Journal of Endocrinological Investigation

, Volume 14, Issue 10, pp 821–824 | Cite as

Insulin and insulin-like growth factor I exert different effects on plasminogen activator production or cell growth in the ovine thyroid cell line OVNIS

  • B. Degryse
  • F. Maisonobe
  • S. Hovsépian
  • G. Fayet


Insulin and Insulin-like Growth Factor I (IGF-I) are evaluated for their capacity to affect cell proliferation and plasminogen activator (PA) activity production in an ovine thyroid cell line OVNIS. Insulin at physiological and supraphysiological doses induces cell proliferation and increases PA activity. IGF-I, which is also clearly mitogenic for these cells, surprisingly does not modulate PA activity. The results indicate that the growth promoting effect is mediated through the insulin and IGF-I receptors whereas PA activity is solely regulated via the insulin receptors.


Thyroid thyroid cell insulin insulin-like growth factor I plasminogen activator cell growth 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Debant A., Clauser E., Ponzio G., Filloux C., Auzan C., Contreres J.-O., Rossi B. Replacement of insulin receptor tyrosine residues 1162 and 1163 does not alter the mitogenic effect of the hormone. Proc. Natl. Acad. Sci. USA 85: 8032, 1988.PubMedCentralPubMedCrossRefGoogle Scholar
  2. 2.
    Chomczynski P., Qasba P., Topper Y.J. Essential role of insulin in transcription of the rat 25,000 molecular weight casein gene. Science 226: 1326, 1984.PubMedCrossRefGoogle Scholar
  3. 3.
    Sho K., Kondo Y. Insulin modulates thyrotropin-induced follicle reconstruction and iodine metabolism in hog thyroid cells cultured in a chemically defined medium. Biochem. Biophys. Res. Commun. 118: 385, 1984.PubMedCrossRefGoogle Scholar
  4. 4.
    Straus D.S. Growth-stimulatory actions of insulin in vitro and in vivo. Endocr. Rev. 5: 356, 1984.PubMedCrossRefGoogle Scholar
  5. 5.
    Rinderknecht E., Humbel R.E. The amino acid sequence of human insulin-like growth factor I and its structural homology with proinsulin. J. Biol. Chem. 253: 2769, 1978.PubMedGoogle Scholar
  6. 6.
    Zapf J., Schoenle E., Froesch E.R. Insulin-like growth factor I and II: same biological actions and receptor binding characteristics of two purified constituents of non suppressible insulin-like activity of human serum. Eur. J. Biochem. 87: 285, 1978.PubMedCrossRefGoogle Scholar
  7. 7.
    Massagué J., Czech M.P. The subunit structure of two distinct receptors for insulin-like growth factors I and II and their relationship to the insulin receptor. J. Biol. Chem. 257: 5038, 1982.PubMedGoogle Scholar
  8. 8.
    Coscieri M.A., Chicchi G.G., Hayes N.S., Strader C.D. (Thr-59)-insulin like growth factor I stimulates 2-deoxyglucose transport in BC3H1 myocytes through the insulin-like growth factor receptor, not the insulin receptor. Biochim. Biophys. Acta 886: 491, 1986.CrossRefGoogle Scholar
  9. 9.
    Hornsby P.J., Hancock J.P., Vo T.P., Nason L.M., Ryan R.F., McAllister J.M. Loss of expression of a differentiated function gene, steroid 17 α-hydroxylase, as adrenocortical cells senesce in culture. Proc. Natl. Acad. Sci. USA 84: 1580, 1987.PubMedCentralPubMedCrossRefGoogle Scholar
  10. 10.
    Trischitta V., Damante G., Foti D., Filetti S. Insulin binding and biological activities in the FRTL-5 rat thyroid cell line. Metabolism 36: 379, 1987.PubMedCrossRefGoogle Scholar
  11. 11.
    Roger P.P., Dumont J.F. Factors controlling proliferation and differentiation of canine thyroid cells cultured in reduced serum conditions: effects of thyrotropin, cyclic AMP and growth factors. Mol. Cell. Endocrinol. 36: 79, 1984.PubMedCrossRefGoogle Scholar
  12. 12.
    Williams D.W., Wynford-Thomas D., Williams E.D. Control of human thyroid follicular cell proliferation in suspension and monolayer culture. Mol. Cell. Endocrinol. 51: 33, 1987.PubMedCrossRefGoogle Scholar
  13. 13.
    Fayet G., Hovsépian S. Strategy of thyroid cell culture in defined media and the isolation of the OVNIS and PORTHOS cell strains. In: Eggo M.C., Burrow G.N. (Eds.), Progress in Endocrine Research. Raven Press, New York, 1985, vol. 2, p. 211.Google Scholar
