Advertisement

Global Analysis of Rice Tungro Spherical Virus Coat Proteins Reveals New Roles in Evolutionary Consequences

  • Satendra K. Mangrauthia
  • P. Malathi
  • S. M. Balachandran
  • C. S. Reddy
  • B. C. Viraktamath
Short Communication

Abstract

Rice tungro disease is caused by a combination of two viruses: Rice tungro spherical virus (RTSV) and Rice tungro bacilliform virus (RTBV). RTSV has a capsid comprising three coat proteins (CP) species. Three CP genes of RTSV-AP isolate were sequenced and compared with 9 other isolates reported worldwide for their phylogenetic survey of recombination events which revealed that in general Indian isolates are forming one separate cluster while those of Philippines and Malaysia forming a different cluster. A significant proportion of recombination sites were found in the CP1 gene, followed by CP2 and CP3 suggesting that it is a major phenomenon in the evolution of various isolates of RTSV. Some interesting domains and motifs such as; 3,4-dihydroxy-2-butanone 4-phosphate synthase in CP1, Type 1 glutamine amidotransferase domain and RNA binding motifs in CP2, domains of receptor proteins in CP3, and glycosylation motif in CP2 and CP3 were also obtained in RTSV coat protein. In addition, simple modular architecture research tool (SMART) analysis of coat proteins of RTSV predicted the coat protein domain of calicivirus suggesting evolutionary linkages between plant and animal viruses. This study provides an opportunity to establish the molecular evolution and sequence-function relationship of RTSV.

Key words

RTSV domain prediction CF motifs phylogeny 

Abbreviations

CDD

conserved domain database

CP

coat protein

SMART

simple modular architecture research tool

RTBV

Rice tungro bacilliform virus

RTSV

Rice tungro spherical virus

RTD

Rice tungro disease

RDP

Recombination detection programme

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Muralidharan K, Krishnaveni D, Rajarajeshwari N V L & Prasad A S R, Curr Sci, 65 (2003) 1143.Google Scholar
  2. 2.
    Hull R, Annu Rev Phytopathol, 34 (1996) 275.PubMedCrossRefGoogle Scholar
  3. 3.
    Letunic I, Doerks T & Bork P, NucleicAcids Res, 37 (2009) D229.CrossRefGoogle Scholar
  4. 4.
    Hulo N, Bairoch A, Bulliard V, Cerutti L, Cuche B A, de Castro E, Lachaize C, Langendijk-Genevaux P S & Sigrist C J, Nucleic Acids Res, 36 (2008) D245.PubMedCrossRefGoogle Scholar
  5. 5.
    Marchler-Bauer A, Anderson J B, Chitsaz F, Derbyshire M K, DeWeese-Scott C, Fong J H, Geer L Y, Geer R C, Gonzales N R, Gwadz M, He S, Hurwitz D I, Jackson J D, Ke Z, Lanczycki C J, Liebert C A, Liu C, Lu F, Lu S, Marchler G H, Mullokandov M, Song J S, Tasneem A, Thanki N, Yamashita R A, Zhang D, Zhang N & Bryant S H, Nucleic Acids Res, 37 (2009) (D) 205.CrossRefGoogle Scholar
  6. 6.
    Roossinck M J, Annu Rev Phytopathol, 35 (1997) 191.PubMedCrossRefGoogle Scholar
  7. 7.
    Hall T A, Nucleic Acids Symp Ser, 41 (1999) 95.Google Scholar
  8. 8.
    Thompson J D, Gibson T J, Plewniak F, Jeanmougin F & Higgins D G, Nucleic Acids Res, 24 (1997) 4876.CrossRefGoogle Scholar
  9. 9.
    Martin D P, Posada D, Crandall K A & Williamson C, AIDS Res Hum Retroviruses, 21 (2005) 98.PubMedCrossRefGoogle Scholar
  10. 10.
    Mangrauthia S K, Parameswari B, Jain R K & Praveen S, Biochem Genet, 46 (2008) 835.PubMedCrossRefGoogle Scholar
  11. 11.
    Grohmann G, Glass R I, Gold J, James M, Edwards P, Borg T, Stine S E, Goldsmith C & Monroe S S, J Clin Microbiol, 29 (1991) 544.PubMedGoogle Scholar
  12. 12.
    Taheri P & Tarighi S, J Plant Physiol, 167 (2010) 201.PubMedCrossRefGoogle Scholar
  13. 13.
    Mangrauthia S K, Singh P & Praveen S, Mot Biotechnol, 44 (2010) 22.CrossRefGoogle Scholar
  14. 14.
    Druka A, Burns T, Zhang S & Hull R, J Gen Virol, 77 (1996) 1975.PubMedCrossRefGoogle Scholar
  15. 15.
    Vigerust D J & Shepherd V L, Trends Microbiol, 15 (2007) 211.PubMedCrossRefGoogle Scholar

Copyright information

© Springer 2010

Authors and Affiliations

  • Satendra K. Mangrauthia
    • 1
  • P. Malathi
    • 1
  • S. M. Balachandran
    • 1
  • C. S. Reddy
    • 1
  • B. C. Viraktamath
    • 1
  1. 1.Directorate of Rice ResearchHyderabadIndia

Personalised recommendations