Journal of Endocrinological Investigation

, Volume 23, Issue 9, pp 609–615 | Cite as

Role of c-kit in mammalian spermatogenesis

  • P. Rossi
  • C. Sette
  • S. Dolci
  • R. Geremia


The tyrosine-kinase receptor c-kit and its ligand, stem cell factor (SCF), are essential for the maintenance of primordial germ cells (PGCs) in both sexes. However, c-kit and a postmeiotic- specific alternative c-kit gene product play important roles also during post-natal stages of spermatogenesis. In the adult testis, the c-kit receptor is re-expressed in differentiating spermatogonia, but not in spermatogonial stem cells, whereas SCF is expressed by Sertoli cells under FSH stimulation. SCF stimulates DNA synthesis in type A spermatogonia cultured in vitro, and injection of anti-c-kit antibodies blocks their proliferation in vivo. A point mutation in the c-kit gene, which impairs SCF-mediated activation of phosphatydilinositol 3-kinase, does not cause any significant reduction in PGCs number during embryonic development, nor in spermatogonial stem cell populations. However males are completely sterile due to a block in the initial stages of spermatogenesis, associated to abolishment of DNA-synthesis in differentiating A1-A4 spermatogonia. With the onset of meiosis c-kit expression ceases, but a truncated c-kit product, tr-kit, is specifically expressed in post-meiotic stages of spermatogenesis, and is accumulated in mature spermatozoa. Microinjection of tr-kit into mouse eggs causes their parthenogenetic activation, suggesting that it might play a role in the final function of the gametes, fertilization.


C-kit stem cell factor spermatogenesis fertilization 


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Copyright information

© Italian Society of Endocrinology (SIE) 2000

Authors and Affiliations

  1. 1.Dipartimento di Sanità Pubblica e Biologia Cellulare, Sezione di AnatomiaUniversità “Tor Vergata”RomaItaly

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