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Aging Clinical and Experimental Research

, Volume 16, Issue 6, pp 425–431 | Cite as

Effects of dietary restriction on age-related changes in the phospholipid fatty acid composition of various rat tissues

  • Ilaria Tamburini
  • Mike F. Quartacci
  • Riccardo Izzo
  • Ettore Bergamini
Original Articles

Abstract

Background and aims: Polyunsaturated fatty acids (PUFAs) are essential components of the cell lipid bilayer and are involved in membrane fluidify and normal functioning, but they are vulnerable to free radical attack. Given the role of oxidative stress in the aging process, age-related changes in phospholipid fatty acid (PLFA) composition in rat liver, kidney and heart were assessed in 3-, 12- and 24-month-old rats fed either ad libitum but only every other day, or daily but only 60% of the quantity normally consumed by age-matched controls. Methods: Lipids were extracted and phospholipids (PLs) were separated using the solid phase extraction technique, then transesterified and assayed by gas-liquid chromatography. Results: Saturated fatty acids (FAs) did not change significantly with age; mono- and bi-unsaturated FAs decreased in the liver and heart, and the ratio of the former to the latter increased in the liver, kidney and heart. PUFAs increased in the liver and heart. As regards individual FAs, 20:1(n-9) decreased in all organs, 14:1 and 18:1(n-7) increased in the kidney and heart, 18:1(n-9) increased in the kidney, 20:2(n-6), 18:2(n-6) and 22:5(n-3) decreased in the liver and heart, 20:3(n-6) decreased in the kidney and increased in the heart. The most abundant PUFAs, 20:4(n-6) and 22:6(n-3), either remained the same or increased with age. The N-9 family increased in the kidney, the N-7 family increased in the kidney and heart, the N-6 family decreased in all three organs, and the N-3 family increased in the liver and kidney. Dietary restriction (DR) significantly counteracted most of these changes, but changes in some FAs [20:2(n-6) in the heart] were magnified by DR and may not be age-related. Conclusions: Most age-related changes (that occurred in the rat liver, kidney and heart and were counteracted by the two different types of DR) may be involved in the mechanism of aging.

Keywords

Aging dietary restriction heart kidney liver membrane function polyunsaturated fatty acid rat 