  14. 14.
    Aouani A., Hovsépian S., Fayet G. Multihormonal regulation of thyroglobulin production by the OVNIS 6H thyroid cell line. Horm. Metab. Res. 20: 91, 1988.PubMedCrossRefGoogle Scholar
  15. 15.
    Tode B., Serio M., Rotella C.M., Galli G., Franceschelli F., Tanini A., Toccafondi R. Insulin-like growth factor I: autocrine secretion by human thyroid follicular cells in primary culture. J. Clin. Endocrinol. Metab. 69: 639, 1989.PubMedCrossRefGoogle Scholar
  16. 16.
    Bachrach L.K., Eggo M.C., Hintz R.L., Burrow G.N. Insulin-like growth factors in sheep thyroid cells: action, receptors and production. Biochem. Biophys. Res. Commun. 154: 861, 1988.PubMedCrossRefGoogle Scholar
  17. 17.
    Tramontano D., Moses A.C., Picone R., Ingbar S.H. Characterization and regulation of the receptors for insulin-like growth factor I in the FRTL-5 rat thyroid follicular cell line. Endocrinology 120: 785, 1987.PubMedCrossRefGoogle Scholar
  18. 18.
    Saksela O. Plasminogen activation and regulation of pericellular proteolysis. Biochim. Biophys. Acta 823: 35, 1985.PubMedGoogle Scholar
  19. 19.
    Ambesi-Impiombato F.S., Parks L.A.M., Coon H.G. Culture of hormone-dependent functional epithelial cells from rat thyroids. Proc. Natl. Acad. Sci. USA 77: 3455, 1980.PubMedCentralPubMedCrossRefGoogle Scholar
  20. 20.
    Labarca C., Paigen K. A simple, rapid and sensitive DNA assay procedure. Anal. Biochem. 102: 344, 1980.PubMedCrossRefGoogle Scholar
  21. 21.
    Brisson-Lougarre A., Blum C.J. Liaison de l’insuline aux membranes plasmiques isolées thyroidiennes. C.R. Séances. Acad. Sci 293: 275, 1981.Google Scholar
  22. 22.
    Mak W.W.-N., Eggo M.C., Burrow G.N. Thyrotropin regulation of plasminogen activator activity in primary cultures of ovine thyroid cells. Biochem. Biophys. Res. Commun. 123: 633, 1984.PubMedCrossRefGoogle Scholar
  23. 23.
    Rijken D.C., Wijngaards G., Welbergen J. Immunological characterization of plasminogen activators activities in human tissues and body fluids. J. Lab. Clin. Med. 97: 477, 1981.PubMedGoogle Scholar
  24. 24.
    Cassano S., Ragno P., Blasi F., Rossi G. Production of urokinase-type plasminogen activator by normal and transformed rat thyroid cells in culture. Exp. Cell. Res. 182: 197, 1989.PubMedCrossRefGoogle Scholar
  25. 25.
    Aouani A. Caractérisation d’une lignée continue de cellules thyroïdiennes ovines hormonosensibles: OVNIS. Régulation multihormonale de la synthèse de thyroglobuline. Microscopie Moléculaire. Thesis, Université d’Aix-Marseille II, 1987.Google Scholar
  26. 26.
    Rotella C.M., Mavilia C., Frediani U., Toccafondi R. Calf serum modifies the mitogenic activity of epidermal growth factor in WRT thyroid cells. Mol. Cell. Endocrinol. 65: 63, 1989.PubMedCrossRefGoogle Scholar
  27. 27.
    Drews R., Ferguson D.C., Gerber H., Peterson M.E., McGraw R.A., Halper J., Becker D.V. Effects of serum and growth factors on proliferation of cultured normal and adenomatous feline thyrocytes. Ann. Endocrinol. (Paris) 50: 143, 1989 (Abstract).Google Scholar
  28. 28.
    Massague J., Blinderman L.A., Czech M.P. The high affinity insulin receptor mediates growth stimulation in rat hepatoma cells. J. Biol. Chem. 257: 13958, 1982.PubMedGoogle Scholar
  29. 29.
    Takasu N., Takasu M., Komiya I., Nagasawa Y., Asawa T., Shimizu Y., Yamada T. Insulin-like growth factor I stimulates inositol phosphate accumulation, a rise in cytoplasmic free calcium, and proliferation in cultured porcine thyroid cells. J. Biol. Chem. 264: 18485, 1989.PubMedGoogle Scholar
  30. 30.
    Tramontano D., Cushing G.W., Moses A.C., Ingbar S.H. Insulin-like growth factor I stimulates the growth of rat thyroid cells in culture and synergizes the stimulation of DNA synthesis induced by TSH and Graves’ IgG. Endocrinology 119: 940, 1986.PubMedCrossRefGoogle Scholar

Copyright information

© Italian Society of Endocrinology (SIE) 1991

Authors and Affiliations

  • B. Degryse
    • 1
  • F. Maisonobe
    • 1
  • S. Hovsépian
    • 1
  • G. Fayet
    • 1
  1. 1.Faculté de MédecineLaboratoire de Biochimie Médicale et U 38 INSERMMarseille Cédex 5France

Personalised recommendations