Biochemical and Molecular Characterization of Mesorhizobium ciceri Containing acdS Gene
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The enzyme ACC deaminase encoded by acdS gene is known to play a significant role in sustaining plant growth and development under stress conditions by reducing stress induced ethylene production inside the plant. In the present investigation, Mesorhizobium ciceri isolates from chickpea plants growing under drought conditions were screened for the presence of acdS gene. Full length acdS gene from one of the isolates was successfully amplified. This is the first report showing presence of acdS gene in Mesorhizobium ciceri. Gene sequence analysis revealed that there were deletions at two positions in this gene as compared to M. loti acdS gene.
Key wordsabiotic stress acdS ACC deaminase ethylene Mesorhizobium ciceri
1-aminocyclopropane -1-carboxylic acid
yeast extract mannitol agar
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- 8.Uchiumi T, Oowada T, Itakura M, Mitsui H, Nukui N, Dawadi P, Kaneko T, Tabata S, Yokoyama T, Tejima T, Saeki K, Oomori H, Hayashi M, Maekawa T, Sriprang R, Murooka V, Tajima S, Simomura K, Nomura M, Suzuki A, Shimoda S, Sioya K, Abe M & Minamisawa K, J Bacteriol, 186 (2004) 2439.PubMedCrossRefGoogle Scholar
- 9.Vincent JM, A manual for the practical study of the root nodule bacteria, Blackwell Scientific Publication, Oxford and Edinburg (1970).Google Scholar
- 10.Smibert R & Krieg NR, In Methods for general and molecular bacteriology, (P Gerhardt, RGE Murray, WA Wood, NR Krieg, Editors), American Society for Microbiology, Washington (1994) p 611.Google Scholar