Differentiation of Isolates of Cotton Root Rot Pathogens Rhizoctonia solani and R. bataticola Using Pathogenicity and RAPD Markers

  • D. Monga
  • S. S. Rathore
  • C. D. Mayee
  • T. R. Sharma
Short Communication


Twelve isolates of Rhizoctonia solani and fifteen isolates of R. bataticola causing root rot of cotton were studied for their pathogenicity and genetic diversity using RAPD markers. Pathogenicity of these isolates varied when tested on Gossypium arboreum variety RG-8. The similarity value of RAPD profiles in R. solani isolates ranged from 0.35 to 1.00 with an average of 0.67 among all the isolates and in R. bataticola it ranged from 0.27 to 1.00 with an average of 0.63 among all the isolates. Eighteen primers were used to fingerprint the individual isolates. The cluster analysis using unweighted primer group method with arithmetic average could distinguish R. solani and R. bataticola isolates into two separate fingerprint groups.

Key words

cotton Rhizoctonia solani R. bataticola RAPD markers 


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  1. 1.
    Sobati JG & Sharma RL, Indian Phytopathol, 42 (1992) 506.Google Scholar
  2. 2.
    Monga D & Sheoraj, Indian Phytopathol, 47 (1994) 403.Google Scholar
  3. 3.
    Sharma TR, Indian J Biotech, 2 (2003) 99.Google Scholar
  4. 4.
    Reader U & Broda P, Lett Appl Microbiol, 1 (1985) 17.CrossRefGoogle Scholar
  5. 5.
    Yal IV & Nelson RJ, Winboot: A program for performing bootstrap analysis of binary data to determine the confidence of UPGMA-based dendrograms, IRRI, Manilla, Philippines (1996).Google Scholar
  6. 6.
    Kuninaga S, In Rhizoctonia species: Taxonomy, molecular biology, ecology, pathology and disease control. (B Sneh, S Jabaji-Hare, S Neate, G Dijst Editors), Kluwer Academic Publishers, Dordrecht, The Netherlands, (1996) pp 73–80.Google Scholar
  7. 7.
    Vilgalys R & Gonzalez D, Phytopathology, 80 (1990) 151.CrossRefGoogle Scholar
  8. 8.
    Toda T, Hyakumachi M, Suga H, Kageyama K, Tanaka A & Tani T, Eur J Plant Pathol, 105 (1999) 835.CrossRefGoogle Scholar
  9. 9.
    Neeraja CN, Vijayabhanu N, Shenoy VV, Reddy CS & Sharma NP, J Plant Biochem Biotechnol, 11 (2000) 43.CrossRefGoogle Scholar
  10. 10.
    Sussel AAB, Fenille RC, Kuramae EE, Souza NL & De souza NL, Phytopathologica, 27(2001) 348.Google Scholar
  11. 11.
    Zhu X, Zhou E, Yi RH, Liang CY, Zhu XR & Zhoue EX, J Trop Subtrop Botany, 10 (2002) 161.Google Scholar

Copyright information

© Springer 2004

Authors and Affiliations

  • D. Monga
    • 1
  • S. S. Rathore
    • 1
  • C. D. Mayee
    • 2
  • T. R. Sharma
    • 3
  1. 1.Central Institute for Cotton Research ( CICR )SirsaIndia
  2. 2.Krishi BhawanNew DelhiIndia
  3. 3.National Research Centre on Plant Biotechnology, IARINew DelhiIndia

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