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Determination of oxidative protein and lipid damage in adult hypopituitary patients with GH deficiency

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Abstract

The aim of this study is to determine oxidative protein and lipid damage in adult hypopituitary GH-deficient patients. Eighteen hypopituitary GH-deficient — otherwise healthy — adults on conventional replacement therapy other than GH (9 male, 9 female, age 41.8±16.4 yr) and 18 healthy subjects (6 male, 12 female, age 40.3±16.2 yr) participated in the study. Plasma products of oxidative protein damage [protein carbonyl (PCO) and nitrotyrozine (NT)], plasma oxidized LDL (oxLDL), plasma product of oxidative lipid damage [lipid hydroperoxide (LHP)] and antioxidant status of the plasma [total thiol (T-SH)] were measured. Body fat percentage, total and LDL-cholesterol concentrations were significantly higher in the hypopituitary group. Plasma PCO, NT, LHP and T-SH concentrations did not differ significantly between patients and controls. OxLDL concentration was significantly higher in the hypopituitary patients (62.4±17.8 vs 43.1±11.3 U/l, p=0.001). In the patients, oxLDL correlated significantly with the duration of hypopituitarism (r=0.6323, p=0.01). In the controls, oxLDL correlated significantly with blood pressure, total and VLDL-cholesterol concentrations. Increased oxLDL concentration may indicate increased oxidative stress within the vascular compartment and may contribute to the proatherogenic state in GH-deficient hypopituitary patients independent from conventional risk factors.

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Authors and Affiliations

  1. Department of Internal Medicine, Division of Endocrinology, Istanbul University, Çapa, Merter/Istanbul, Turkey

    N. Özbey MD, A. Yurci & S. Molvalilar

  2. Central Laboratory of Clinical Biochemistry, Faculty of Medicine, Istanbul University, Çapa, Istanbul, Turkey

    A. Telci & U. Cakatay

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  1. N. Özbey MD
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  2. A. Telci
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Correspondence to N. Özbey MD.

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Özbey, N., Telci, A., Cakatay, U. et al. Determination of oxidative protein and lipid damage in adult hypopituitary patients with GH deficiency. J Endocrinol Invest 26, 1001–1007 (2003). https://doi.org/10.1007/BF03348199

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