Inhibition of H+ Extrusion by Phosphocreatine in Candida albicans

  • Nikhat Manzoor
  • Md Mahfuzul Haque
  • Luqman A. Khan
Short Communication


Vanadate, a potent inhibitor of P-type ATPases, reduces the electrochemical gradient considerably. H+-extrusion in cells of Candida albicans, a pathogenic yeast, was strongly inhibited in the presence of 25mM phosphocreatine (PCr) by about 83%. H+-extrusion was further inhibited by 25 mM PCr in the presence of vanadate; 89% with 1 mM, 92% with 2 mM and 99% with 5 mM vanadate. 2 mM vanadate caused 90%, 92% and 96% inhibition in the presence of 20 mM, 30 mM and 40 mM PCr, respectively. Creatine (Cr) had a negligible effect on H+ - extrusion. The inhibition caused by 1 mM, 2 mM and 5 mM vanadate alone was 66%, 77% and 88%, respectively. PCr and vanadate inhibit proton extrusion with almost equal magnitude. It can be concluded that phosphate moiety of PCr interacts with the ATPase and is similar to vanadate interaction. Since PCr is having such a drastic inhibitory effect on ATPase activity we can say that it is playing a significant role in holding a check on this pathogenic fungus in healthy human hosts.

Key words

Candida albicans H+-extrusion vanadate phosphocreatine creatine 







creatine kinase


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  1. 1.
    Odds FC, CRC Crit Rev Microbiol, 12 (1985) 45.CrossRefGoogle Scholar
  2. 2.
    Monk BC & Perlin OS, CRC Crit Rev Microbiol, 20 (1994) 209.CrossRefGoogle Scholar
  3. 3.
    Manzoor N, Amin M & Khan LA, Indian J Exp Bioi, 40 (2002) 785.Google Scholar
  4. 4.
    Borst-Pauwels GWFH & Peters PHJ, Biochim Biophys Acta, 642 (1981) 173.PubMedCrossRefGoogle Scholar
  5. 5.
    Bowman BJ & Siayman CW, J Bioi Chem, 254 (1979) 2928.Google Scholar
  6. 6.
    Kaur S, Mishra P & Prasad R, Biochim Biophys Acta, 972 (1988) 277.PubMedCrossRefGoogle Scholar

Copyright information

© Springer 2004

Authors and Affiliations

  • Nikhat Manzoor
    • 1
  • Md Mahfuzul Haque
    • 1
  • Luqman A. Khan
    • 1
  1. 1.Department of BiosciencesJamia Millia IslamiaNew DelhiIndia

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