Changes of pituitary and penile structure in male adult rats following castration and high-fat diet
- 35 Downloads
Aim: To investigate the influence of low androgen levels and high-fat diet on the structure of pituitary and penis in male rats. Methods: Ten-week-old adult male Sprague-Dawley rats were randomly divided into 2 groups, one fed a high-fat diet the other fed a normal diet; each group consisted of 3 subgroups: controls, castrated rats (with low androgen), and castrated rats given undecanoate replenishment. After 11 weeks, the structure of pituitary and penis were observed under light microcopy. Immunohistochemistry was used to assess the expression of FSH in pituitary and cyclooxygenase-2 (COX-2) in corpora cavernosa penis. Results: The structures of pituitary and penis in castrated rats were injured, and were more damaged in castration together with high-fat diet. Immunohistochemistry showed FSH expression in castrated rats pituitary while castrated rats on a high-fat diet had less positive staining than those on a normal diet. Vascular structure of corpora cavernosa penis, showed a strongly positive COX-2 expression in high-fat diet rats. Conclusions: Castration and high-fat diet could induce structural damages of pituitary and penis in male rats. Replacement with testosterone could partially restore the impaired structure. The positive expression of COX-2 implied inflammatory pathway existence on vascular structure of penis in high-fat diet and low-androgen male rats.
Key-wordsCastration high-fat penile pituitary
Unable to display preview. Download preview PDF.
- 10.Ponglowhapan S, Church DB, Khalid M. Expression of cyclooxygenase-2 in the canine lower urinary tract with regard to the effects of gonadal status and gender. Theriogenology 2009, 71: 1276–88.Google Scholar
- 17.Hayes FJ, DeCruz S, Seminara SB, Boepple PA, Crowley WF Jr. Differential regulation of gonadotropin secretion by testosterone in the human male: absence of a negative feedback effect of testosterone on follicle-stimulating hormone secretion. J Clin Endocrinol Metab 2001, 86: 53–8.PubMedGoogle Scholar