Journal of Thermal Analysis and Calorimetry

, Volume 51, Issue 3, pp 747–755 | Cite as

Heat-Induced Gelation of Globular Protein Mixtures

A DSC and scanning electron microscopic study
  • P. Relkin
  • T. Meylheuc
  • B. Launay
  • K. Raynal
JCAT′97 28th Conference on Calorimetry and Thermal Analysis Dunkirk, France May 27–30, 1997


Heat-induced conformational changes and heat-induced gels of whey and egg white albumen, and their major components were studied under physicochemical conditions that favour protein-protein interactions. We used differential scanning calorimetry (DSC) to compare their conformational heat stability, through the characteristic temperature (Θmax) corresponding to the maximal heat flow and the overall calorimetric heat of reaction (Δr H cal). Times needed to observe sol-gel state transitions at various temperatures were determined by a tilting test and the corresponding time-temperature experimental points were best fitted to two successive Arrhenius plots intersecting at Θ∼Θmax corresponding to the major protein component for whey proteins and to a minor protein component for egg white albumen. Observations of gel-networks by scanning electron microscopy indicated a wide range of stuctural patterns, depending on the composition of protein solutions. The results are discussed in terms of the temperature of maximal rate of heat-induced conformational changes and of sol-gel state transitions of protein molecules.


DSC egg white proteins SEM sol-gel transition whey proteins 


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  1. 1.
    J. N. De Wit, Developments in Dairy Chemistry, 4, Elsevier Appl. Science, Fox, 1989, p. 285.Google Scholar
  2. 2.
    J. L. Thapon and C. M. Bourgeois, Science et Technique Agroalimentaires, Lavoisier, 1994.Google Scholar
  3. 3.
    P. L. Privalov and D. L. Potekhim, Methods Enzymol., 131 (1986) 4.Google Scholar
  4. 4.
    P. Relkin, Critical Reviews Food Sci. Nutrition, 36 (1996) 565.CrossRefGoogle Scholar
  5. 5.
    T. M. Bikbov Grinberg, V. Y. Grinberg, E. P. Varfolomeyeva and I. B. Likhodzeivskaya, Nahrung, 30 (1996) 369.CrossRefGoogle Scholar
  6. 6.
    T.-X. Liu, Ph. D. Thesis, ENSIA, Université Paris Sud et Université Paris VII, (1993) p. 148.Google Scholar
  7. 7.
    J. C. Gimel, D. Durand and T. Nicolai, Macromolecules 27 (1994) 583.CrossRefGoogle Scholar
  8. 8.
    D. Renard, M. Axelos and J. Lefebvre, Food Macromolecules and Colloids, The Royal Soc. Chem., Cambridge 1995, p. 390.CrossRefGoogle Scholar
  9. 9.
    A. H. Clark, F. J. Judje, J. B. Richards, M. J. Stubbs and A. Sugget, Int. J. Peptide Proteins Res., 17 (1981) 380.CrossRefGoogle Scholar
  10. 10.
    V. R. Harwalkar and M. Kalab, Michwissenschaft, 40 (1985) 31.Google Scholar
  11. 11.
    D. M. Mulvihill and J. E. Kinsella, J. Food Sci., 53 (1988) 231.CrossRefGoogle Scholar
  12. 12.
    M. Langton and A. M. Hermansson, Food Hydrocolloids, 5 (1992) 523.CrossRefGoogle Scholar
  13. 13.
    I. Heertje and F. S. M. van Kleef, Food Microstructure, 5 (1986) 91.Google Scholar
  14. 14.
    E. Doi, Trends Food Sci. Technol., 4 (1993) 1.CrossRefGoogle Scholar
  15. 15.
    P. Relkin, B. Launay and T.-X. Liu, Thermochim. Acta, 3446 (1997) 1.Google Scholar
  16. 16.
    J. Fauquant, J. L. Maubois and A. Pierre, Technol. Laitière, 1028 (1988) 21.Google Scholar
  17. 17.
    P. Relkin, Thermochim. Acta, 246 (1994) 371.CrossRefGoogle Scholar
  18. 18.
    J. N. De Wit and G. A. M. Swinkels, Biochim. Biophys. Acta, 624 (1980) 40.CrossRefGoogle Scholar
  19. 19.
    M. Paulsson and P. Dejmek, J. Dairy Sci., 73 (1990) 45.CrossRefGoogle Scholar
  20. 20.
    J. W. Donovan and C. J. Mapes, J. Sci. Food Agric., 27 (1976) 197.CrossRefGoogle Scholar
  21. 21.
    M. Rossi and A. Schiraldi, Thermochimica Acta, 246 (1992) 115.CrossRefGoogle Scholar
  22. 22.
    T.-X. Liu, P. Relkin and B. Launay, Thermochimica Acta, 246 (1994) 387.CrossRefGoogle Scholar
  23. 23.
    N. Hagolle, P. Relkin, B. Launay and D. G. Dalgleish, Food Hydrocolloids, 11 (1997) 311.CrossRefGoogle Scholar
  24. 24.
    R. K. Richardson and S. B. Ross-Murphy, Int. J. Biol. Macromol., 3 (1981) 315.CrossRefGoogle Scholar
  25. 25.
    P. Relkin, T. Meylheuc, B. Launay and K. Reynal, In Gums and Stabilizers for the Food Industry, Royal Society of Chemistry, 1997 (in press).Google Scholar

Copyright information

© Akadémiai Kiadó 1998

Authors and Affiliations

  1. 1.Laboratoire de Biophysique des Matériaux AlimentairesENSIAFrance
  2. 2.Laboratoire de Génie des Procédés et de l’Hygiène AlimentaireINRAMassyFrance

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