Journal of Endocrinological Investigation

, Volume 23, Issue 4, pp 218–222 | Cite as

Morphology of FRTL-5 cell colonies in a semi-solid medium

  • Z. Pajer
  • A. Cör
  • D. Stiblar-Martincic
Original Article


For the first time, 3, 7 and 10 days growth of normal thyroid follicular FRTL-5 cell colonies in a semi-solid medium of 0.6% methocel over 1% agar base was morphometrically analyzed. It was found that the areas of FRTL-5 colonies as well as their perimeters and maximum diameters increased, while according to their form factors the FRTL-5 colonies were regular in shape. After 10 days in a semi-solid medium, 83% of the FRTL-5 colonies had maximum diameters greater than 50 gMm. It is obvious that after long culturing of FRTL-5 cells under the influence of the pituitary thyroid-stimulating hormone (TSH) these cells are not uniform anymore and that they grow in a semi-solid medium.


FRTL-5 cell colonies methocel morphometry 


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Copyright information

© Italian Society of Endocrinology (SIE) 2000

Authors and Affiliations

  1. 1.Institute of Histology and Embryology, Medical FacultyUniversity of LjubljanaLjubljanaSlovenia

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