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Journal of Endocrinological Investigation

, Volume 9, Issue 3, pp 209–215 | Cite as

Effects of beta non-selective and beta1 selective adrenergic blocking agents on glucagon secretion from isolated perfused rat pancreas

  • F. Gregorio
  • P. Filipponi
  • S. Cristallini
  • C. Carloni
  • I. Moretti
  • C. Ferrandina
  • R. Pippi
  • M. Pietropaolo
Article

Abstract

To characterize beta-receptors which affect pancreatic A-cell activity, the effects of propranolol (beta non-selective blockade) and metoprolol (beta1 selective blockade) were evaluated on epinephrine modulated insulin (IRI) and glucagon (IRG) release both in basal state and during metabolic stimulus (arginine 20 mM). The isolated perfused rat pancreas model with the exclusion of stomach and duodenum was used. Epinephrine infusion (at 10−7 M) caused a prompt and sustained increase in basal IRG secretion and significantly potentiated glucagon release in response to metabolic stimulus. Insulin secretion was markedly suppressed by epinephrine both in basal conditions and during metabolic stimulus. Propranolol (at 10−7 M) and metoprolol (at 10−7 M) infusion clearly and similarly counteracted epinephrine stimulatory effects on IRG secretion but failed to elicit any significant effect on the epinephrine inhibited IRI release either in basal state or during the metabolic stimulus. These results suggest that, at least in the rat, the adrenergic stimulation of IRG release is mediated through a beta1 receptor.

Key-words

Beta (non) selective blockade adrenergic control glucagon secretion isolated perfused pancreas 

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Copyright information

© Italian Society of Endocrinology (SIE) 1986

Authors and Affiliations

  • F. Gregorio
    • 1
  • P. Filipponi
    • 1
  • S. Cristallini
    • 1
  • C. Carloni
    • 1
  • I. Moretti
    • 1
  • C. Ferrandina
    • 1
  • R. Pippi
    • 2
  • M. Pietropaolo
    • 2
  1. 1.Istituto di Clinica Medica I, Policlinico MontelucePerugiaItaly
  2. 2.Istituto di Patologia Speciale Medica, Policlinico MontelucePerugiaItaly

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