Journal of Endocrinological Investigation

, Volume 23, Issue 1, pp 1–4 | Cite as

In vitro expression of proα1(I) collagen mRNA by human pre-osteoclastic cells

  • L. Masi
  • C. Malentacchi
  • S. Benvenuti
  • A. Amedei
  • U. Bigozzi
  • E. Montali
  • Maria Luisa Brandi
Original Article


In vitro studies have demonstrated that the extracellular matrix modulates the cell phenotype. In the present study we have investigated in vitro proα1(I) collagen mRNA expression in a human pre-osteoclastic cell line (FLG 29.1 cells) in basal condition and after various stimuli. In addition, in order to evaluate the effect of cell-cell interactions on collagen type I mRNA expression, we have cultured the human pre-osteoclastic cells FLG 29.1 with either the human osteoblast-like cell line Saos-2 or the bovine bone endothelial cell line BBE. We showed that the FLG 29.1 cells express proα1(I) collagen mRNA, whose expression is modulated by phorbol esters (TPA). Co-culturing FLG 29.1 cells with either Saos-2 or BBE cells induced decrease of proα1(I) collagen mRNA expression.


Collagen type I preosteoclastic cells bone endothelial cells osteoblastic cells 


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Copyright information

© Italian Society of Endocrinology (SIE) 2000

Authors and Affiliations

  • L. Masi
    • 1
  • C. Malentacchi
    • 2
  • S. Benvenuti
    • 1
  • A. Amedei
    • 1
  • U. Bigozzi
    • 2
  • E. Montali
    • 1
  • Maria Luisa Brandi
    • 2
  1. 1.Endocrine Unit, Department of Clinical PhysiopathologyUniversity of FirenzeFlorenceItaly
  2. 2.Genetic Unit, Department of Clinical PhysiopathologyUniversity of FirenzeItaly

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