Skip to main content
SpringerLink
Log in

RAPD Analysis of Genetic Diversity in Indian Land Races of Rice (Oryza sativa L)

  • Published:
Journal of Plant Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

The study aims at determining genetic diversity in a set of land races in comparison to a representative sample of improved rice Varieties, using random amplified polymorphic DNA (RAPD). Analysis of 36 accessions using 10 arbitrary decamer random primers, revealed 97.1 6% polymorphism. Similarity values among the land races ranged from 0.58 to 0.89 indicating wide diversity.The extent of diversity of the accessions was assessed using heterozygosity arithmetic mean value in land races (0.71) and cultivars (0.54). Marker Index (MI) revealed higher values in land races (120.83) than improved varieties (51.74).The land races and improved varieties formed separate clusters at 0.65 similarity suggesting that genetically distant land races could be potentially valuable sources for enlarging and enriching the genepool of improved varieties.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Tanksley SD & McCouch SR, Science, 277 (1997) 1063.

    Article  PubMed  CAS  Google Scholar 

  2. Stobdan T, Khanna VK, Singh NK & Mani SC, Rice Biotech Quart, 40 (1999) 18.

    Google Scholar 

  3. Raghunathachari P, Khanna VK, Singh US & Singh NK, Curr Sci, 79 (2000) 994.

    CAS  Google Scholar 

  4. Wang ZY & Tanksley SD, Genome, 32 (1989) 1113.

    Article  CAS  Google Scholar 

  5. Wu KS & Tanksley SD, Mol Gen Genet, 241 (1993) 225.

    Article  PubMed  CAS  Google Scholar 

  6. Parsons BJ, Newbury HJ, Jackson MT & Ford-Lloyd BV, Mol Breed, 3 (1997) 115.

    Article  CAS  Google Scholar 

  7. Singh KN, Nandi R, Shanmugasundaram P, Sadasivam S, Huang N, Brar DS & Khush GS, Genet Res and Crop Evol, 46 (1999) 427.

    Article  Google Scholar 

  8. Williams JGK, Kubelik AR, Livak KJ, Rafalski JA & Tingey SV, Nucleic Acids Res, 18 (1990) 6531.

    Article  PubMed  CAS  Google Scholar 

  9. Welsh J & McClelland M, Nucleic Acids Res, 18 (1990) 7213.

    Article  PubMed  CAS  Google Scholar 

  10. Mackill DJ, Crop Sci, 35 (1995) 889.

    Article  CAS  Google Scholar 

  11. Virk PS, Newbury HJ, Jackson MT & Ford-Lloyd BV, Theor Appl Genet, 90 (1995) 1049.

    Article  CAS  Google Scholar 

  12. Cho YC, Shin YS, Ahn SN, Gregorio GB, Kang KH, Brar DS & Moon HP, Korean J Crop Sci, 44 (1999) 26.

    Google Scholar 

  13. Ghareyazie B, Huang N, Second G & Bennett J, Theor Appl Genet, 91 (1995) 218.

    Article  CAS  Google Scholar 

  14. Cao D & Oard JH, Crop Sci, 37 (1997) 1630.

    Article  CAS  Google Scholar 

  15. Davierwala AP, Chowdhari KV, Shiv Kumar, Reddy APK, Ranjekar PK & Gupta VS, Genetica, 108 (2000) 269.

    Article  PubMed  CAS  Google Scholar 

  16. Jena KK & Kochert G, Plant Mol Biol, 16 (1991) 831.

    Article  PubMed  CAS  Google Scholar 

  17. Rohlf FJ, NTSYS. Numerical taxonomyandmultivariatea nalysis system. Version 2.0, (1993).

    Google Scholar 

  18. Nei M, Proc Natl Acad Sci, USA, 70 (1973) 3321.

    Article  PubMed  CAS  Google Scholar 

  19. Powell W, Morgante M, Andre C, Hanafey M, Vogel J, Tingey S. & Rafalski A, Mol Breed, 2 (1996) 225.

    Article  CAS  Google Scholar 

  20. Yang GP, Saghai Maroof MA, Xu CG, Zhang O & Biyashev RM, Mol Gen Genet, 245 (1994) 187.

    Article  PubMed  CAS  Google Scholar 

  21. Fuentes JL, Escovar F, Alvarez A, Gallego G, Duque MC, Ferrer M, Deus JE & Tohme JM, Euphytica, 109 (1999) 107.

    Article  CAS  Google Scholar 

  22. Virk PS, Zhu J, Newbury HJ, Bryan GJ, Jackson MT & Ford-Lloyd BV, Euphyfica, 112 (2000) 275.

    Article  CAS  Google Scholar 

  23. Fanniza G, Colonna G, Resta P & Ferrara G, Euphytica, 107 (1999) 45.

    Article  Google Scholar 

  24. Oka HI, In Origin of cultivated rice, Japan Scientific Societies Press, Tokyo, Elsevier Science Publishers, Amsterdam (1988) p 114.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. National Professor Project, Directorate of Rice Research, Rajendranagar, Hyderabad, 500 030, India

    C. N. Neeraja, N. Sarla & E. A. Siddiq

Authors
  1. C. N. Neeraja
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. N. Sarla
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. E. A. Siddiq
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Neeraja, C.N., Sarla, N. & Siddiq, E.A. RAPD Analysis of Genetic Diversity in Indian Land Races of Rice (Oryza sativa L). J. Plant Biochem. Biotechnol. 11, 93–97 (2002). https://doi.org/10.1007/BF03263142

Download citation

  • Received:

  • Revised:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF03263142

Keywords

Search

Navigation