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Journal of Endocrinological Investigation

, Volume 29, Issue 4, pp 342–349 | Cite as

One-tube-PCR technique for CCL2, CCL3, CCL4 and CCL5 applied to fine needle aspiration biopsies shows different profiles in autoimmune and non-autoimmune thyroid disorders

  • X. Ferrer-Francesch
  • P. Caro
  • L. Alcalde
  • M. P. Armengol
  • Y. Ashhab
  • A. Lucas-Martín
  • E. M. Martínez-Cáceres
  • M. Juan
  • R. Pujol-Borrell
Original Articles

Abstract

Autoimmune thyroid diseases are characterized by lymphocytic infiltration of the thyroid gland. Chemokines are crucial in the recruitment of lymphocytes and might play an important role in the pathogenesis of autoimmune thyroid disease. The aim of this study was to test the feasibility of analysing by one-tube reverse-transcriptase polymerase chain reaction (RT-PCR) technique CC chemokine profiles in samples obtained by fine needle aspiration biopsy (FNAB). In 27 out of 35 (77%) samples, the material was sufficient for analysis and in 16 (59%) chemokines were detected, thus demonstrating the potential of this technique. Moreover, even in this small group, a statistically significant increase of CCL3 and CCL4 was found in samples from patients with autoimmune thyroid disease as compared to those with multinodular goiter. Chemokine profile measured by improved multiamplification techniques in FNAB thyroid samples may become a useful complementary tool for the management of thyroid autoimmune disease as it constitutes a source of data for research of their pathogenesis.

Key Words

Autoimmune thyroid disease PCR chemokine FNAB CCL2 CCL3 CCL4 CCL5 

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Copyright information

© Italian Society of Endocrinology (SIE) 2006

Authors and Affiliations

  • X. Ferrer-Francesch
    • 1
    • 3
  • P. Caro
    • 1
  • L. Alcalde
    • 1
  • M. P. Armengol
    • 1
    • 3
  • Y. Ashhab
    • 1
  • A. Lucas-Martín
    • 2
  • E. M. Martínez-Cáceres
    • 1
  • M. Juan
    • 1
    • 3
  • R. Pujol-Borrell
    • 1
    • 3
  1. 1.Immunology (Laboratory of Immunology for Research Applied to Diagnosis (LIRAD)Blood and Tissue BankSpain
  2. 2.Endocrinology DivisionInstitute for Health Sciences Research Germans Trias i PujolBadalona
  3. 3.Division of Immunology, Dept of Cell Biology, Physiology and Immunology, Faculty of MedicineAutonomous University of BarcelonaBellaterraSpain

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