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Cloning and Characterization of Partial Promoter of HMGCoA Reductase from Andrographis paniculata (Burm.f.) Wall.ex Nees — A Tropical Medicinal Plant

  • K. Seetha
  • N. S. Banerjee
  • R. V. Omkumar
  • M. G. Purushothama
Short Communication

Abstract

Hydroxymethylglutaryl-CoA (HMGCoA) reductase, a key enzyme of the mevalonate pathway is known to play a key role in the biosynthesis of terpenoids. Here we describe, the cloning and characterization of hmgr1 promoter from Andrographis paniculata. A 710 bp partial promoter fragment including a putative TATA box, 5’ UTR and 5’ coding sequence of the gene was cloned by amplifying the upstream sequences using PCR with appropriate primers. Analysis of the promoter sequence against PLACE (Plant cis-acting Regulatory DNA Elements) database showed the presence of certain putative light dependent regulatory cis-elements in the sequence along with TATA and CAAT boxes. RT-PCR detected GUS transcripts in transgenic plants.

Key words

Andrographis paniculata HMGCoA reductase promoter genome walking 

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Copyright information

© Springer 2005

Authors and Affiliations

  • K. Seetha
    • 1
  • N. S. Banerjee
    • 2
  • R. V. Omkumar
    • 3
  • M. G. Purushothama
    • 1
  1. 1.Plant Molecular Biology LaboratoryRajiv Gandhi Centre for BiotechnologyKeralaIndia
  2. 2.Biochemistry and Molecular GeneticsUniversity of Alabama at BirminghamBirmingham, AlabamaUSA
  3. 3.Neurobiology LaboratoryRajiv Gandhi Centre for BiotechnologyKeralaIndia

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