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References

  1. 1.
    Bergamini E, Bizzarri R, Cavallini G, et al. Ageing and oxidative stress: a role for dolichol in the antioxidant machinery of cell membranes? J Alzheimer Dis 2004; 6: 129–35.Google Scholar
  2. 2.
    Ginsberg BH, Jabour J, Spector AA. Effect of alterations in membrane lipid saturation on the properties of the insulin receptor of Ehrlich ascites cells. Biochim Biophys Acta 1982; 690: 157–64.PubMedCrossRefGoogle Scholar
  3. 3.
    Im WB, Deutchler JT, Spector AA. Effects of membrane fatty acid composition on sodium-dependent phenylalanine transport in Ehrlich cells. Lipids 1979; 14: 1003–8.PubMedCrossRefGoogle Scholar
  4. 4.
    Innis SM, Clandinin MT. Dynamic modulation of mitochondrial membrane physical properties and ATPase activity by diet lipid. Biochem J 1981; 198: 167–75.PubMedGoogle Scholar
  5. 5.
    Spector AA. Yoerk MA. Membrane lipid composition and cellular function. J Lipids Res 1985; 26: 1015–35.Google Scholar
  6. 6.
    Engler MM, Engler MB, Nguyen H. Age-related changes in plasma and tissue fatty acid composition in Fischer 344 rats. Biochem Mol Biol Int 1998; 46: 1117–26.PubMedGoogle Scholar
  7. 7.
    Youdim KA, Deans SG. Beneficial effects of thyme oil on age-related changes in phospholipid C20 and C22 polyunsaturated fatty acid composition of various rat tissues. Biochim Biophys Acta 1999; 1438: 140–6.PubMedCrossRefGoogle Scholar
  8. 8.
    Rao G, Xia E, Richardson A. Effect of age on the expression of antioxidant enzymes in male Fischer 344 rats. Mech Aging Dev 1990; 53: 49–60.PubMedCrossRefGoogle Scholar
  9. 9.
    Cao G, Giovanoni M, Prior RL. Antioxidant capacity in different tissues of young and old rats. Proc Soc Exp Biol Med 1996; 211: 359–65.PubMedCrossRefGoogle Scholar
  10. 10.
    Masoro EJ. Dietary restriction: current status. Aging Clin Exp Res 2001; 13: 261–2.Google Scholar
  11. 11.
    Masoro EJ. Caloric restriction. Aging Clin Exp Res 1998; 10: 173–4.Google Scholar
  12. 12.
    Donati A, Cavallini G, Paradiso C, et al. Age-related changes in the autophagic proteolysis of rat isolated liver cells: effects of antiaging dietary restrictions. J Gerontol 2001; 56: B375–83.CrossRefGoogle Scholar
  13. 13.
    Folch J, Lees M, Sloane-Stanley G. A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 1957; 226: 497–509.PubMedGoogle Scholar
  14. 14.
    Quartacci MF, Cosi E, Navari-Izzo F. Lipids and NADPH-dependent Superoxide production in plasma membrane vesicles from roots of wheat grown under copper deficiency or excess. J Exp Botany 2001; 52: 77–84.CrossRefGoogle Scholar
  15. 15.
    Pamplona R, Portero-Otín M, Ruiz C, Gredilla R, Herrero A, Barja G. Double bond content of phospholipids and lipid peroxidation negatively correlate with maximum longevity in the heart of mammals. Mech Ageing Dev 2000; 112: 169–83.PubMedCrossRefGoogle Scholar
  16. 16.
    Wada H, Gombos Z, Murata N. Enhancement of chilling tolerance of a cyanobacterium by genetic manipulation of fatty acid desaturation. Nature 1990; 347: 200–3.PubMedCrossRefGoogle Scholar
  17. 17.
    Miquel M, Browse J. Arabidopsis requires polyunsaturated lipids for low temperature survival. Proc Natl Acad Sci USA 1993; 90: 6208–12.PubMedCrossRefGoogle Scholar
  18. 18.
    Xiao YF, Ke Q, Wang SY, et al. Single point mutations affect fatty acid block of human myocardial sodium channel alpha subunit Na+ channels. Proc Natl Acad Sci Usa 2001; 98: 3606–11.PubMedCrossRefGoogle Scholar
  19. 19.
    Schmidt A, Wolde M, Thiele C, et al. Endophilin I mediates synaptic vesicle formation by transfer of arachidonate to lysophosphatidic acid. Nature 1999; 401: 133–41.PubMedCrossRefGoogle Scholar
  20. 20.
    Chvojkova’ S, Kazdova’ L, Divisova’ J. Age-related changes in fatty acid composition in muscles. Tohoku J Exp Med 2001; 195: 115–23.CrossRefGoogle Scholar
  21. 21.
    Dolfi C, Bergamini E, Carresi C, et al. The age-related accumulation of dolichol in rat liver may correlate with expectation of life. Biogerontology 2003; 4: 113–8.PubMedCrossRefGoogle Scholar
  22. 22.
    Sohal RJ. Oxidative stress hypothesis of aging. Free Radic Biol Med 2002; 33: 573–4.PubMedCrossRefGoogle Scholar
  23. 23.
    Masoro EJ. Food restriction in rodents: an evaluation of its role in the study of aging. J Gerontol 1988; 43: B59–64.PubMedCrossRefGoogle Scholar
  24. 24.
    Laganiere S, Yu BP. Modulation of membrane phospholipid fatty acid composition by age and food restriction. Gerontology 1993; 39: 7–18.PubMedCrossRefGoogle Scholar
  25. 25.
    Cavallini G, Donati A, Gori Z, Pollera M, Bergamini E. The protection of rat liver autophagic proteolysis from the age-related decline co-varies with the duration of anti-ageing food restriction. Exp Gerontol 2001; 36: 497–506.PubMedCrossRefGoogle Scholar
  26. 26.
    Del Roso A, Vittorini S, Cavallini G, et al. Ageing-related changes in the in vivo function of rat liver macroautophagy and proteolysis. Exp Gerontol 2003; 38: 519–27.PubMedCrossRefGoogle Scholar
  27. 27.
    Bordoni A, Biagi PL, Turchetto E, Hrelia S. Aging influence on delta-6-desaturase activity and fatty acid composition of rat liver microsomes. Biochem Int 1988; 17: 1001–9.PubMedGoogle Scholar
  28. 28.
    Kumar VB, Vyas K, Buddhiraju M, Alshahler M, Flood JF, Morley JE. Changes in membrane fatty acids and delta-9 desaturase in senescence accelerated (SAMP-8) mouse hippocampus with aging. Life Sci 1999; 65: 1657–62.PubMedCrossRefGoogle Scholar
  29. 29.
    Maniongui C, Blond JP, Ulmann L, Durand G, Poisson JP, Bezard J. Age-related changes in delta-6 and delta-5 desaturase activities in rat liver microsomes. Lipids 1993; 28: 291–7.PubMedCrossRefGoogle Scholar
  30. 30.
    Beier K, Volkl A, Fahimi HD. The impact of aging on enzyme proteins of rat liver peroxisomes: quantitative analysis by immunoblotting and immunoelectron microscopy. Virchows Arch B Cell Pathol Incl Mol Pathol 1993; 63: 139–46.PubMedCrossRefGoogle Scholar
  31. 31.
    Legakis JE. Koepke JI. Jedeszko C. et al. Peroxisome senescence in human fibroblasts. Mol Biol Cell 2002; 13: 4243–55.PubMedCrossRefGoogle Scholar
  32. 32.
    Locci Cubeddu T, Masiello P, Pollera M, Bergamini E. Effects of antilipolytic agents on rat liver peroxisomes and peroxisomal oxidative activities. Biochim Biophys Acta 1985; 839: 96–104.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Internal Publishing Switzerland 2004

Authors and Affiliations

  • Ilaria Tamburini
    • 1
  • Mike F. Quartacci
    • 2
  • Riccardo Izzo
    • 2
  • Ettore Bergamini
    • 1
  1. 1.Centro di Ricerca Interdipartimentale “Biologia e Patologia dell’Invecchiamento”PisaItaly
  2. 2.Dipartimento di Chimica e Biotecnologie AgrarieUniversity of PisaPisaItaly

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