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Journal of Endocrinological Investigation

, Volume 8, Issue 5, pp 455–458 | Cite as

The influence of gonadotropic hormones on the EGF receptor regulation in the rat ovary

  • J. W. Siebers
  • T. Bauknecht
  • A. Schopfer
  • M. Breckwoldt
Article

Abstract

Ovaries of immature rats are endowed with only a small number of EGF binding sites (59.2 × 10−15 mol/mg protein). During development from day 20 to 28, the binding capacity increases 2.5 times, while the growth of the ovaries remains small. Treatment with FSH on day 20 advances the EGF binding capacity with a 4-fold increase in the number of binding sites within the first two days. HCG has no or only a slight effect on the EGF binding capacity. The EGF receptor formation after FSH treatment takes place before the ovaries begin to grow rapidly. Accelerated ovarian growth is associated with a decrease of EGF binding capacity reaching values below those of the control animals during the subsequent days. The dissociation constants (KD) of the FSH stimulated receptors and of receptors present during development are found in the order of 2.7 and 8.7 × 10−9 M. The physiological function of the EGF receptor for ovarian cell proliferation and follicular growth is discussed.

Key-words

Ovary EGF receptor development gonadotropic hormones receptor regulation 

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References

  1. 1.
    Pedersen T. Follicle growth in the immature mouse ovary. Acta Endocrinol. (Kbh.) 62: 117, 1969.Google Scholar
  2. 2.
    Peters H., Byskov A.G., Himelstein-Braw R., Faber M. Follicular growth: the basic event in the mouse and human ovary. J. Reprod. Fertil. 45: 559, 1975.PubMedCrossRefGoogle Scholar
  3. 3.
    Eshkol A., Lunenfeld B. Gonadotropic regulation of ovarian development in mice during infancy. In: Saxena B.B., Beling C.G., Gandy H.M. (Eds.), Gonadotropins. John Wiley & Sons, New York, 1972, p. 335.Google Scholar
  4. 4.
    Goldenberg R.L., Reiter F.O., Ross G.T. Follicle response to exogenous gonadotropins: an estrogen-mediated phenomen. Fertil. Steril. 24: 121, 1973.PubMedGoogle Scholar
  5. 5.
    Richards J.S., Midgley A.R. Jr. Protein hormone action: a key to understanding ovarian follicular and luteal cell development. Biol. Reprod. 14: 82, 1976.PubMedCrossRefGoogle Scholar
  6. 6.
    Makris A., Klagsbrun M.; Yasumizu T., Ryan K.J. An endogenous ovarian growth factor which stimulates BALB/3T3 and granulosa cell proliferation. Biol. Reprod. 29: 1135, 1983.PubMedCrossRefGoogle Scholar
  7. 7.
    Vlodavsky I., Brown K.D., Gospodarowicz D. A comparison of the binding of epidermal growth factor to cultured granulosa and luteal cells. J. Biol. Chem. 253: 3744, 1978.PubMedGoogle Scholar
  8. 8.
    Gospodarowicz D., Ill C.R., Birdwell C.R. Effects of fibroblast and epidermal growth factor on ovarian cell proliferation in vitro. I. Characterization of the response of granulosa cells to FGF and EGF. Endocrinology 100: 1108, 1977.PubMedCrossRefGoogle Scholar
  9. 9.
    Gospodarowicz D., Bialecki H. Fibroblast and epidermal growth factors are mitogenic agents for cultured granulosa cells of rodent, porcine and human origin. Endocrinology 104: 757, 1979.PubMedCrossRefGoogle Scholar
  10. 10.
    Bauknecht T., Rau B., Meerpohl H.G., Pfleiderer A. The prognostic value of the presence of EGF receptors in the ovarian carcinoma. Tumor Diagnostic Therapeutics 5: 62, 1984.Google Scholar
  11. 11.
    Carpenter G., Cohen S. 125I-labeled human epidermal growth factor. Binding, internalization and degradation in human fibroblasts. J. Cell Biol. 77: 159, 1976.CrossRefGoogle Scholar
  12. 12.
    Scatchard G. The attraction of protein for small molecules and ions. Ann N.Y. Acad. Sci. 51: 660, 1949.CrossRefGoogle Scholar
  13. 13.
    Jones P.B.C., Welsh T.H., Hsueh A.J.W. Regulation of ovarian progestin production by epidermal growth factor in cultured rat granulosa cells. J. Biol. Chem. 257: 11268, 1982.PubMedGoogle Scholar
  14. 14.
    Cohen S. The stimulation of epidermal proliferation by a specific protein. Dev. Biol. 72: 394, 1965.CrossRefGoogle Scholar
  15. 15.
    Gospodarowicz D., Ill C.R., Birdwell C.R. Effects of fibroblast and epidermal growth factors on ovarian cell proliferation in vitro. II Proliferative response of luteal cells to FGF but not EGF. Endocrinology 100: 1121, 1977.PubMedCrossRefGoogle Scholar
  16. 16.
    Lintern-Moore S., Moore G.P.M., Panaretto B.A., Robertson D. Follicular development in neonatal mouse ovary: effect of epidermal growth factor. Acta Endocrinol. (Kbh.) 96: 123, 1981.Google Scholar
  17. 17.
    Hsueh A.J.W., Welsh. T.H., Jones P.B.C. Inhibition of ovarian and testicular steroidogenesis by epidermal growth factor. Endocrinology 108: 2002, 1981.PubMedCrossRefGoogle Scholar
  18. 18.
    Cohen S., Savage C.R. Jr. Part. II. Recent studies on the chemistry and biology of epidermal growth factor. Recent Prog. Horm. Res. 30: 551, 1974.PubMedGoogle Scholar
  19. 19.
    Carpenter G., Cohen S. Epidermal growth factor. Annu. Rev. Biochem. 48: 193, 1979.PubMedCrossRefGoogle Scholar

Copyright information

© Italian Society of Endocrinology (SIE) 1985

Authors and Affiliations

  • J. W. Siebers
    • 1
  • T. Bauknecht
    • 1
  • A. Schopfer
    • 1
  • M. Breckwoldt
    • 1
  1. 1.Universitäts-FrauenklinikFreiburgGermany

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