blocking factors in cancer patients by the leukocyte adherence inhibition test. Br. J. Cancer 29: 31, 1974.
Grosser N., Marti J.H., Proctor J.W., Thomson D.M.P. Tube leukocyte adherence inhibition assay for the detection of anti-tumor immunity. I. Monocyte is the reactive cell. Int. J. Cancer 18: 39, 1976.
Marti J.H., Grosser N., Thomson D.M.P. Tube leukocyte adherence inhibition assay for the detection of anti-tumor immunity II. Monocyte reacts with tumor antigen via cytophilic anti-tumor antibody. Int. J. Cancer 18: 48, 1976.
Boyum A. Separation of leukocytes from blood and bone marrow. Scand. J. Clin. Lab. Invest. 21: 9, 1968. (Suppl. 97).
Holan V., Hasek M., Pubenik J., Chutna J. Antigen-mediated macrophage adherence inhibition. Cell, immunol. 13: 107, 1974.
Grosser N., Thomson D.M.P. Cell-mediated anti-tumor immunity in breast cancer patients evaluated by antigen induced leukocyte adherence inhibition in test tubes. Cancer Res. 35: 2571, 1975.
Walters B.A., Chick J.E., Halliday W.J. Cell-mediated immunity and serum blocking factors in patients with chronic dermatophytic infections. Int. Arch. Allergy Appl. Immunol. 46: 49, 1974.
Rutherford J.C., Walters B.A.J., Cavaye G., Halliday W.J. A modified leukocyte adherence inhibition test in the laboratory investigation of gastrointestinal cancer. Int. J. Cancer 19: 43, 1977.
Schnieder W.C., Hogeboom G.H. Intracellular distribution of enzymes: V. Further studies on the distribution of cytochrome c in rat liver homogenates. J. Biol. Chem. 183: 123, 1950.
Wall J.R., Walters B.A. Peripheral blood lymphocyte transformation and leukocyte adherence inhibition in response to human testis fractions in patients with lepromatous leprosy. (Submitted).
Teng C.S., Smith B.R., Clayton B., Evered D.C., Clark F., Hall R. Thyroid-stimulating immunoglobulins in ophthalmic Graves’ disease. Clin. Endocrinol. (Oxf.) 6: 207, 1977.
Solomon D.H., Chopra I.J., Chopra U., Smith F.J. Long-acting thyroid stimulator protector assay as a basis for identification of subgroups of Graves’ ophthalmopathy. Trans. Assoc. Phys. 89: 159, 1976.
Wall J.R., Trewin A., Fang S.L., Ingbar S.A., Braverman L.E. Studies of immunoreactivity to human lacrimal gland fractions in patients with ophthalmic Graves’ disease. J. Endocrinol. Invest. 1: 253, 1978.
Etienne J., Kohn L.D., Winand R.J. Studies on the mechanisms of experimental exophthalmos. In: Robbins J., Braverman L.E. (Eds.), Thyroid Research. Excerpta Medica International Congress Series 378, Amsterdam, 1976, p. 380.
Fakhri O., Hobbs J., R. Detection of antibodies which can cooperate with lymphocytes. Lancet 2: 403, 1972.
Kohn L.D., Winand R.J. Relationship of thyrotropin to exophthalmos-producing substance. J. Biol. Chem. 246: 6570, 1971.
Konishi J., Herman M.M., Kriss J.P. Binding of thyroglobulin-antithyroglobulin immune complex to extraocular muscle membrane. Endocrinology 95: 434, 1974.
Kriss J.P., Medhi S.Z., Sheng R. Mechanisms of cell-mediated cytotoxicity in Graves’ ophthalmopathy. Proceedings of 54th Meeting of American Thyroid Association, Portland, Oregon, September 13–16, 1978. Endocrinology 103 (Suppl.): T1 1978 (Abstract).
Wall J.R., Joyner D.M., Ryan E.A., Wren S.G.F. Effect of experimental hyper and hypothyroidism on B and T lymphocyte reactivity to bacterial, fungal and tissue antigens. In: Pinchera A., Doniach D., Fenzi G.F., Baschieri L. (Eds.), Symposium on Autoimmune Aspects of Endocrine Disorders. Academic Press, New York, in press.
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Wall, J.R., Walters, B.A.J. & Grant, C. Luekocyte adherence inhibition in response to human orbital and lacrimal extracts in patients with Graves’ ophthalmopathy. J Endocrinol Invest 2, 375–378 (1979). https://doi.org/10.1007/BF03349336
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DOI: https://doi.org/10.1007/BF03349336