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Journal of Plant Biochemistry and Biotechnology

, Volume 17, Issue 1, pp 107–109 | Cite as

ISSR and RAPD Markers Assessed Genetic Variation of Aerides maculosum — an Epiphytic Orchid from Goa, India

  • G. V. Parab
  • S. Krishnan
  • M. K. Janarthanam
  • K. R. Sivaprakash
  • Ajay Parida
Short Communication

Abstract

Aerides maculosum Lindl is one of the most important orchids valued for its beautiful inflorescence/flowers. The present study aimed to understand the level of genetic variation among the populations of A. maculosum using RAPD and ISSR markers. Among the 35 primers tested, 13 RAPD and 6 ISSR primers were selected for the analysis. Total of 101 RAPD and 40 ISSR fragments were generated. High level of polymorphism was recorded in RAPD (90.45%) compared to ISSR (72.85 %). Nei’s average genetic identity values for different populations of A. maculosum- ranged from 0.465 to 0.762 (RAPD), while for ISSR it ranged from 0.475 to 0.975. The present study provides important insights about genetic variation in A. masculosum and may facilitates the conservation and management of this species.

Key words

Aerides maculosum RAPID ISSR genetic variation 

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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • G. V. Parab
    • 1
  • S. Krishnan
    • 1
  • M. K. Janarthanam
    • 1
  • K. R. Sivaprakash
    • 2
  • Ajay Parida
    • 2
  1. 1.Department of BotanyGoa UniversityGoaIndia
  2. 2.M. S. Swaminathan Research FoundationTaramaniIndia

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