Journal of Endocrinological Investigation

, Volume 16, Issue 1, pp 21–27 | Cite as

Molecular composition of two different batches of urofollitropin: Analysis by immunofluorimetric assay, radioligand receptor assay and in vitro bioassay

  • M. Simoni
  • G. F. Weinbauer
  • E. Nieschlag


The molecular heterogeneity of two different batches of commercially available urofollitropin was analyzed after fractionation by isoelectric focusing (IEF). FSH was measured before and after IEF by a highly specific time-resolved immunofluorimetric assay (IFMA), by a radioligand receptor assay (RRA) employing a preparation of calf testis FSH receptors, and by the in vitro bioassay based on FSH-dependent aromatase stimulation in immature rat Sertoli cells. An overall good correspondence between the results obtained with the three different methods was observed. However, the RRA and the in vitro bioassay appeared to be more suitable than the IFMA in resolving individual FSH isoforms. The mean isoelectric points of the two FSH preparations analyzed were slightly different, due to different molecular composition. These differences, however, seem too minute to be considered as cause of the different pharmacokinetics of FSH described in the literature or to explain the inconsistent therapeutical results seen in patients treated with FSH of urinary origin.

Key words

FSH in vitro bioassay RRA IFMA isoforms of FSH 


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Copyright information

© Italian Society of Endocrinology (SIE) 1993

Authors and Affiliations

  • M. Simoni
    • 1
  • G. F. Weinbauer
    • 1
  • E. Nieschlag
    • 1
  1. 1.Institute of Reproductive Medicine of the University and WHO Collaborating Center for Research in Human ReproductionMünsterGermany

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